Poly (amino ester) Composed of Poly (ethylene glycol) and Aminosilane Prepared by Combinatorial Chemistry as a Gene Carrier

Abstract

Addition of PEGDA over APES resulted in a novel PAE which has high safety and transfection efficiency. Transfection and cytotoxicity are very sensitive to monomer ratios and mainly governed by concentration of amine monomer.

Review: Bioanalytical applications of biomolecule-functionalized nanometer-sized doped silica particles

“…Low-molecular-weight silica nanoscale colloidal particles with enhanced biocompatibility and an ordered porous structure were also shown to be effective when applied to the localized release of recombinant DNA [203]. Recently, a novel gene carrier, poly(amino ester) (PAE; composed of PEG and aminosilane) has been highlighted for its gene delivery efficiency and its safety towards 293T and HeLa cells [204]. PAEs, synthesized at different molar ratios with APTES and poly(ethylene glycol) diacrylate (PEGDA) by the Michael addition reaction, exhibited both high transfection efficiency and low cytotoxicity (as compared to polyethylenimine (25 KDa) and lipofectamine).…”

Inhaled Gene Therapy in Lung Cancer: “as for the future, Our Task is Not to Foresee it, But to Enable it”

“…Hydrolyzing enzymes can also be added to disrupt the mucosal network [14,15]. Glucosylated PEI and poly(b-amino ester) have been less toxic than PEI [11,16]. Tumor-suppressor gene strategies investigated include p53, Akt, BC-819, UAC/PDC4, PTEN and WT1-2 [17][18][19].…”

Discovery of Cationic Polymers for Non-Viral Gene Delivery Using Combinatorial Approaches

“…Six different polymer:DNA weight ratios were tested for each of these polymers for transgene expression efficacy in 293T and HeLa cells [45]. The polymers were synthesized using Michael’s addition reaction between amine-containing γ-aminopropyl-triethoxysilane (APES) and polyethylene glycol diacrylate (PEGDA; 258Da) at different mole ratios in which the APES was linked to PEGDA by hydrolysable ester linkages.…”