Biodegradation of non-porous films after submucoperiosteal implantation on the palate of Beagle dogs

Leenstra, Thomas S.; Maltha, Jaap C.; Kuijpers-Jagtman, Anne Marie

doi:10.1007/bf00123368

Cited by 10 publications

(5 citation statements)

References 13 publications

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“…In accordance with the literature, the presence of PLGA (50:50) resulted in a significant weight loss and a drop in pH of the PBS media 57–61. The degradation rate of PHBV8 is known to be very slow both in vivo and in vitro 62–67. It is evident that the PLGA component degrades and erodes from the film matrix (Fig.…”

Section: Discussionsupporting

confidence: 87%

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In vitro characterization of micropatterned PLGA‐PHBV8 blend films as temporary scaffolds for photoreceptor cells

Tezcaner¹

2007

J Biomedical Materials Res

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In developed countries the aging population faces increasing risks of blinding retinal diseases, for which there are few effective treatments available. Photoreceptor transplantation represents one approach, but generally results have been disappointing. We hypothesize that micropatterned biodegradable poly(L-lactic acid-co-glycolic acid)/poly(hydroxybutyrate-co-hydroxyvaleric acid) (PLGA-PHBV8) blend films could deliver photoreceptor cells in a more organized manner than bolus injections. Blending of PLGA and PHBV8 was used to optimize the degradation rate of the temporary template. At the end of 8 weeks, for both thin and thick films of PLGA-PHBV8 a 50% decrease of their initial weight with increasing water uptake was observed. When photoreceptor cells were seeded onto micropatterned PLGA-PHBV8 films with parallel grooves (21- and 42-microm-wide grooves and 20 microm ridge width and depth), the cells preferred laminin-deposited grooves to ridges and expressed rod- and cone-specific markers such as rhodopsin and arrestin. A loss in photoreceptor viability of 50% was observed after 7 days in culture. The effects of either retinal pigment epithelium (RPE)-derived or Muller glial cell-derived conditioned media or bFGF on the survival of photoreceptor cells seeded on PLGA-PHBV8 films were investigated. Addition of either RPE- and Muller-conditioned media increased statistically (p < 0.01) the viability of photoreceptor cells after 7 days of incubation. Our results suggest that such biodegradable micropatterned PLGA-PHBV8 blend films have a potential to deliver photoreceptor cells to the subretinal space and ensure laminar organization and maintenance of differentiation, and that incorporation of intrinsic factors within the scaffold would enhance the survival rate of transplanted cells.

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Section: Discussionsupporting

confidence: 87%

“…[57][58][59][60][61] The degradation rate of PHBV8 is known to be very slow both in vivo and in vitro. [62][63][64][65][66][67] It is evident that the PLGA component degrades and erodes from the film matrix (Fig. 2).The decrease of pH of the incubation media of PLGA-PHBV8 films was not as low as observed for PLGA films in the literature.…”

Section: Discussionmentioning

confidence: 92%

In vitro characterization of micropatterned PLGA‐PHBV8 blend films as temporary scaffolds for photoreceptor cells

Tezcaner¹

2007

J Biomedical Materials Res

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“…Although some portions of the scaffolds were found to have collapsed during sample preparation (mostly in pure PCL scaffolds, presumably due to its mechanical and thermal weaknesses), cell and tissue responses within the channels and surrounding the scaffold materials were clearly apparent. In the pure PCL scaffolds, a thick layer of fibrous tissue lined the scaffold stem surface, as well as a large number of fibroblasts was found, which has been typically observed in the biopolymer scaffolds like PCL 45, 46. Some new blood vessels were also formed (arrows).…”

Section: Resultsmentioning

confidence: 85%

“…In the pure PCL scaffolds, a thick layer of fibrous tissue lined the scaffold stem surface, as well as a large number of fibroblasts was found, which has been typically observed in the biopolymer scaffolds like PCL. 45,46 Some new blood vessels were also formed (arrows). Scaffold shape was better maintained in the PCL-HA and PCL-HA-imCNT scaffolds, revealing the pore configuration more easily.…”

Section: Biological Propertiesmentioning

confidence: 99%

Robocasting nanocomposite scaffolds of poly(caprolactone)/hydroxyapatite incorporating modified carbon nanotubes for hard tissue reconstruction

Dorj

Won

Kim

et al. 2012

J Biomedical Materials Res

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Nanocomposite scaffolds with tailored 3D pore configuration are promising candidates for the reconstruction of bone. Here we fabricated novel nanocomposite bone scaffolds through robocasting. Poly(caprolactone) (PCL)-hydroxyapatite (HA) slurry containing ionically modified carbon nanotubes (imCNTs) was robotic-dispensed and structured layer-by-layer into macrochanneled 3D scaffolds under adjusted processing conditions. Homogeneous dispersion of imCNTs (0.2 wt % relative to PCL-HA) was achieved in acetone, aiding in the preparation of PCL-HA-imCNTs slurry with good mixing property. Incorporation of imCNTs into PCL-HA composition significantly improved the compressive strength and elastic modulus of the robotic-dispensed scaffolds (~1.5-fold in strength and ~2.5-fold in elastic modulus). When incubated in simulated body fluid (SBF), PCL-HA-imCNT nanocomposite scaffold induced substantial mineralization of apatite in a similar manner to the PCL-HA scaffold, which was contrasted in pure PCL scaffold. MC3T3-E1 cell culture on the scaffolds demonstrated that cell proliferation levels were significantly higher in both PCL-HA-imCNT and PCL-HA than in pure PCL, and no significant difference was found between the nanocomposite scaffolds. When the PCL-HA-imCNT scaffold was implanted into a rat subcutaneous tissue for 4 weeks, soft fibrous tissues with neo-blood vessels formed well in the pore channels of the scaffolds without any significant inflammatory signs. Tissue reactions in PCL-HA-imCNT scaffold were similar to those in PCL-HA scaffold, suggesting incorporated imCNT did not negate the beneficial biological roles of HA. While more long-term in vivo research in bone defect models is needed to confirm clinical availability, our results suggest robotic-dispensed PCL-HA-imCNT nanocomposite scaffolds can be considered promising new candidate matrices for bone regeneration.

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“…Leenstra et al investigated the use of nonporous PHBV films to keep the mucoperiosteum and bone separated until the completion of the transition of teeth (at about 24 weeks). Compared to PLA or PCL, PHBV films were more suitable for this procedure in terms of mechanical properties and tissue response [180].…”

Section: Polyestersmentioning

confidence: 99%

Biodegradable polymers for dental tissue engineering and regeneration

Conte¹,

Riccitiello²,

Luise³

et al. 2017

Biodegradable Polymers: Recent Developments and New Perspectives

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Biodegradation of non-porous films after submucoperiosteal implantation on the palate of Beagle dogs

Cited by 10 publications

References 13 publications

In vitro characterization of micropatterned PLGA‐PHBV8 blend films as temporary scaffolds for photoreceptor cells

In vitro characterization of micropatterned PLGA‐PHBV8 blend films as temporary scaffolds for photoreceptor cells

Robocasting nanocomposite scaffolds of poly(caprolactone)/hydroxyapatite incorporating modified carbon nanotubes for hard tissue reconstruction

Biodegradable polymers for dental tissue engineering and regeneration

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