Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect

Волков, А. В.; Мураев, А. А.; Zharkova, I. I.; Voinova, V. V.; Akoulina, Elizaveta; Жуйков, В. А.; Khaydapova, D. D.; Chesnokova, Dariana V.; Menshikh, Ksenia A.; Дудун, А. А.; Махина, Т. К.; Бонарцева, Г. А.; Asfarov, T. F.; Stamboliev, I.А.; Gazhva, Yu.V.; Ryabova, Valentina; Zlatev, Lubomir H.; Ivanov, S.Yu.; Шайтан, К. В.; Бонарцев, А. П.

doi:10.1016/j.msec.2020.110991

Cited by 52 publications

(24 citation statements)

References 68 publications

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“…Many studies have demonstrated promising results for improvements in bone healing using cellular therapy associated with biomaterials (Tajima et al 2015;Venkatesan et al 2016;Volkov et al 2020). Our results provide strong evidence of the beneficial effects of MSC in bone neoformation in segmental defects in the distal radial diaphysis.…”

Section: Discussionsupporting

confidence: 66%

Autologous adipose-derived mesenchymal stem cells and hydroxyapatite for bone defect in rabbits

Franco¹,

Minto²,

Coelho³

et al. 2022

Vet. Med. - Czech

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This study aims to evaluate the effect of autologous adipose-derived mesenchymal stem cells (AAD-MSC), with and without synthetic absorbable hydroxyapatite (HAP-91), on the bone regeneration in rabbits. Thirty-four female white New Zealand rabbits were submitted to a 10 mm distal diaphyseal radius ostectomy, divided into 3 experimental groups according to the treatment established. The bone gap was filled with 0.15 ml of a 0.9% saline solution containing two million AAD-MSC (G1), or AAD-MSC associated with HAP-91 (G2). The control group (CG) received only 0.15 ml of the 0.9% saline solution. Radiographs were made post-operatively, and after 15, 30, 45 and 90 days. Fifty percent of the samples were submitted to a histological examination at 45 days and the remaining ones at 90 days post-operatively. Radiographically, the periosteal reaction, bone callus volume and bone bridge quality were superior in G2 (P < 0.05). Histologically, the bone repair was faster and more efficient in G1 at 45 days (P < 0.05). In conclusion, AAD-MSC improved the regeneration on the experimentally induced bone defects in rabbits; however, the use of hydroxyapatite requires caution given the granulomatous reaction produced in the species.

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Section: Discussionsupporting

confidence: 66%

Autologous adipose-derived mesenchymal stem cells and hydroxyapatite for bone defect in rabbits

Franco¹,

Minto²,

Coelho³

et al. 2022

Vet. Med. - Czech

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“…Bone tissue has regenerative capacity, but when a critical size defect occurs, regeneration becomes very difficult or even impossible. The regenerative capacity of tissues also decreases with human age [ 1 ]. Lack of bone regeneration does not only pose a serious health issue for the patient, but may also induce numerous physiological and economic problems related to long-term treatment [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

Krok-Borkowicz

Reczyńska

Rumian

et al. 2020

IJMS

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Poly(l-lactide-co-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leaching method. Their total porosity was 85% and the pore size was in the range of 250–320 µm. The physico-chemical properties of the scaffolds were evaluated using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), sessile drop, and compression tests. Three types of the scaffolds (unmodified PLGA, PLGA/coll, and PLGA/HAp) were implanted into the defects created in New Zealand rabbit femoral trochlears; empty defect acted as control. Samples were extracted after 1, 4, 12, and 26 weeks from the implantation, evaluated using micro-computed tomography (µCT), and stained by Masson–Goldner and hematoxylin-eosin. The results showed that the proposed method is suitable for fabrication of highly porous PLGA scaffolds. Effective deposition of both coll and HAp was confirmed on all surfaces of the pores through the entire scaffold volume. In the in vivo model, PLGA and PLGA/HAp scaffolds enhanced tissue ingrowth as shown by histological and morphometric analyses. Bone formation was the highest for PLGA/HAp scaffolds as evidenced by µCT. Neo-tissue formation in the defect site was well correlated with degradation kinetics of the scaffold material. Interestingly, around PLGA/coll extensive inflammation and inhibited tissue healing were detected, presumably due to immunological response of the host towards collagen of bovine origin. To summarize, PLGA scaffolds modified with HAp are the most promising materials for bone tissue regeneration.

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“…Meanwhile, an in vitro study using dual-doped hydroxyapatite coated with Ti-6AL-4 V has shown excellent biocompatibility towards the cell line [ 80 ]. A tomographical and histological study showed that the regeneration of critical-sized calvarial bone defects in vivo at the 28th day after an implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds is 3.6 times higher than the formation of bone tissue at 22–28 days, in comparison with acellular PHB/HA/ALG scaffolds [ 81 ]. Lima et al [ 82 ] reported an injectable substitute mixture of hydroxyapatite and beta-tricalcium phosphate exhibited no cytotoxicity and excellent results from in vivo, using tibia bone defects in rabbits at 30 and 60 days.…”

Section: Alginate Gelatine and Hydroxyapatite For Bone Tissue Rementioning

confidence: 99%

A Bibliometric Analysis of the Global Trend of Using Alginate, Gelatine, and Hydroxyapatite for Bone Tissue Regeneration Applications

et al. 2021

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Collecting information from previous investigations and expressing it in a scientometrics study can be a priceless guide to getting a complete overview of a specific research area. The aim of this study is to explore the interrelated connection between alginate, gelatine, and hydroxyapatite within the scope of bone tissue and scaffold. A review of traditional literature with data mining procedures using bibliometric analyses was considered to identify the evolution of the selected research area between 2009 and 2019. Bibliometric methods and knowledge visualization technologies were implemented to investigate diverse publications based on the following indicators: year of publication, document type, language, country, institution, author, journal, keyword, and number of citations. An analysis using a bibliometric study found that 7446 papers were located with the keywords “bone tissue” and “scaffold”, and 1767 (alginate), 185 (gelatine), 5658 (hydroxyapatite) papers with those specific sub keywords. The number of publications that relate to “tissue engineering” and bone more than doubled between 2009 (1352) and 2019 (2839). China, the United States and India are the most productive countries, while Sichuan University and the Chinese Academy of Science from China are the most important institutions related to bone tissue scaffold. Materials Science and Engineering C is the most productive journal, followed by the Journal of Biomedical Materials Research Part A. This paper is a starting point, providing the first bibliometric analysis study of bone tissue and scaffold considering alginate, gelatine and hydroxyapatite. A bibliometric analysis would greatly assist in giving a scientific insight to support desired future research work, not only associated with bone tissue engineering applications. It is expected that the analysis of alginate, gelatine and hydroxyapatite in terms of 3D bioprinting, clinical outcomes, scaffold architecture, and the regenerative medicine approach will enhance the research into bone tissue engineering in the near future. Continued studies into these research fields are highly recommended.

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Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect

Cited by 52 publications

References 68 publications

Autologous adipose-derived mesenchymal stem cells and hydroxyapatite for bone defect in rabbits

Autologous adipose-derived mesenchymal stem cells and hydroxyapatite for bone defect in rabbits

Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

A Bibliometric Analysis of the Global Trend of Using Alginate, Gelatine, and Hydroxyapatite for Bone Tissue Regeneration Applications

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