Poly(dimethylsiloxane) (PDMS) surface patterning by biocompatible photo-crosslinking block copolymers

Kuroda, Keita; Miyoshi, Hiromi; Fujii, Shota; Hirai, Tomoyasu; Takahara, Atsushi; Nakao, Aiko; Iwasaki, Yasuhiko; Morigaki, Kenichi; Ishihara, Kazuhíko; Yusa, Shin Ichi

doi:10.1039/c5ra08843g

Cited by 12 publications

(10 citation statements)

References 47 publications

(48 reference statements)

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“…Cell–material interactions constitute important fundamental topics in the fields of biomedicine and biomaterials. − The surface topography and physicochemical environment influence the cellular behavior profoundly at the cell–substrate interface. , With the development of surface patterning techniques, − appropriate microscale and nanoscale features have been found to influence cell behavior without necessarily destroying the cellular biochemical environment. Topological controls of various cellular responses, including cell adhesion, proliferation, migration, and differentiation, have been reported in recent years. − The underlying mechanisms of the cellular responses to topological features have been proposed recently, such as the discovery of the key proteins in the membrane curvature. − …”

Section: Introductionmentioning

confidence: 99%

Proliferation of Cells with Severe Nuclear Deformation on a Micropillar Array

et al. 2018

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Cellular responses on a topographic surface are fundamental topics about interfaces and biology. Herein, a poly(lactide-co-glycolide) (PLGA) micropillar array was prepared and found to trigger significant self-deformation of cell nuclei. The time-dependent cell viability and thus cell proliferation was investigated. Despite significant nuclear deformation, all of the examined cell types (Hela, HepG2, MC3T3-E1, and NIH3T3) could survive and proliferate on the micropillar array yet exhibited different proliferation abilities. Compared to the corresponding groups on the smooth surface, the cell proliferation abilities on the micropillar array were decreased for Hela and MC3T3-E1 cells and did not change significantly for HepG2 and NIH3T3 cells. We also found that whether the proliferation ability changed was related to whether the nuclear sizes decreased in the micropillar array, and thus the size deformation of cell nuclei should, besides shape deformation, be taken into consideration in studies of cells on topological surfaces.

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Section: Introductionmentioning

confidence: 99%

Proliferation of Cells with Severe Nuclear Deformation on a Micropillar Array

et al. 2018

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“…Poly(dimethylsiloxane) (PDMS) precursor and crosslinker (SYLGARD 184, silicone elastomer kit) were obtained from Dow Corning (USA). The two components were thoroughly mixed at a 10:1 (w/w) ratio of precursor: crosslinker and then poured onto a glass Petri dish, degassed in a vacuum for 30 min and cured at 70 °C for 1 h to obtain the PDMS substrate . Polyethylene (PE), poly(methyl methacrylate) (PMMA), and polyether ether ketone (PEEK) were bought from Hitachi Chemical Co., Ltd. (Tokyo, Japan), Kuraray Co., Ltd. (Tokyo, Japan) and Röchling, Mannheim (Germany), respectively.…”

Section: Methodsmentioning

confidence: 99%

Clickable Zwitterionic Copolymer as a Universal Biofilm‐Resistant Coating

Sae‐ung

Wijitamornloet

Iwasaki

et al. 2019

Macro Materials & Eng

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Hospital‐acquired infections are often caused by bacterial biofilms on medical devices. To prevent biofilm formation, herein, a universal coating of an antifouling polymer that inhibits the initial adhesion of bacteria is developed. This copolymer is made of methacryloyloxyethyl phosphorylcholine (MPC) and a methacrylate‐substituted dihydrolipoic acid (DHLA) (poly(MPC‐DHLA)). The MPC units provide the antifouling property, while the DHLA units offer cross‐linkable sites via thiol‐ene reactions to form the stable coated copolymer film. Without the requirement for covalent surface grafting, the poly(MPC‐DHLA) coating on various biomedically relevant substrates is investigated, where X‐ray photoelectron spectroscopy, water contact angle measurements, atomic force microscopy, and ellipsometry are used to confirm the success of the surface coating. Moreover, to mimic an actual clinical use, the copolymer coating is applied on a titanium dental substrate and the ability to inhibit biofilm formation by Staphylococcus aureus is quantified and visualized by crystal violet staining and scanning electron microscopy, respectively. As compared with the bare substrate, an effective reduction in bacterial adhesion and suppression of the subsequent biofilm formation is observed on the copolymer‐modified substrate. These features are maintained for up to 7 d indicating the durability as well as universal applicability of this coating approach.

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“…Contact lenses are possible targets for terpolymer application because terpolymer cast films suppress protein adsorption and have light transmission in the visible wavelength. Kuroda et al prepared amphiphilic diblock copolymers (PMPC‐P(TSM/CEA)) composed of PMPC block and random copolymer block that contained TSM and 2‐cinnamoyloxyethyl acrylate (CEA), which has a photocrosslinkable cinnamoyl group. The PMPC‐P(TSM/CEA) film could be formed stably onto a PDMS substrate when exposed to ultraviolet irradiation as the molecular weight increases because of crosslinking reactions.…”

Section: Introductionmentioning

confidence: 99%

Interpolymer association of amphiphilic diblock copolymers bearing pendant siloxane and phosphorylcholine groups

Kozuka

Kuroda

Ishihara

et al. 2019

J. Polym. Sci. Part A: Polym. Chem.

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In this study, amphiphilic diblock copolymers (PMPC m -PTSM n ) composed of hydrophilic poly(2-methacryloylo xyethyl phosphorylcholine) (PMPC) and hydrophobic poly(3-(tris (trimethylsiloxy)silyl)propyl methacrylate) (PTSM) were prepared via RAFT-controlled radical polymerization. The subscripts m and n represent the degrees of polymerization of the PMPC and PTSM blocks, respectively. Thus, the m/n ratio represents the hydrophilic/hydrophobic balance of the block copolymer. We prepared PMPC m -PTSM n with m/n ratios of 12, 1.8, and 0.3 and studied their association behaviors in water. PMPC m -PTSM n with m/n ratios of 12 and 1.8 could dissolve in water. However, PMPC m -PTSM n with an m/n ratio of 0.3 did not directly dissolve in water. First, PMPC m -PTSM n with an m/n ratio of 0.3 dissolved in ethanol, whose solution was dialyzed in water to prepare the aqueous solution. The solubilizing state of these polymers in water was examined by light scattering, a fluorescence probe method, and TEM. These polymers took interpolymer aggregation state with various morphologies. The m/n ratios of 12, 1.8, and 0.3 yielded spherical shapes, cylindrical micelles, and vesicle interpolymer aggregate shapes, respectively.Polydimethylsiloxane (PDMS) is flexible and hydrophobic and is characterized by processability and low cytotoxicity. 7-10 Lowmolecular-weight siloxane surfactants are used to manufacture textiles, cosmetics, formulations, agricultural chemicals, paints, and other technologies. Amphiphilic diblock copolymers that contain siloxane groups, which act as a hydrophobic block, have been prepared in the previous studies. For example, diblock copolymers composed of hydrophilic PEO and PDMS 11,12 form spherical micelles, cylinders, and vesicles in water according to the hydrophilic/hydrophobic block chain length ratio, which is due to the hydrophobic interactions between siloxane blocks. Car et al. 13 prepared pH-responsive diblock copolymers composed of PDMS and poly(2-(dimethylamino)ethyl methacrylate) blocks and reported their pH-responsive behavior in an aqueous solution.Poly(2-(methacryloyloxy)ethyl phosphorylcholine) (PMPC) has pendant phosphorylcholine group, which is the same chemical structure as one of the polar parts of the phospholipids that constitutes the cell membrane. 14 PMPC is water-soluble polymer and the surfaces bound with the PMPC shows an excellent resistance for protein adsorption and cell adhesion. Seo et al. 15 reported the preparation of amphiphilic ABA triblock copolymers composed of PMPC as an "A" block and PDMS as a "B" block, as well as random copolymers composed of 2-(methacryloyloxy)ethyl phosphory lcholine (MPC) and 3-(tris(trimethylsiloxy)silyl)propyl methacrylate (TSM) units. ABA triblock copolymers could attach on the PDMS substrates, which are more stable than random copolymers. Since the PMPC unit located on the PDMS substrate surface, Additional supporting information may be found in the online version of this article.

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Poly(dimethylsiloxane) (PDMS) surface patterning by biocompatible photo-crosslinking block copolymers

Abstract: Poly(dimethylsiloxane) (PDMS) surface was patterned by poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)-containing photo-crosslinking diblock copolymers upon photo-irradiation.

Cited by 12 publications

References 47 publications

Proliferation of Cells with Severe Nuclear Deformation on a Micropillar Array

Proliferation of Cells with Severe Nuclear Deformation on a Micropillar Array

Clickable Zwitterionic Copolymer as a Universal Biofilm‐Resistant Coating

Interpolymer association of amphiphilic diblock copolymers bearing pendant siloxane and phosphorylcholine groups

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