Electrospun PCL and PLA hybrid nanofibrous scaffolds containing Nigella sativa herbal extract for effective wound healing

Abstract

Black seed (Nigella sativa [NS]) is used in traditional medicine as an antibacterial agent. In this study, novel hybrid scaffolds were manufactured from poly(ɛ‐caprolactone[PCL])/ Poly(lactic acid [PLA]) with NS extract by double‐nozzle electrospinning for wound healing application. Optimal conditions were found using response surface methodology including feed rate 0.8 ml/hr, voltage 20 kV and PLA 8% /PCL 10% concentrations. The effect of NS extract on the properties of the scaffold was evaluated by scanning electron microscopy, contact angle, and mechanical test. The PLA‐PCL/NS 20% beadles, and smooth nanofibrous web was obtained as the optimum scaffold with an average diameter of 638 ± 69 nm and the contact angle of 44°. In addition, the biological properties of the scaffolds such as antibacterial against Staphylococcus aureus (gram‐positive) and Escherichia coli (gram‐negative) bacteria, MTT assay, extract release, and cell growth (human mesenchymal stem cells) were examined. Incorporation of NS extract into the nanofibers caused to enhance the biological properties, cell viability and cell proliferation without toxicity.

Electrospun polylactic acid/sulfadiazine sodium/proteinase nanofibers and their applications in treating frostbite

“…Some articles have also proved that electrospun polylactic acid material also has a certain antibacterial effect. 47,48 There are few reports on the preparation of nano materials containing sulfadiazine sodium by electros pun fibers. In this study, the effects of different components of proteinase K/polylactic acid electros spun fibers containing sulfadiazine sodium on the release effect of drugs were evaluated, and the physical and angiogenic properties of sulfadiazine loaded nano fibers were characterized.…”

Nature-Derived and Synthetic Additives to poly(ɛ-Caprolactone) Nanofibrous Systems for Biomedicine; an Updated Overview

“…To address this shortcoming and to improve wettability thus cell interactivity of PCL nanofibers, they have been either surface coated/blended with hydrophilic natural polymers, e.g., collagen (Col), gelatine (Gel), chitosan (CS), etc., or incorporated with polar inorganic nanofillers made of, e.g., Ag and TiO 2 and bio-derived compounds, e.g., lawsone , Nigella Sativa, etc . ( Wang H. et al, 2017 ; Ghosal et al, 2017 ; Ren et al, 2017 ; Adeli-Sardou et al, 2019 ; Sharifi et al, 2020 ). In this regard, recently, Li et al (2021) developed a Col/PCL nanofiber wound dressing with superior wettability and mechanical stability.…”

An In Vitro Study of Antibacterial Properties of Electrospun Hypericum perforatum Oil-Loaded Poly(lactic Acid) Nonwovens for Potential Biomedical Applications

“…Researchers have been worked about the development antibacterial PLA-based electrospun materials using chitosan and silver [43], thymol [44], propolis [45], silver and vitamin E [46], copaiba (Copaifera sp.) oil [47], tetracycline hydrochloride and chitosan [48], cinnamon essential oil [49], thymoquinone [50], tea tree and manuka oil [51], carvacrol [52], clary sage and black pepper [53], terpinen-4-ol [54], epidermal growth factor [55], quercetin [56], Nigella sativa herbal extract [57], Eucalyptus essential oil [58], silver diclofenac complex [59], chitosan, black pepper essential oil and limonene [60], selenium and clarithromycin [61], cefazolin [62], silver nanoparticles [63], zinc oxide [64], thyme essential oil [65], zenian (Carum copticum) essential oil [66], birch bark triterpene extract [67], Capparis spinosa L. extracts [68], Plumbago europaea plant extract [69], curcumin [70], babassu oil [71], and cypress (Cupressus sempervirens L.) essential oil [72].…”