Pollen Cryopreservation Protocol for Gloriosa superba L.

Rajasekharan, P. E.; Rohini, M. R.; Harsha, R.; Anilkumar, Gopinathan

doi:10.1007/978-1-0716-2843-0_33

Cited by 1 publication

(1 citation statement)

References 9 publications

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“…However, the only cryopreservation application for this species is described by Rajasekharan et al [112] for pollen conservation. In their study, the pollen was collected in the flowering phase in the early morning to ensure a high viability rate for explants, the pollen grains were desiccated to a moisture content of around 15-30% t before being plunged into liquid nitrogen.…”

Section: Colchicaceae Familymentioning

confidence: 99%

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

Merzougui

Benelli̇

Boullani

et al. 2023

Plants

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Nowadays, plant genetic resources are often at risk of loss and destruction. Geophytes are herbaceous or perennial species that are annually renewed by bulbs, rhizomes, tuberous roots, or tubers. They are often subject to overexploitation, which, combined with other biotic and abiotic stresses, can make these plants more vulnerable to a decline in their diffusion. As a result, multiple endeavors have been undertaken to establish better conservation strategies. Plant cryopreservation at ultra-low temperatures in liquid nitrogen (−196 °C) has proven to be an effective, long-term, low-cost, and suitable conservation method for many plant species. Over the last two decades, major advances in cryobiology studies have enabled successful explants of multiple genera and types, including pollen, shoot tips, dormant buds, and zygotic and somatic embryos. This review provides an update on recent advances and developments in cryopreservation and its application to medicinal and ornamental geophytes. In addition, the review includes a brief summary of factors limiting the success of bulbous germplasm conservation. The critical analysis underpinning this review will benefit biologists and cryobiologists in their further studies on the optimization of geophyte cryopreservation protocols and will support a more complete and wider application of knowledge in this area.

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Section: Colchicaceae Familymentioning

confidence: 99%