Poly-d-lysine in G2 potentiates chromosome damage induced by X-rays and mitomycin C in CHO cells

Cortés, Felipe; Mateos, Santiago; Ortiz, T.; Panneerselvam, N.; Mateos, Juán Carlos Pachón

doi:10.1016/0027-5107(92)90177-4

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Also, some chemicals possess abilities to reduce the mutagenic effects caused by other chemicals [31][32][33][34][35]. A few articles describe synergy or exacerbation of response after combined treatment [36][37][38][39].…”

Section: Discussionmentioning

confidence: 99%

Induction of micronuclei in bone marrow and sperm head abnormalities after combined exposure of mice to low doses of X-rays and acrylamide

Dobrzyńska¹,

Gajewski²

2000

Teratog. Carcinog. Mutagen.

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Section: Discussionmentioning

confidence: 99%

Induction of micronuclei in bone marrow and sperm head abnormalities after combined exposure of mice to low doses of X-rays and acrylamide

Dobrzyńska¹,

Gajewski²

2000

Teratog. Carcinog. Mutagen.

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“…A total of ∼200 cells/dose were scored, and cells with 10 or more foci were scored as positive. Ionizing radiation was used as a positive control for the assay; cells were exposed to 4 Gy of ionizing radiation using an X-ray irradiator (Philips MU15F) operated at 100 kV and a dose rate of 1 Gy/min. , …”

Section: Methodsmentioning

confidence: 99%

“…Ionizing radiation was used as a positive control for the assay; cells were exposed to 4 Gy of ionizing radiation using an X-ray irradiator (Philips MU15F) operated at 100 kV and a dose rate of 1 Gy/min. 35,36 TARDIS Assay. This immunofluorescence technique employs specific antibodies to DNA topo I or topo II to detect the protein covalently bound to the DNA in individual cells.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

The Coffee Constituent Chlorogenic Acid Induces Cellular DNA Damage and Formation of Topoisomerase I– and II–DNA Complexes in Cells

Burgos-Morón

Calderón-Montaño

Orta

et al. 2012

J. Agric. Food Chem.

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Chlorogenic acid (CGA) is a plant polyphenol with known antioxidant properties. Although some studies suggest that CGA has anticancer properties, others indicate that this dietary constituent may cause DNA damage and induce carcinogenic effects. Because CGA is widely consumed in the form of coffee, it is important to further evaluate the putative DNA-damaging activity of CGA. Here we have employed two standard techniques commonly used for DNA damage detection (the comet assay and the γ- H2AX focus assay) and observed that CGA (0.5-5 mM) induces DNA damage in normal and cancer cells. We report for the first time that CGA induces high levels of topoisomerase I- and topoisomerase II-DNA complexes in cells (TARDIS assay). Catalase pretreatment abolished the formation of these topoisomerase-DNA complexes and reduced the cytotoxic activity of CGA, therefore indicating that hydrogen peroxide plays an important role in these activities. Lung cancer cells (A549) were more sensitive than normal lung fibroblasts (MRC5) to the cytotoxic activity of CGA, supporting previous findings that CGA may induce selective killing of cancer cells. Taking into consideration our results and the pharmacokinetic profile of CGA, the possible cancer preventive, carcinogenic and therapeutic potential of this dietary agent are discussed.

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Induction of micronuclei in mouse bone marrow after combined X‐rays‐cyclophosphamide and X‐rays‐mitomycin C treatments

Dobrzyńska¹,

Gajewski²

1999

Teratog. Carcinog. Mutagen.

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Poly-d-lysine in G2 potentiates chromosome damage induced by X-rays and mitomycin C in CHO cells

Cited by 4 publications

References 15 publications

Induction of micronuclei in bone marrow and sperm head abnormalities after combined exposure of mice to low doses of X-rays and acrylamide

Induction of micronuclei in bone marrow and sperm head abnormalities after combined exposure of mice to low doses of X-rays and acrylamide

The Coffee Constituent Chlorogenic Acid Induces Cellular DNA Damage and Formation of Topoisomerase I– and II–DNA Complexes in Cells

Induction of micronuclei in mouse bone marrow after combined X‐rays‐cyclophosphamide and X‐rays‐mitomycin C treatments

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