Polioviruses with Natural Recombinant Genomes Isolated from Vaccine-Associated Paralytic Poliomyelitis

Furione, Milena; Guillot, Sophie; Oțelea, Dan; Balanant, Jean; Candréa, A; Crainic, R

doi:10.1006/viro.1993.1468

Cited by 142 publications

(127 citation statements)

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“…Viruses were identified and typed by using a standard neutralizing assay with type-specific neutralizing antisera, as previously described (9,11). Intratypic differentiation between W and V strains was carried out with strain-specific monoclonal antibodies (5).…”

Section: Cells and Virusesmentioning

confidence: 99%

“…Recombinant genomes were detected with a double RFLP assay that has been described elsewhere (9). Briefly, two distant regions of the viral genome were subjected to PCR amplification followed by restriction enzyme digestion.…”

Section: Cells and Virusesmentioning

confidence: 99%

“…Restriction enzymes were selected to differentiate the three Sabin strains over the entire length of the genome. The pairs of primers and the restriction enzymes used in RFLP assays are listed in Table 1 (some of the primers presented have been used in previous studies [3,9,11,27]). …”

Section: Cells and Virusesmentioning

confidence: 99%

“…We detected a large number of vaccine/vaccine (V/V) recombinant strains (9). Most were easily recognized as intertypic V/V recombinants in restriction fragment length polymorphism (RFLP) tests, but we also observed many vaccine strains with genomic segments that gave atypical restriction patterns.…”

mentioning

confidence: 96%

“…Natural genetic recombination in PV, another mechanism of variation in Sabin strains, was demonstrated many years ago (14,32,33) (for reviews, see references 4 and 19). Sabin vaccine PV-derived strains with recombinant intertypic genomes have been found to occur naturally (17,22) and to be selected frequently in the gut of VAPP patients (9,11,21). In some cases, vaccine/ wild (V/W) PV recombinants have been found, in which vaccine-specific segments of the Sabin virus genome have been replaced by nonvaccine sequences derived from wild PVs or, perhaps, from non-polio enteroviruses (NPEVs) (10,19,21,30).…”

mentioning

confidence: 99%

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Natural Genetic Exchanges between Vaccine and Wild Poliovirus Strains in Humans

Guillot

Caro

Cuervo

et al. 2000

J Virol

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In a previous study of poliovirus vaccine-derived strains isolated from patients with vaccine-associated paralytic poliomyelitis (VAPP) (9, 11), we reported that a high proportion (over 50%) of viruses had a recombinant genome. Most were intertypic vaccine/vaccine recombinants. However, some had restriction fragment length polymorphism (RFLP) profiles different from those of poliovirus vaccine strains. We demonstrate here that five such recombinants, of 88 VAPP strains examined, carried sequences of wild (nonvaccine) origin. To identify the parental wild donor of these sequences, we used RFLP profiles and nucleotide sequencing to look for similarity in the 3D polymerase-coding region of 61 wild, cocirculating poliovirus isolates (43 type 1, 16 type 2, and 2 type 3 isolates). In only one case was the donor identified, and it was a wild type 1 poliovirus. For the other four vaccine/wild recombinants, the wild parent could not be identified. The possibility that the wild sequences were of a non-poliovirus-enterovirus origin could not be excluded. Another vaccine/wild recombinant, isolated in Belarus from a VAPP case, indicated that the poliovirus vaccine/wild recombination is not an isolated phenomenon. We also found wild polioviruses (2 of 15) carrying vaccine-derived sequences in the 3 moiety of their genome. All these results suggest that genetic exchanges with wild poliovirus and perhaps with nonpoliovirus enteroviruses, are also a natural means of evolution for poliovirus vaccine strains.

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Section: Cells and Virusesmentioning

confidence: 99%

Section: Cells and Virusesmentioning

confidence: 99%

Section: Cells and Virusesmentioning

confidence: 99%

mentioning

confidence: 96%

mentioning

confidence: 99%

See 3 more Smart Citations

Natural Genetic Exchanges between Vaccine and Wild Poliovirus Strains in Humans

Guillot

Caro

Cuervo

et al. 2000

J Virol

Self Cite

182

165

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Comparative study of poliovirus excretion after vaccination of infants with two oral polio vaccines prepared on vero cells or on primary monkey kidney cells

et al. 1997

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A comparative study was designed to assess the bioequivalence of 2 oral poliovaccines (OPV) produced on 2 different cell systems: primary monkey kidney (PMK) cells and the Vero cell line. The Vero cell line has been used to overcome the problem of obtaining a regular supply of high quality monkeys that are devoid of latent viruses. For this study, 9 children were vaccinated with PMK-OPV and 12 children with Vero-OPV. The comparison covered poliovirus excretion, reversion of polioviruses in the 5'-noncoding region, and immunogenicity. Major molecular markers in the 5'-noncoding region related to neurovirulence already had been identified at position 480 for type 1, position 481 for type 2, and position 472 for type 3 poliovirus. Two nucleic-acid based methods were designed for studying these positions: a RT-PCR followed by sequencing, which required preliminary culture and cloning; and a type-specific nested PCR followed by sequencing, which enabled direct detection and genotyping of polioviruses. Twenty-eight stool specimens were analyzed by this second method with no PCR inhibition problem. The use of Vero cell line did not modify the global pattern of poliovirus excretion, reversion frequency, or seroconversion. These results provide additional support for the use of the well-characterized Vero cell line in OPV manufacturing.

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Developed countries should use inactivated polio vaccine for the prevention of poliomyelitis

Plotkin

1997

Rev. Med. Virol.

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Polioviruses with Natural Recombinant Genomes Isolated from Vaccine-Associated Paralytic Poliomyelitis

Cited by 142 publications

References 0 publications

Natural Genetic Exchanges between Vaccine and Wild Poliovirus Strains in Humans

Natural Genetic Exchanges between Vaccine and Wild Poliovirus Strains in Humans

Comparative study of poliovirus excretion after vaccination of infants with two oral polio vaccines prepared on vero cells or on primary monkey kidney cells

Developed countries should use inactivated polio vaccine for the prevention of poliomyelitis

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