Pollen morphology of selected species from the family Solanaceae

Song, Yuanyuan; Gu, Lei; Liu, Jiaxi

doi:10.1080/01916122.2018.1458663

Cited by 10 publications

(11 citation statements)

References 42 publications

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“…PollenCounter was initially developed to assess pollen viability in grapevine and showed excellent capacity to accurately estimate pollen number and pollen viability [13] . Pollen size and shape, which is an important parameter of the macro, vary greatly among plant species [2 , 12] . Tello et al.…”

Section: Introductionmentioning

confidence: 99%

Optimizing pollencounter for high throughput phenotyping of pollen quality in tomatoes

et al. 2020

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The macro “PollenCounter” in ImageJ was initially developed to assess pollen viability in grapevine. We set out to see if PollenCounter could be used to assess pollen number and viability in tomatoes. We tested different optimization scenarios by adjusting the pollen size (100–900, 200–900 pixel 2 ) and circularity of pollen grains (0.4–1, 0.5–1, and 0.6–1) on 31 microscopic images of stained tomato pollen. Both total pollen number and proportion of viable pollen were positively and significantly correlated with the outputs from manual counting. The scenario with 100–900 pixel 2 pollen size and 0.4–1 circularity had the highest association for pollen number ( r = 0.99) and pollen viability ( r = 0.86). PollenCounter is 32-fold faster than manual counting. We added a command to the macro to automatically save the outputs containing the number of total and viable pollen, avoiding transcription errors inherent to manual counting. We successfully applied the optimized PollenCounter to discriminate tomato genotypes based on pollen number and pollen viability under heat stress. Our results show that PollenCounter, as an open-access macro, can be customized and improved to meet users’ needs. The use of PollenCounter can save time and money in pollen quality assessment. We outline the steps to optimize the macro for other samples or crop species. The optimized macro could allow efficient screening of a large germplasm collection for pollen thermo-tolerance and selection of best thermo-tolerant individuals in breeding programs.

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Section: Introductionmentioning

confidence: 99%

Optimizing pollencounter for high throughput phenotyping of pollen quality in tomatoes

et al. 2020

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“…Solanaceae is identified by tricolporate and usually striate ornamentations in pollen grains. Song et al (2018) stated that the general shapes of pollen grains in Solanaceae are mainly oblate spheroidal or prolate spheroidal, rarely being prolate, suboblate or subprolate. The shape of the pollen grains in Lycium varies from prolate to preprloate in Iranian elements, but there were reports of subprolate, prolate-spheroidal, oblate-spheroidal and suboblate grains too [4].…”

Section: Discussionmentioning

confidence: 99%

Pollen Morphology of Lycium (Solanaceae) Species in Iran

MOUSAVIMANESH¹,

Keshavarzi²,

Bokaee³

2021

Contrib.Bot.

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Lycium (Solanaceae) has more than 100 species in arid and semi-arid parts of the world. In Iran, this medicinal plant is represented by 6 species, on which no palynological study has been conducted. Pollen morphology of 7 taxa are considered for the first time to evaluate the species relationships based on palynological data. Observations were made by use of Scanning electron microscopy on untreated pollen grains. Multivariate statistical analyses were carried out for qualitative and quantitative pollen features in the species studied. Results show that all pollen grains were monad, isopolar, zonotricolporate in prolate and preprolate shape. Exine sculpture was of striate and regulate patterns. Variations in pollen grains are considered to overcome the identification problems in species separation in morphologically similar taxa in this genus in Iran. The importance of pollen features in delimitation in some genera of Solanaceae was previously proven and the present study elucidates that some features as exine sculpture and some quantitative characters are of diagnostic importance in Lycium species native to Iran.

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“…Martins da Costa Rodrigues, Fernandes Falcão, Stehmann, and Girardi Bauermann () studied pollen morphology in Athenaea Sendtn and Aureliana Sendtn (Withaniinae, Solanaceae), the analysis showed that the pollen grains of both genera are very similar, differing in size‐related characters and also reported monad pollen grains with varying morphology, small to medium‐sized, lalongate endoapertures and long to extremely long colpi. Song, Gu, and Liu () also reported pollen morphology of selected species from the family Solanaceae from China and results suggested that the exine ornamentation of pollen grains could be divided into 11 types, made up of three types (cerebroid, granulate‐perforate‐punctate, and rugulate‐perforate).…”

Section: Discussionmentioning

confidence: 99%

Scanning Electron Microscopy (SEM) and Light Microscopy (LM)‐based Palyno‐morphological views of Solanaceae in Western Himalaya

Kayani

Hussain

Ahmad

et al. 2018

Microscopy Res & Technique

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In this study, plants belonging to family Solanaceae growing in Western Himalaya region have been observed palynologically under Light Microscope and Scanning electron microscope. Present investigation comprises of 10 genera and 23Withania somnifera. Solanaceae is a eurypalynous family. Grains are usually Tricolporate and Tetracolporate, radially symmetrical, isopolar, prolate-spheroidal to oblate-spheroidal to oblatespheroidal to subprolate to per prolate or suboblate to oblate, size range: 8.55-72 μm, amb circular, semi-angular or subangular, aperture drop-type, labrum common-type, exine usually 2 μm thick, nexine 1-1.5 μm thick. Tectum usually psilate, sexine reticulate, granulate or striato-reticulate, with obscure pattern, sexine 1-2 μm thick, nexine 1-1.5 μm thick, and intine 0.5-1 μm thick.Most striking variation has been found in the shape class, aperture-type, and tectal surface. Based on these characters, taxonomic keys have been made for correct identification of members in Solanaceae. However, the grains of this family are usually tricolporate and have direct relationship with certain members of the family Scrophulariaceae.Palyno-morphological characters of family Solanaceae have been studied for the first time in Western Himalayan region of Pakistan. These palyno-morphological characters are significant for identification of the members of family Solanaceae. K E Y W O R D S palynology, Solanaceae, western Himalaya

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Pollen morphology of selected species from the family Solanaceae

Cited by 10 publications

References 42 publications

Optimizing pollencounter for high throughput phenotyping of pollen quality in tomatoes

Optimizing pollencounter for high throughput phenotyping of pollen quality in tomatoes

Pollen Morphology of Lycium (Solanaceae) Species in Iran

Scanning Electron Microscopy (SEM) and Light Microscopy (LM)‐based Palyno‐morphological views of Solanaceae in Western Himalaya

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