Pollination-induced oxidative stress in floral organs of Cymbidium pendulum (Roxb.) Sw. and Cymbidium aloifolium (L.) Sw. (Orchidaceae): A biochemical investigation

Attri, Lucky K; Nayyar, Harsh; Bhanwra, R. K.; Pehwal, Anju

doi:10.1016/j.scienta.2008.01.009

Cited by 13 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, there is good agreement between different floral species that levels of ASC fall with petal senescence. This fall often starts from bud stage onwards (orchids: Attri et al . 2008a,b; chrysanthemum: Bartoli et al .…”

Section: Oxidation Levels: Ascorbate (Asc) and Glutathione (Gsh)mentioning

confidence: 99%

“…ASC levels do not only fall in petals with senescence. In orchid species, ASC fell in all four floral organs tested: perianth, lip, column and ovary (Attri et al . 2008a,b).…”

Section: Oxidation Levels: Ascorbate (Asc) and Glutathione (Gsh)mentioning

confidence: 99%

“…The burst of ethylene production is thought to be activated by interaction between the pollen and stigma, although the primary signal has not been unequivocally identified (Rogers 2006). In several orchid species tested, pollination induced an increase in electrolyte leakage, lipid peroxidation and H 2 O 2 content, and a reduction in ASC levels (Attri et al . 2008a,b).…”

Section: Ros and Redox Signalling And Interactions With Pgr Signallingmentioning

confidence: 99%

See 2 more Smart Citations

Is there an important role for reactive oxygen species and redox regulation during floral senescence?

Rogers

2011

Plant Cell & Environment

View full text Add to dashboard Cite

Senescence is a highly regulated process terminating with programmed cell death (PCD). Floral senescence, and in particular petal senescence, forms an interesting model to study this process in that floral lifespan is species specific and linked to biological function. A feature of petal senescence is a rise in reactive oxygen species (ROS) and a change in redox balance. A key question is whether this is merely a consequence of de-regulation of antioxidant systems as cells enter PCD, or whether the rise in ROS may have a regulatory or signalling function. An important division in the physiology of floral senescence is between species in which ethylene is a key regulator, and those in which it appears not to perform an important regulatory role. Another important question we can therefore ask is whether the redox and ROS changes have the same significance in species with different physiologies. Transcriptomic studies in ethylene-sensitive and -insensitive species allow us to further determine whether changes in the activity of ROS-scavenging enzymes are transcriptionally regulated during floral senescence. Finally, it is important to assess how a signalling role for ROS or redox status would fit with known plant growth regulator (PGR) control of floral senescence.

show abstract

Section: Oxidation Levels: Ascorbate (Asc) and Glutathione (Gsh)mentioning

confidence: 99%

“…ASC levels do not only fall in petals with senescence. In orchid species, ASC fell in all four floral organs tested: perianth, lip, column and ovary (Attri et al . 2008a,b).…”

Section: Oxidation Levels: Ascorbate (Asc) and Glutathione (Gsh)mentioning

confidence: 99%

Section: Ros and Redox Signalling And Interactions With Pgr Signallingmentioning

confidence: 99%

See 1 more Smart Citation

Is there an important role for reactive oxygen species and redox regulation during floral senescence?

Rogers

2011

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…Pollination stimulated oxidative stress in floral organs, as evidenced by electrolyte leakage and high free radical levels in these tissues. 33 The PAT inhibitor, TIBA, and silver nitrate, an ethylene inhibitor, partially prevented this damage, suggesting that auxin-ethylene signaling plays a role in controlling oxidative stress responses during floral senescence. 34 Following pollination, an increase in hydrolytic activity produced soluble sugars, amino acids, and phosphorus in senescing petals and sepals.…”

mentioning

confidence: 98%

Role of Auxin in Orchid Development

Novak

Luna

Gamage

2014

Plant Signaling & Behavior

View full text Add to dashboard Cite

“…The activity of ROS-scavenging enzymes tends to increase during senescence [ 10 ]. Simultaneously, there is a concomitant increase in the level of malondialdehyde (MDA), which acts as an indicator of lipid peroxidation [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomic and Biochemical Changes during Senescence of Phalaenopsis ‘Red Dragon’ Petals

Chen

Zeng

2018

IJMS

View full text Add to dashboard Cite

Phalaenopsis flowers are some of the most popular ornamental flowers in the world. For most ornamental plants, petal longevity determines postharvest quality and garden performance. Therefore, it is important to have insight into the senescence mechanism of Phalaenopsis. In the present study, a proteomic approach combined with ultrastructural observation and activity analysis of antioxidant enzymes was used to profile the molecular and biochemical changes during pollination-induced petal senescence in Phalaenopsis “Red Dragon”. Petals appeared to be visibly wilting at 24 h after pollination, accompanied by the mass degradation of macromolecules and organelles during senescence. In addition, 48 protein spots with significant differences in abundance were found by two-dimensional electrophoresis (2-DE) and subjected to matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS). There were 42 protein spots successfully identified and homologous to known functional protein species involved in key biological processes, including antioxidant pathways, stress response, protein metabolism, cell wall component metabolism, energy metabolism, cell structure, and signal transduction. The activity of all reactive oxygen species (ROS)-scavenging enzymes was increased, keeping the content of ROS at a low level at the early stage of senescence. These results suggest that two processes, a counteraction against increased levels of ROS and the degradation of cellular constituents for maintaining nutrient recycling, are activated during pollination-induced petal senescence in Phalaenopsis. The information provides a basis for understanding the mechanism regulating petal senescence and prolonging the florescence of Phalaenopsis.

show abstract

Pollination-induced oxidative stress in floral organs of Cymbidium pendulum (Roxb.) Sw. and Cymbidium aloifolium (L.) Sw. (Orchidaceae): A biochemical investigation

Cited by 13 publications

References 28 publications

Is there an important role for reactive oxygen species and redox regulation during floral senescence?

Is there an important role for reactive oxygen species and redox regulation during floral senescence?

Role of Auxin in Orchid Development

Proteomic and Biochemical Changes during Senescence of Phalaenopsis ‘Red Dragon’ Petals

Contact Info

Product

Resources

About