Poly-o-aminophenol as a laccase mediator and influence of the enzyme on the polymer electrodeposition

Pałys, Barbara; Marzec, M.; Rogalski, Jerzy

doi:10.1016/j.bioelechem.2010.06.008

Cited by 18 publications

(31 citation statements)

References 52 publications

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“…Regarding PAP films, the evidence of peaks at about 1400 and 1600 cm −1 Raman shift were assigned to quinoid groups of 3-aminophenoxazone (APZ) and to the aromatic ring/-C N-in quinonimine units, respectively [62][63][64], from the PAP films. The PAP electropolymerization is expected to yield two main products: APZ and 2,2 1 -dihydroxyazobenzene (DHAB).…”

Section: Raman Spectrometrymentioning

confidence: 99%

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Protein-responsive polymers for point-of-care detection of cardiac biomarker

Moreira

Sharma

Dutra

et al. 2014

Sensors and Actuators B: Chemical

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This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made respon-sive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing prot ein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications.Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the mate-rial to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The d evices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 µg/mL, respectively, with detection limits of 1.5 and 0.8 µg/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivit y and selectivity.

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Section: Raman Spectrometrymentioning

confidence: 99%

“…APZ is cyclic dimer, by the simultaneous N-C and O-C coupling of AP monomer units, obtained especially under acidic pH conditions. APZ can be oxidized further at the electrode forming the ladder structured polymer [62]. DHAB is formed mostly in neutral and basic solutions.…”

Section: Raman Spectrometrymentioning

confidence: 99%

Protein-responsive polymers for point-of-care detection of cardiac biomarker

Moreira

Sharma

Dutra

et al. 2014

Sensors and Actuators B: Chemical

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“…At pH values between 3 and 7, a diminishing in the current of the peak system d-d , assigned to the film formation, was observed. Barbero et al [10] remark that if extreme care is not taken in the preparation of a POAP film, not only in the concentration but also in the potential ranges, the possibility of side reactions and Potentiodynamic cycling (−0.1 V and 0.9 V versus Ag/AgCl) [23] consequently "side" polymers increases, and the real structures of the films obtained could be quite complex. Then, the best conditions proposed in [10] for obtaining a reproducible POAP film are the repetitive cycling between −0.25 V and 0.70 V (SCE) of an o-AP aqueous acid solution (pH 1) and an o-AP concentration less than 20 mM.…”

Section: Electropolymerization Of O-aminophenol In Acidic Mediamentioning

confidence: 99%

“…Thin nonconducting POAP films synthesized in neutral media are also employed as protective layers for biosensor applications. In general, POAP films are simultaneously synthesized with different electroactive materials such as, hemoglobin, quinhydrone, carbon nanotubes, prussian blue, and other polymers such as poly(o-phenylenediamine) and polypyrrole, which act as electron mediators [18,23,[28][29][30][31][32][33][34][35][36][37], to build different biosensors. POAP is also widely used as Figure 11: IR spectra of (a) electrochemically prepared POAP, (b) 3-aminophenoxazone [10].…”

Section: Electropolymerization Of O-aminophenol In Alkaline Andmentioning

confidence: 99%

“…Furthermore, from the fact that the absorption peak, which is assigned to the stretching vibration of the C=O bonds of aromatic keto groups, was observed at 1670 cm −1 , not only the partially hydrolyzed structure shown in Figure 7 was proposed for POAP in [16] but also the structure shown in Figure 8, which was assumed to proceed from a polymerization via C-N=C bonds, was proposed as possible structure of POAP. The role of the laccase on the electrochemical synthesis of POAP from 0.1 M phosphate buffer (pH 5.5) solutions is described in [23]. Also, it is demonstrated in [23] that laccase can be utilized as a polymerization initiator with no need of electrochemical monomer oxidation.…”

Section: Spectroscopic Characterization Of Poap Films Synthesized In mentioning

confidence: 99%

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Electrosynthesis and Spectroscopic Characterization of Poly(o-Aminophenol) Film Electrodes

Tucceri

Arnal

Scian

2012

ISRN Polymer Science

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This review, which is divided into three parts, concerns electrochemical synthesis, spectroscopic characterization, and formation mechanisms of poly(o-aminophenol) (POAP) film electrodes. The first part of this review is devoted to describe the electropolymerization process of o-aminophenol on different electrode materials and in different electrolyte media by employing both potentiodynamic and potentiostatic methods. The second part refers to spectroscopic studies carried out by different authors to both, identify the products of the o-aminophenol electrooxidation and elucidate the chemical structure of poly(o-aminophenol) film electrodes. The third part shows the different mechanisms formulated to interpret the POAP films formation from both acid and basic solutions of o-AP. Also, some electrochemical and spectroscopic data which allowed to propos the corresponding formation mechanisms, especially in basic media, are described.

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