Poly I-Mercapto Poly C: Antiviral, Anticellular, and Pharmacologic Effects

Vastola, Kathleen; Ho, Yau-Kwan; Bardos, Thomas J.; Grossmayer, B J; Fruck-Diviak, L.; O’mallory, J.A.

doi:10.1016/s0022-5347(17)49174-9

Cited by 4 publications

(4 citation statements)

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“…Selective thiolation at the 5 position of the cytosine bases in polyI:polyC resulted in partially thiolated double-stranded RNA, polyI:MPC (Fig. 1 and IFNy (20)(21)(22)(23)(24)(25)(26)(27)(28)(29). It is of minimal toxicity in vivo in mice, rabbits, and guinea pigs and is subject to less degradation by plasma ribonucleases than its parent compound (22).…”

Section: Studies Of Double-stranded Rna Derivativesmentioning

confidence: 99%

“…1 and IFNy (20)(21)(22)(23)(24)(25)(26)(27)(28)(29). It is of minimal toxicity in vivo in mice, rabbits, and guinea pigs and is subject to less degradation by plasma ribonucleases than its parent compound (22). PolyI:MPC has significant antitemplate activity against DNA and RNA polymerases, including reverse transcriptase (which is of great significance in the treatment of human immunodeficiency virus [HIV] infection) (23)(24)(25)(26)(27).…”

Section: Studies Of Double-stranded Rna Derivativesmentioning

confidence: 99%

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Treatment of Viral and Neoplastic Diseases with Double-Stranded RNA Derivatives and Other New Agents

Ambrus

Chadha

Islam

et al. 2006

Exp Biol Med (Maywood)

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Many attempts have been made to inhibit viral and neoplastic diseases by targeting the RNA system. The pathophysiologic significance of the microRNA system and the therapeutic potential of its manipulation are discussed. Studies of double-stranded RNA derivatives are reviewed. The therapeutic potential of one of these compounds, polyI:MPC, is emphasized. Studies of other related antiviral and antineoplastic agents are discussed, including 2'-deoxyoligocytidilates and telomerase inhibitors.

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Section: Studies Of Double-stranded Rna Derivativesmentioning

confidence: 99%

Section: Studies Of Double-stranded Rna Derivativesmentioning

confidence: 99%

Treatment of Viral and Neoplastic Diseases with Double-Stranded RNA Derivatives and Other New Agents

Ambrus

Chadha

Islam

et al. 2006

Exp Biol Med (Maywood)

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“…This analogue, resulting from the partial C5-thiolation of cytosine, has been shown to induce interferon production to a greater extent than poly I:C and to have induction capabilities increased with increasing thiolation (O'Malley et al, 1975). Further work has established that poly I:MPC is less toxic in vivo than poly I:C (Vastola et al, 1984).…”

Section: Interferon Inductionmentioning

confidence: 99%

Oligonucleotides and Polyribonucleotides: A Review of Antiviral Activity

Markosian

Hyde

2005

Antivir Chem Chemother

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Current antiviral therapies are insufficient for treating emerging, re-emerging and established viral diseases. In an effort to find new therapeutics, oligo- and polyribonucleotides are being studied for their antiviral capabilities. Studies have shown that uniquely modified single- and double-stranded nucleic acid constructs are effective in inhibiting viral proliferation by various mechanisms. This review gives a brief history and highlights the development of oligo- and polyribonucleotides as antiviral agents primarily in the fields of interferon induction, mRNA complementation and reverse transcriptase inhibition.

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“…The National Cancer Institute anticancer drug screening program has recently discontinued use of this type of metabolic assay in favor of the histologic stain sulforhodamine B [9] Neutral red is a vital stain which accumulates in the lysosomal compartment following uptake via non-ionic diffusion [10,11]. Its use to quantitate viable cell numbers has been previously described [12][13][14]. The use of a 3T3 cell, neutral red based assay to assess the cytotoxicity of potential toxicants, in place of animal testing, was recently proposed [15].…”

Section: Introductionmentioning

confidence: 99%

A semi-automated neutral red based chemosensitivity assay for drug screening

et al. 1990

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A semi-automated colorimetric chemosensitivity assay was developed. The assay utilizes the vital stain neutral red for the rapid screening of potential anticancer agents using solid tumor cell lines. The cell lines used in this assay and presented in this report are CX-1 colon adenocarcinoma and A549 lung carcinoma. The assay, performed in 96 well tissue culture plates, allows for short drug exposure times (3 hrs.) followed by quantitation of cell number (neutral red absorbance) following four cell doubling times. Cell number directly correlated with absorbance of eluted neutral red at 540 nm. However, optimal amounts of dye and staining times varied between cell lines. IC50 concentrations (for inhibition of cell growth) determined using this assay were in good agreement with results from clonogenic assays using similar drug treatment conditions. The assay technique was determined to be capable of detecting antineoplastic compounds operating by a wide variety of mechanisms.

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Poly I-Mercapto Poly C: Antiviral, Anticellular, and Pharmacologic Effects

Cited by 4 publications

References 0 publications

Treatment of Viral and Neoplastic Diseases with Double-Stranded RNA Derivatives and Other New Agents

Treatment of Viral and Neoplastic Diseases with Double-Stranded RNA Derivatives and Other New Agents

Oligonucleotides and Polyribonucleotides: A Review of Antiviral Activity

A semi-automated neutral red based chemosensitivity assay for drug screening

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