Poly[oligo(ethylene glycol) methacrylate]-b-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNA

Abstract

We report on the preparation of novel and multifunctional hybrid spherical-shaped nanostructures involving a double-hydrophilic block copolymer, namely the neutral cationic poly[oligo(ethylene glycol) methacrylate]-b-poly[(vinyl benzyl trimethylammonium chloride)] (POEGMA-b-PVBTMAC) diblock copolymer, initially complexed with hydrophilic anionic magnetic nanoparticles (MNPs), and subsequently, with short deoxyribonucleic acid (113 bases DNA). The POEGMA-b-PVBTMAC copolymer, the copolymer/MNPs and the copolymer/MNPs/DNA tricomponent hybrid electrostatic complexes were studied by dynamic/electrophoretic light scattering (DLS/ELS) and cryogenic transmission electron microscopy (cryo-TEM) techniques for the determination of their structure and solution properties. The MNPs were complexed efficiently with the oppositely charged diblock chains, leading to well-defined hybrid organic–inorganic spherical-shaped nanostructures. A significant aggregation tendency of the MNPs is noticed in cryo-TEM measurements after the electrostatic complexation of DNA, implying an accumulation of the DNA macromolecules on the surface of the hybrid tricomponent complexes. Magnetophoretic experiments verified that the MNPs maintain their magnetic properties after the complexation initially with the copolymer, and subsequently, within the block polyelectrolyte/MNPs/DNA nanostructures.

Non-viral Vectors in Gene Therapy: Recent Development, Challenges, and Prospects

“…Chroni et al . synthesized a magnetopolyplex incorporating a diblock copolymer, hydrophilic anionic magnetic nanoparticles, and short deoxyribonucleic acid ( 78 ). The intensity of this magnetopolyplex measured through dynamic light scattering was constant at low NaCl concentrations followed by a gradual decrease as ionic strength increased because a higher ionic strength produced a weaker interaction between DNA and carrier, reducing the amount of complexes.…”

Amino-modified 2-oxazoline copolymers for complexation with DNA

“…2,[6][7][8][9][10][11][12] There are also other polymeric systems known for the preparation of polyplexes. [13][14][15][16][17] In the design of polyplexes, great emphasis is put on the ability of macromolecules to electrostatically bind and condense nucleic acids into nanometer sized particles. Many studies have shown that among others the polymer molar mass, topology, type of cationic functionality and charge density as well as polyplex surface charge and its conformation in the solution influenced the cytotoxicity and efficiency of transfection of such carriers.…”

Amphiphilic P(OEGMA-co-DIPAEMA) Hyperbranched Copolymer/Magnetic Nanoparticle Hybrid Nanostructures by Co-Assembly

“…Therefore, they are being extensively developed and constructed due to their multi-functionality for the cure of various life-threatening diseases. A wide range of research has been reported to procure the effectiveness of such materials in theranostic applications [1][2][3][4][5][6][7][8][9]. Polymer nanomedicine is based on the development of copolymers that are biodegradable and biocompatible, and provides bioavailability and colloidal stability to the formed multifunctional hybrid nanomaterials and entrapped species.…”