Poly(ethylene glycol) Grafting to Poly(ether imide) Membranes: Influence on Protein Adsorption and Thrombocyte Adhesion

Neffe, Axel T.; Ruesten-Lange, Maik von; Braune, S.; Luetzow, K.; Roch, Toralf; Richau, K.; Jung, F.; Lendlein, Andreas

doi:10.1002/mabi.201300309

Cited by 31 publications

(24 citation statements)

References 37 publications

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“…This means that surface wettability plays an important role in suppressing platelet adhesion. PEG increased its surface hydrophilicity and could inhibit the platelet adhesion . It was noteworthy that the platelet adhesion on PS‐REDV, PS‐CAG, and PS‐REDV/CAG surfaces had no significant increase compared with PS‐PEG, indicating that the peptide grafting did not affect platelet adhesion.…”

Section: Resultsmentioning

confidence: 97%

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Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Bai

Zhao

et al. 2018

Macromolecular Bioscience

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Artificial small‐caliber vascular grafts are still limited in clinical application because of thrombosis, restenosis, and occlusion. Herein, a small‐caliber vascular graft (diameter 2 mm) is fabricated from poly(ε‐caprolactone)‐b‐poly(isobutyl‐morpholine‐2,5‐dione) (PCL‐PIBMD) and silk fibroin (SF) by electrospinning technology and then biofunctionalized with low‐fouling poly(ethylene glycol) (PEG) and two cell‐adhesive peptide sequences (CREDVW and CAGW) with the purpose of enhancing antithrombogenic activity and endothelialization. The successful grafting of PEG and peptide sequences is confirmed by X‐ray photoelectron spectroscopy. The suitable surface wettability of the modified vascular graft is testified by water contact angle analysis. The surface hemocompatibility is verified by platelet adhesion assays and protein adsorption assays, and the results demonstrate that both platelet adhesion and protein adsorption on the biofunctionalized surface are significantly reduced. In vitro studies demonstrate that the biofunctionalized surface with suitable hydrophilicity and cell‐adhesive peptides can selectively promote the adhesion, spreading, and proliferation of human umbilical vein endothelial cells. More importantly, compared with control groups, this biofunctionalized small‐caliber vascular graft shows high long‐term patency and endothelialization after 10 weeks of implantation. The biofunctionalization with PEG and two cell‐adhesive peptide sequences is an effective method to improve the endothelialization and long‐term performance of synthetic vascular grafts.

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Section: Resultsmentioning

confidence: 97%

“…The introduction of PEG distinctly reduced the protein adsorption. Many researches have proved the ability of PEG to inhibit protein adsorption and platelet adhesion . PEG can also contribute well hemocompatibility.…”

Section: Resultsmentioning

confidence: 99%

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Bai

Zhao

et al. 2018

Macromolecular Bioscience

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“…Functionalization can be achieved, whereas the main polymer chain is kept intact. The membrane surface was functionalized by grafting PEGs,213 linear polyglycerols,51 heparin, polyethylenimine,214 and tris(hydroxymethyl)aminomethane 215. PEG and linear polyglycerols did not entirely prevent protein adsorption, and interestingly they had only a minor effect on thrombocyte adhesion and activation.…”

Section: Bioinert and Biospecific Surfaces—recent Developments Andmentioning

confidence: 99%

Protein Interactions with Polymer Coatings and Biomaterials

et al. 2014

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Protein adsorption is considered to be the most important factor of the interaction between polymeric biomaterials and body fluids or tissues. Water-mediated hydrophobic and hydration forces as well as electrostatic interactions are believed to be the major factors of protein adsorption. A systematic analysis of various monolayer systems has resulted in general guidelines, the so-called "Whitesides rules". These concepts have been successfully applied for designing various protein-resistant surfaces and are being studied to expand the understanding of protein-material interactions beyond existing limitations. Theories on the mechanisms of protein adsorption are constantly being improved due to the fast-developing analytical technologies. This Review is aimed at improving these empirical guidelines with regard to present theoretical and analytical advances. Current analytical methods to test mechanistic hypotheses and theories of protein-surface interactions will be discussed. Special focus will be given to state-of-the-art bioinert and biospecific coatings and their applications in biomedicine.

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“…Platelet aggregation indices (TAI) were obtained according to the method of Grotemeyer. 19,29,42 Platelet function was assessed with the DadeV R PFA-100 platelet function analyzer (Siemens Healthcare Diagnostics, Marburg, Germany). The reactivity of platelets in sodium citrate anticoagulated whole blood on equine type I collagen and epinephrine bitartrate as well as equine type I collagen and adenosine-5 0diphosphate 43,44 was tested.…”

Section: Study Populationmentioning

confidence: 99%

Adhesion and activation of platelets from subjects with coronary artery disease and apparently healthy individuals on biomaterials

et al. 2015

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On the basis of the clinical studies in patients with coronary artery disease (CAD) presenting an increased percentage of activated platelets, we hypothesized that hemocompatibility testing utilizing platelets from healthy individuals may result in an underestimation of the materials' thrombogenicity. Therefore, we investigated the interaction of polymer-based biomaterials with platelets from CAD patients in comparison to platelets from apparently healthy individuals. In vitro static thrombogenicity tests revealed that adherent platelet densities and total platelet covered areas were significantly increased for the low (polydimethylsiloxane, PDMS) and medium (Collagen) thrombogenic surfaces in the CAD group compared to the healthy subjects group. The area per single platelet-indicating the spreading and activation of the platelets-was markedly increased on PDMS treated with PRP from CAD subjects. This could not be observed for collagen or polytetrafluoroethylene (PTFE). For the latter material, platelet adhesion and surface coverage did not differ between the two groups. Irrespective of the substrate, the variability of these parameters was increased for CAD patients compared to healthy subjects. This indicates a higher reactivity of platelets from CAD patients compared to the healthy individuals. Our results revealed, for the first time, that utilizing platelets from apparently healthy donors bears the risk of underestimating the thrombogenicity of polymer-based biomaterials.

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Poly(ethylene glycol) Grafting to Poly(ether imide) Membranes: Influence on Protein Adsorption and Thrombocyte Adhesion

Cited by 31 publications

References 37 publications

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Protein Interactions with Polymer Coatings and Biomaterials

Adhesion and activation of platelets from subjects with coronary artery disease and apparently healthy individuals on biomaterials

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