Poly-(L-Lactic) Acid Membranes in Palatal Surgery in Beagle Dogs: Clinical and Histologic Evaluation

Braekt, Marcel M. H. In de; Maltha, Jaap C.; Kuijpers-Jagtman, A.M.

doi:10.1597/1545-1569_1995_032_0290_pllami_2.3.co_2

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…Two studies on biodegradable polymers implanted in the palate of dogs lack extensive description of the inflammatory response. 35,36 Both studies report the formation of a cellular capsule around the polymers, which points toward an extensive foreign-body reaction. Premature sequestration of the polymers also occurred.…”

Section: Discussionmentioning

confidence: 99%

Collagen Scaffolds Implanted in the Palatal Mucosa

Jansen

Kuijpers-Jagtman²,

Kuppevelt³

et al. 2008

Journal of Craniofacial Surgery

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Scar formation after repair of the cleft palate leads to growth impairment of the upper jaw and midface. The implantation of a suitable scaffold during surgery may reduce this adverse effect. However, little is known about tissue reactions to scaffolds implanted in the oral cavity. Our goal was to analyze the tissue reactions to cross-linked type I collagen scaffolds after submucoperiosteal implantation in the palate of rats. Collagen type I scaffolds were implanted in the palate of 25 male Wistar rats. Groups of 5 rats were killed consecutively after 1, 2, 4, 8, and 16 weeks and were processed for histologic and immunohistochemical analyses. After 1 and 2 weeks, 3 rats from the sham group were also killed. On hematoxylin and eosin-stained sections, the cell density and the number of giant cells were determined. Blood vessels, inflammation, and the presence of myofibroblasts were detected by immunohistochemistry. An influx of inflammatory cells started after 1 week but had completely subsided after 8 weeks. Myofibroblasts were observed within the scaffolds only in the first 2 weeks. Angiogenesis already started after 1 week and showed a peak after 4 weeks, slowly declining afterward. The scaffolds were gradually integrated within the host tissue and only elicited a mild and transient inflammatory response. The scaffolds were biocompatible and seemed to be promising for future applications in cleft palate surgery.

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Section: Discussionmentioning

confidence: 99%

Collagen Scaffolds Implanted in the Palatal Mucosa

Jansen

Kuijpers-Jagtman²,

Kuppevelt³

et al. 2008

Journal of Craniofacial Surgery

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“…This does not conform to the outcome of several other studies, where earlier fracture is reported. In de Braekt et al [8,9] reported fracture of porous PLLA films (My = 220 kg/mol, thickness 110 lam, porosity range 0.5 ~m to 1.0 ~m) into several small parts starting after the first week of implantation on the palate of Beagle dogs. Galgut et al [-13] reported fracture of PLLA membranes {Mv-50kg/mol and Mv = 200kg/mol, thickness 20 lain, 40 jam and 70 jam) and of membranes made of a copolymer of PHB-co HV 80/20 within 2 weeks after placement transcutaneously in the dorsum of rats.…”

Section: Discussionmentioning

confidence: 99%

“…The viscosity average molecular weights (My) were determined by viscometry and calculated by applying the Mark Houwink relationship [1][2][3][4][5][6][7][8][9][10][11][12]. For specifications of the materials given by the manufacturers see Table I.…”

Section: Methodsmentioning

confidence: 99%

“…This phenomenon might be responsible for the disturbance of dento-alveolar development [-6, 7]. Follow-up studies attempted to prevent the attachment of such scar tissue to the bone by submucoperiosteal insertion of porous poly-(L-lactic) acid (=PLLA) films (Mv = 220 kg/mol)_ Although it appeared that premature degradation of the films took place in vivo, the results were promising as no attachment of scar tissue to the bone was found in the vicinity of remnants of the films [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of non-porous films after submucoperiosteal implantation on the palate of Beagle dogs

Leenstra

Maltha

Kuijpers-Jagtman

1995

J Mater Sci: Mater Med

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The aim of this study is to investigate the in vivo behaviour of five different biodegradable films after submucoperiosteal implantation on the palate of Beagle dogs. Non-porous films of poly-(L-lactic) acid (= PLLA), high molecular weight poly-(L-lactic) acid (= HMW-PLLA), poly-(DL-lactic) acid (= PDLLA), poly(~-caprolactone) (= PCL) and a copolymer of poly hydroxybutyrate 80% hydroxyvalerate 20% (= PHB-co-HV 80/20) were implanted submucoperiostally on the palate of Beagle dogs. After 2, 4, 8 and 12 weeks in situ, the structure of the films and the tissue reactions were studied histologically. In terms of mechanical properties and tissue response, the PHB-co-HV film is the most suitable for use on dogs.

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“…In follow‐up studies, attempts were made to prevent the attachment of scar tissue by modifying the surgical techniques or by the use of biodegradable membranes. However, both approaches did not produce optimal long‐term results 36,37 …”

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confidence: 95%

Histologic evaluation of skin‐derived and collagen‐based substrates implanted in palatal wounds

Ophof

Maltha

Hoff

et al. 2004

Wound Repair Regeneration

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Tissue shortage complicates the surgery of cleft lip and palate anomalies and the healing of defects on the palate impairs growth of the dento-alveolar complex due to scar tissue formation. Implantation of substitutes into the wound area might overcome this adverse effect. The aim of this study was to compare the tissue response to three collagen-based (collagen type I substrate alone, or collagen coated with elastin or chondroitin-6-sulfate) and two skin-derived substrates (unprocessed dermis and AlloDerm) after implantation into 12 dogs. Histology was performed at 3, 10, and 20 days postsurgery. We showed that all substrates were well tolerated. However, it is unclear whether AlloDerm was rapidly degraded or if it was sequestrated. There was no elastin or collagen present in these wounds. All collagen-based substrates showed good epithelial regeneration, although heparan sulfate (JM 403) was absent. Wounds treated with the collagen-based substrates contained fewer myofibroblasts at 20 days postsurgery and the type III collagen fibers in the immature scar tissue were more randomly oriented than in an untreated wound. In conclusion, palatal wounds with a dermal substrate heal with fewer indications of scar tissue formation and evoke only a mild inflammatory reaction, which is preferred over the tissue reaction in an untreated wound.

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Poly-(L-Lactic) Acid Membranes in Palatal Surgery in Beagle Dogs: Clinical and Histologic Evaluation

Cited by 6 publications

References 21 publications

Collagen Scaffolds Implanted in the Palatal Mucosa

Collagen Scaffolds Implanted in the Palatal Mucosa

Biodegradation of non-porous films after submucoperiosteal implantation on the palate of Beagle dogs

Histologic evaluation of skin‐derived and collagen‐based substrates implanted in palatal wounds

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