“…This partially compensates for exhausting pH sensitivity of the response mentioned for the analogs studied. Similar trends were discussed in the application of phenothiazine dyes for mediation of the oxidation of small molecules (see reviews [ 31 , 36 , 58 ]).…”
Section: Resultssupporting
confidence: 60%
“…These changes were observed until the 25 th cycle, after which the currents started decreasing due to blocking the GCE surface with the polymerization products, which complicated access of the monomers to the electrode interface. Such non-regular changes in the peak currents are typical for the electropolymerization of the phenothiazine and phenoxazine derivatives [ 58 , 59 ]. The dependence of the oxidation/reduction peak currents on the number of electropolymerization cycles is presented in Figure 2 b.…”
Section: Resultsmentioning
confidence: 99%
“…Diaminated derivatives demonstrated more complicated voltammograms with the peaks of monomeric and polymeric forms comparable in height. Structurally relative thionine and azure dyes exerted the activity in electropolymerization sensitive to the steric loading of the amino groups [ 29 , 32 , 33 , 37 , 58 ]. Cyclic voltammograms of Azure A and Azure B demonstrated similar broad peaks of the polymer accumulation but the quantities of appropriate products assessed from the currents were significantly lower.…”
A novel voltammetric sensor based on a self-assembled composite formed by native DNA and electropolymerized N-phenyl-3-(phenylimino)-3H-phenothiazin-7-amine has been developed and applied for sensitive determination of doxorubicin, an anthracycline drug applied for cancer therapy. For this purpose, a monomeric phenothiazine derivative has been deposited on the glassy carbon electrode from the 0.4 M H2SO4-acetone mixture (1:1 v/v) by multiple potential cycling. The DNA aliquot was either on the electrode modified with electropolymerized film or added to the reaction medium prior to electropolymerization. The DNA entrapment and its influence on the redox behavior of the underlying layer were studied by scanning electron microscopy and electrochemical impedance spectroscopy. The DNA–doxorubicin interactions affected the charge distribution in the surface layer and, hence, altered the redox equilibrium of the polyphenothiazine coating. The voltametric signal was successfully applied for the determination of doxorubicin in the concentration range from 10 pM to 0.2 mM (limit of detection 5 pM). The DNA sensor was tested on spiked artificial plasma samples and two commercial medications (recovery of 90–95%). After further testing on real clinical samples, the electrochemical DNA sensor developed can find application in monitoring drug release and screening new antitumor drugs able to intercalate DNA.
“…This partially compensates for exhausting pH sensitivity of the response mentioned for the analogs studied. Similar trends were discussed in the application of phenothiazine dyes for mediation of the oxidation of small molecules (see reviews [ 31 , 36 , 58 ]).…”
Section: Resultssupporting
confidence: 60%
“…These changes were observed until the 25 th cycle, after which the currents started decreasing due to blocking the GCE surface with the polymerization products, which complicated access of the monomers to the electrode interface. Such non-regular changes in the peak currents are typical for the electropolymerization of the phenothiazine and phenoxazine derivatives [ 58 , 59 ]. The dependence of the oxidation/reduction peak currents on the number of electropolymerization cycles is presented in Figure 2 b.…”
Section: Resultsmentioning
confidence: 99%
“…Diaminated derivatives demonstrated more complicated voltammograms with the peaks of monomeric and polymeric forms comparable in height. Structurally relative thionine and azure dyes exerted the activity in electropolymerization sensitive to the steric loading of the amino groups [ 29 , 32 , 33 , 37 , 58 ]. Cyclic voltammograms of Azure A and Azure B demonstrated similar broad peaks of the polymer accumulation but the quantities of appropriate products assessed from the currents were significantly lower.…”
A novel voltammetric sensor based on a self-assembled composite formed by native DNA and electropolymerized N-phenyl-3-(phenylimino)-3H-phenothiazin-7-amine has been developed and applied for sensitive determination of doxorubicin, an anthracycline drug applied for cancer therapy. For this purpose, a monomeric phenothiazine derivative has been deposited on the glassy carbon electrode from the 0.4 M H2SO4-acetone mixture (1:1 v/v) by multiple potential cycling. The DNA aliquot was either on the electrode modified with electropolymerized film or added to the reaction medium prior to electropolymerization. The DNA entrapment and its influence on the redox behavior of the underlying layer were studied by scanning electron microscopy and electrochemical impedance spectroscopy. The DNA–doxorubicin interactions affected the charge distribution in the surface layer and, hence, altered the redox equilibrium of the polyphenothiazine coating. The voltametric signal was successfully applied for the determination of doxorubicin in the concentration range from 10 pM to 0.2 mM (limit of detection 5 pM). The DNA sensor was tested on spiked artificial plasma samples and two commercial medications (recovery of 90–95%). After further testing on real clinical samples, the electrochemical DNA sensor developed can find application in monitoring drug release and screening new antitumor drugs able to intercalate DNA.
“…“According to the World Health Organization diabetes is a leading cause of blindness, kidney failure, heart attacks, strokes, and lower limb amputation” [ 4 ]. These associated health risks have led to an emerging interest in the advancement of straightforward early diagnosis and treatment techniques for diabetes mellitus [ 5 ]. To this end, researchers have gained considerable interest in electrochemical glucose sensors, because of their cost effectiveness, high sensitivity, high stability, and ease of handling during experiments [ [6] , [7] , [8] , [9] ], Due to these advantages, highly-sensitive glucose sensors are used in diverse fields, such as medical diagnostics [ 10 ], agro-food technology [ 11 ], and pharmaceuticals [ 12 ].…”
“…Nowadays, electrochemical polymerization has attracted high attention in electrode modification due to a number of obvious advantages such as: wide variation of the electrode size and shape; compatibility with the format of microdevice; easy control of the deposited mass and coating thickness by variation of the number of potential cycles; satisfactory repeatability and simplicity of the electrode surface regeneration [13]. Taking into account the mechanism of CuAAC [14], one of the most attractive and promising monomers for the creation of "clickable" electroconductive coatings on the electrode surface is 3-ethynylthiophene (3-Eth).…”
An original electrodeposited polymer film, based on 3-ethynylthiophene, for covalent immobilization of proteins via "click" reaction of copper catalyzed azidealkyne cycloaddition was developed. The best characteristics have demonstrated the layer-by-layer immobilized 3,4-ethylendioxythiophene-3-ethynylthiophene composite film. The bovine serum albumin and antibodies have been successfully immobilized on the surface of planar plati-num electrode. The approach has been applied to labelfree electrochemical immunosensors for Escherichia coli and Staphylococcus aureus determination. The immunosensors demonstrated LODs 7.2 CFU/mL for Escherichia coli and 15.9 CFU/mL for Staphylococcus aureus, linear range 10 2 -10 6 CFU/mL and could be promising for environmental and food control.
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