Poly(L-lactic acid) nanofibers containing Cissus quadrangularis induced osteogenic differentiation in vitro

Parvathi, K; Krishnan, Amit G; Anitha, A; Jayakumar, R.; Nair, Manitha B.

doi:10.1016/j.ijbiomac.2017.11.094

Cited by 30 publications

(27 citation statements)

References 35 publications

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“…RSM gave suitable conditions to produce nanofibers with the best morphology and diameter. Based on RSM results, the optimum solution concentrations were obtained, and black seed extract with different concentrations (10,15,20, and 25% based on the solid content of the solution) was added to PLA and PCL solutions. Herbal extract (NS) with PLA (8%) were transferred into 5 ml syringe, and electrospun under optimum conditions (voltage 20 kV, feed rate 0.8 ml/hr and distance 15 cm) and nanofibers were formed on the aluminum collector.…”

Section: Polymer Solutions Preparation and Electrospinningmentioning

confidence: 99%

“…Poly(lactic acid) is an aliphatic polyester derived from renewable sources such as cornstarch, sugarcane, and other annually renewable biomass products and wastes, which can be obtained from direct condensation polymerization of lactic acid monomers or the ringopening polymerization of the cyclic lactide dimers. [19,20] It is highly versatile, biocompatible, biodegradable at a slow rate, and semi-crystalline solid. This polymer is widely used in agriculture, packaging, sutures, scaffolds for tissue engineering, and carriers for drug delivery.…”

Section: Introductionmentioning

confidence: 99%

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Electrospun PCL and PLA hybrid nanofibrous scaffolds containing Nigella sativa herbal extract for effective wound healing

Sharifi

Bahrami

Nejad

et al. 2020

J of Applied Polymer Sci

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Black seed (Nigella sativa [NS]) is used in traditional medicine as an antibacterial agent. In this study, novel hybrid scaffolds were manufactured from poly(ɛ‐caprolactone[PCL])/ Poly(lactic acid [PLA]) with NS extract by double‐nozzle electrospinning for wound healing application. Optimal conditions were found using response surface methodology including feed rate 0.8 ml/hr, voltage 20 kV and PLA 8% /PCL 10% concentrations. The effect of NS extract on the properties of the scaffold was evaluated by scanning electron microscopy, contact angle, and mechanical test. The PLA‐PCL/NS 20% beadles, and smooth nanofibrous web was obtained as the optimum scaffold with an average diameter of 638 ± 69 nm and the contact angle of 44°. In addition, the biological properties of the scaffolds such as antibacterial against Staphylococcus aureus (gram‐positive) and Escherichia coli (gram‐negative) bacteria, MTT assay, extract release, and cell growth (human mesenchymal stem cells) were examined. Incorporation of NS extract into the nanofibers caused to enhance the biological properties, cell viability and cell proliferation without toxicity.

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Section: Polymer Solutions Preparation and Electrospinningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrospun PCL and PLA hybrid nanofibrous scaffolds containing Nigella sativa herbal extract for effective wound healing

Sharifi

Bahrami

Nejad

et al. 2020

J of Applied Polymer Sci

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“…One of the problems in the field of tissue engineering that is not solved yet is to develop scaffolds that mimic the architecture of tissue at the nanoscale. Bone tissue consists of different types of bone cells (osteoblasts, osteocytes, and osteoclasts) and a mineralization matrix formed by collagen nanofibers and nano-hydroxyapatite (nano-HA) [2]. Since the nanofiber structure is similar to the extracellular matrix, which is an important controller of cell adhesion, proliferation, migration, and differentiation, nanofiber is a promising structural element for scaffold fabrication [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds

Permyakova

Kiryukhantsev-Korneev

Gudz

et al. 2019

Nanomaterials

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Due to their good mechanical stability compared to gelatin, collagen or polyethylene glycol nanofibers and slow degradation rate, biodegradable poly-ε-caprolactone (PCL) nanofibers are promising material as scaffolds for bone and soft-tissue engineering. Here, PCL nanofibers were prepared by the electrospinning method and then subjected to surface functionalization aimed at improving their biocompatibility and bioactivity. For surface modification, two approaches were used: (i) COOH-containing polymer was deposited on the PCL surface using atmospheric pressure plasma copolymerization of CO 2 and C 2 H 4 , and (ii) PCL nanofibers were coated with multifunctional bioactive nanostructured TiCaPCON film by magnetron sputtering of TiC-CaO-Ti 3 PO x target. To evaluate bone regeneration ability in vitro, the surface-modified PCL nanofibers were immersed in simulated body fluid (SBF, 1×) for 21 days. The results obtained indicate different osteoblastic and epithelial cell response depending on the modification method. The TiCaPCON-coated PCL nanofibers exhibited enhanced adhesion and proliferation of MC3T3-E1 cells, promoted the formation of Ca-based mineralized layer in SBF and, therefore, can be considered as promising material for bone tissue regeneration. The PCL-COOH nanofibers demonstrated improved adhesion and proliferation of IAR-2 cells, which shows their high potential for skin reparation and wound dressing.

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“…Various modification strategies to increase the cell adhesion of PLA have been reported, such as copolymerizing with other polymers (Jiao & Cui, ), plasma treatment (Khorasani, Mirzadeh, & Irani, ), chemical modification (Ma, Gao, Ji, & Shen, ), and physical adsorption (Kakinoki & Yamaoka, ). PLA nanofibers have gained attention because they can be fabricated to resemble the extracellular matrix; the cell adhesion area can also be improved by decreasing poly( l ‐lactic acid) PLLA fiber diameter (Jang, Castano, & Kim, ; Mo, Xu, Kotaki, & Ramakrishna, ; Parvathi, Krishnan, Anitha, Jayakumar, & Manitha, ). However, these modification strategies, which use plasma or solvent, may destroy the nanofiber structure.…”

Section: Introductionmentioning

confidence: 99%

Improved exposure of bioactive peptides to the outermost surface of the polylactic acid nanofiber scaffold

Hsu

Yamaoka

2019

J Biomed Mater Res

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We recently reported a novel method to modify the polylactic acid (PLLA) nanofiber scaffold with IKVAV peptide/oligo (d‐lactic acid) (ODLA) heteroconjugates. However, a part of the IKVAV sequence was not exposed to the outermost surface and therefore, cell adhesion was not optimized. In this study, we evaluated two strategies to improve the exposing efficiency of IKVAV. One strategy introduced a hydrophilic diethylene glycol (diEG) segment between IKVAV and ODLA. In the second method, these modified nanofibers were heated to a given temperature and cooled to room temperature in water. The IKVAV peptides at the outermost surface were quantified by the fluorescein isothiocyanate staining method. After being heated above the PLLA glass transition temperature, the exposing efficiency of the IKVAV sequence on the PLLA/ODLA–diEG–IKVAV nanofiber was three times higher than that on the PLLA/ODLA–IKVAV unheated nanofiber. Moreover, nearly 100% of the PC‐12 cells seeded onto the PLLA/ODLA–diEG–IKVAV nanofiber and were well distributed, while only 50% of the PC‐12 cells seeded onto the PLLA and PLLA/ODLA–IKVAV nanofibers. These strategies markedly enhanced the exposing efficiency of the bioactive peptides; therefore, their use can be applicable to other nanofiber scaffolds.

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Poly(L-lactic acid) nanofibers containing Cissus quadrangularis induced osteogenic differentiation in vitro

Cited by 30 publications

References 35 publications

Electrospun PCL and PLA hybrid nanofibrous scaffolds containing Nigella sativa herbal extract for effective wound healing

Electrospun PCL and PLA hybrid nanofibrous scaffolds containing Nigella sativa herbal extract for effective wound healing

Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds

Improved exposure of bioactive peptides to the outermost surface of the polylactic acid nanofiber scaffold

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