Poly-l-arginine modifications alter the organization and secretion of collagen in SKH1-E mice

Boddupalli, Anuraag; Akilbekova, Dana; Bratlie, Kaitlin M.

doi:10.1016/j.msec.2019.110143

Cited by 5 publications

(3 citation statements)

References 63 publications

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“…Collagen, which is one of the basic structural components of human skin [56] and accelerates fibroblast proliferation and endothelial migration [57,58], is used in many tissue engineering applications. However, immunogenic reactions may occur in humans due to animal-derived collagen use.…”

Section: Characterization Of Bovine Tendon Atelocollagenmentioning

confidence: 99%

An in vitro 3D diabetic human skin model from diabetic primary cells

et al. 2020

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Diabetes mellitus, a complex metabolic disorder, leads to many health complications like kidney failure, diabetic heart disease, stroke, and foot ulcers. Treatment approaches of diabetes and identification of the mechanisms underlying diabetic complications of the skin have gained importance due to continued rapid increase in the diabetes incidence. A thick and pre-vascularized in vitro 3D type 2 diabetic human skin model (DHSM) was developed in this study. The methacrylated gelatin (GelMA) hydrogel was produced by photocrosslinking and its pore size (54.85 ± 8.58 μm), compressive modulus (4.53 ± 0.67 kPa) and swelling ratio (17.5 ± 2.2%) were found to be suitable for skin tissue engineering. 8% GelMA hydrogel effectively supported the viability, spreading and proliferation of human dermal fibroblasts. By isolating dermal fibroblasts, human umbilical vein endothelial cells and keratinocytes from type 2 diabetic patients, an in vitro 3D type 2 DHSM, 12 mm in width and 1.86 mm thick, was constructed. The skin model consisted of a continuous basal epidermal layer and a dermal layer with blood capillary-like structures, ideal for evaluating the effects of anti-diabetic drugs and wound healing materials and factors. The functionality of the DHSM was showed by applying a therapeutic hydrogel into its central wound; especially fibroblast migration to the wound site was evident in 9 d. We have demonstrated that DHSM is a biologically relevant model with sensitivity and predictability in evaluating the diabetic wound healing potential of a therapeutic material.

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Section: Characterization Of Bovine Tendon Atelocollagenmentioning

confidence: 99%

An in vitro 3D diabetic human skin model from diabetic primary cells

et al. 2020

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“…Poly-L-Arg has a well-known function for the regeneration of damaged tissues in two main ways; First, arginase leads to proline production, which is a vital amino acid for promoting collagen synthesis. Second, the oxidative pathway controls the nitric oxide (NO) production, which is important for enhancing epithelialization, angiogenesis, and immune response activities [ 23 , 24 ]. Bygd et al [ 22 ] studied the biological ability of Poly-L-Arg for adjusting fibroblast cell responses.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and pH-sensitive methacrylate poly-L-Arginine/poly (β-amino ester) polymer for soft tissue engineering

Heydari

Varshosaz

Kharaziha

et al. 2023

J Mater Sci: Mater Med

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During the last decade, pH-sensitive biomaterials containing antibacterial agents have grown exponentially in soft tissue engineering. The aim of this study is to synthesize a biodegradable pH sensitive and antibacterial hydrogel with adjustable mechanical and physical properties for soft tissue engineering. This biodegradable copolymer hydrogel was made of Poly-L-Arginine methacrylate (Poly-L-ArgMA) and different poly (β- amino ester) (PβAE) polymers. PβAE was prepared with four different diacrylate/diamine monomers including; 1.1:1 (PβAE1), 1.5:1 (PβAE1.5), 2:1 (PβAE2), and 3:1 (PβAE3), which was UV cross-linked using dimethoxy phenyl-acetophenone agent. These PβAE were then used for preparation of Poly-L-ArgMA/PβAE polymers and revealed a tunable swelling ratio, depending on the pH conditions. Noticeably, the swelling ratio increased by 1.5 times when the pH decreased from 7.4 to 5.6 in the Poly-L-ArgMA/PβAE1.5 sample. Also, the controllable degradation rate and different mechanical properties were obtained, depending on the PβAE monomer ratio. Noticeably, the tensile strength of the PβAE hydrogel increased from 0.10 ± 0.04 MPa to 2.42 ± 0.3 MPa, when the acrylate/diamine monomer molar ratio increased from 1.1:1 to 3:1. In addition, Poly-L-ArgMA/PβAE samples significantly improved L929 cell viability, attachment and proliferation. Poly-L-ArgMA also enhanced the antibacterial activities of PβAE against both Escherichia coli (~5.1 times) and Staphylococcus aureus (~2.7 times). In summary, the antibacterial and pH-sensitive Poly-L-ArgMA/PβAE1.5 with suitable mechanical, degradation and biological properties could be an appropriate candidate for soft tissue engineering, specifically wound healing applications. Graphical Abstract

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“…The architecture, topology and mechanical properties of additively manufactured (AM) meta-biomaterials are still under intensive investigation [1]. The new scaffold design and its surface bioactivity enhancement still need improvement [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of electrochemically bioactivated Ti6Al4V 3D porous scaffolds

Myakinin

Turlybekuly

Pogrebnjak

et al. 2021

Materials Science and Engineering: C

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Triply periodic minimal surfaces (TPMS) are known for their advanced mechanical properties and are wrinkle-free with a smooth local topology. These surfaces provide suitable conditions for cell attachment and proliferation. In this study, the in vitro osteoinductive and antibacterial properties of scaffolds with different minimal pore diameters and architectures were investigated. For the first time, scaffolds with TPMS architecture were treated electrochemically by plasma electrolytic oxidation (PEO) with and without silver nanoparticles (AgNPs) to enhance the surface bioactivity. It was found that the scaffold architecture had a greater impact on the osteoblast cell activity than the pore size. Through control of the architecture type, the collagen production by osteoblast cells increased by 18.9% and by 43.0% in the case of additional surface PEO bioactivation. The manufactured scaffolds demonstrated an extremely low quasi-elastic modulus (comparable with trabecular and cortical bone), which was 5 -10 times lower than that of bulk titanium (6.4-11.4 GPa vs 100-105 GPa). The AgNPs provided antibacterial properties against both gram-positive and gram-negative bacteria and had no significant impact on the osteoblast cell growth. Complex experimental results show the in vitro effectiveness of the PEO-modified TPMS architecture, which could positively impact the clinical applications of porous bioactive implants.

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Poly-l-arginine modifications alter the organization and secretion of collagen in SKH1-E mice

Cited by 5 publications

References 63 publications

An in vitro 3D diabetic human skin model from diabetic primary cells

An in vitro 3D diabetic human skin model from diabetic primary cells

Antibacterial and pH-sensitive methacrylate poly-L-Arginine/poly (β-amino ester) polymer for soft tissue engineering

In vitro evaluation of electrochemically bioactivated Ti6Al4V 3D porous scaffolds

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