Poliovirus transcytosis through M-like cells

Ouzilou, Laurent; Caliot, Elise; Pelletier, Isabelle; Prévost, Marie‐Christine; Pringault, Eric; Colbère-Garapin, Florence

doi:10.1099/0022-1317-83-9-2177

Cited by 81 publications

(62 citation statements)

References 34 publications

(47 reference statements)

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“…32,33 In particular, some previous data suggest that poliovirus is not only neuro-tropic but also lymph node tropic. 31 Although many aspects of EV71 pathogenesis are currently unclear, some preliminary human autopsy reports and well-established murine and cynomolgus monkey models have demonstrated that, in some cases, EV71 pathogenesis occurs by causing neurological lesions in the CNS. 18,32,34 The observation of EV71-induced encephalomyelitis in human autopsies revealed that the CNS is a focal point for pathological lesions, although the data are limited.…”

Section: Discussionmentioning

confidence: 99%

“…31 Subsequently, the viruses proliferate in these primary organs and directly enter the bloodstream to establish systemic viremia, which is frequently linked to pathogenesis and the further infection of target tissues, such as the CNS and the myocardium. 32,33 In particular, some previous data suggest that poliovirus is not only neuro-tropic but also lymph node tropic.…”

Section: Discussionmentioning

confidence: 99%

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Pathogenesis study of enterovirus 71 infection in rhesus monkeys

Zhang

Cui

Liu

et al. 2011

Laboratory Investigation

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Enterovirus 71 (EV71), a major pathogen that is responsible for causing hand-foot-and-mouth disease (HFMD) worldwide, is a member of the Human Enterovirus species A, family Picornaviridae. HFMD that is caused by EV71 is usually characterized by vesicular lesions on the skin and oral mucosa and high morbidity rates in children; additionally, occasional fatal cases have been reported involving brainstem encephalitis and myelitis associated with cardiopulmonary collapse. Although viral pathogenesis in humans is unclear, previous animal studies have indicated that EV71, inoculated via various routes, is capable of targeting and injuring the central nervous system (CNS). We report here the pathogenic process of systemic EV71 infection in rhesus monkeys after inoculation via intracerebral, intravenous, respiratory and digestive routes. Infection with EV71 via these routes resulted in different rates of targeting to and injury of the CNS. Intracerebral inoculation resulted in pulmonary edema and hemorrhage, along with impairment of neurons. However, intravenous and respiratory inoculations resulted in a direct infection of the CNS, accompanied by obvious inflammation of lung tissue, as shown by impairment of the alveoli structure and massive cellular infiltration around the terminal bronchioles and small vessels. These pathological changes were associated with a peak of viremia and dynamic viral distribution in organs over time in the infected monkeys. Our results suggest that the rhesus monkey model may be used to study not only the basic pathogenesis of EV71 viral infections, but also to examine clinical features, such as neurological lesions, in the CNS and pathological changes in associated organs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pathogenesis study of enterovirus 71 infection in rhesus monkeys

Zhang

Cui

Liu

et al. 2011

Laboratory Investigation

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“…Indeed, we confirmed that the GII.4-Sydney HuNoV could cross a confluent intestinal epithelial monolayer to infect underlying B cells in the basal chamber of a transwell system. Many other types of viruses can be transcytosed across a polarized epithelium often in the absence of viral replication in the epithelial cells (e.g., [34][35][36][37][38][39] ) so this appears to be a common strategy for viruses to breach barriers along mucosal surfaces. Important areas of future research will be to determine whether the enterocytes transcytosing NoVs recognize pathogen-associated molecular patterns (PAMPs) and contribute to the antiviral immune response; and whether antiviral immune responses that prevent transcytosis are important in controlling NoV infection.…”

Section: A Gii4-sydney Human Norovirus Infects B Cells In Vitromentioning

confidence: 99%

Identification of a novel cellular target and a co-factor for norovirus infection – B cells & commensal bacteria

Karst

2015

Gut Microbes

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“…6,7 Transcytosis pathways are not only employed by blood components, but also adopted by viruses such as human immunodeficient viruses, adeno-associated virus and poliovirus. [8][9][10] Recent studies discovered that human melanotransferrin (MTf, also named P97), a transferrin homolog originally identified in human melanoma, was highly accumulated into the mouse brain following intravenous injection. 11,12 These studies demonstrated that MTf could cross the brain endothelial cells without affecting the integrity of the BBB and with a much higher rate than what is seen with transferrin and albumin.…”

Section: Introductionmentioning

confidence: 99%

Directing adenovirus across the blood–brain barrier via melanotransferrin (P97) transcytosis pathway in an in vitro model

et al. 2006

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Adenovirus serotype 5 (Ad5) is widely used in the development of gene therapy protocols. However, current gene therapy strategies involving brain are mostly based on intracranial injection. A major obstacle for systemically administered vectors to infect brain tissue is the blood-brain barrier (BBB). One strategy to cross the BBB is transcytosis, a transcellular transport process that shuttles a molecule from one side of the cell to the other side. Recently, melanotransferrin (MTf)/P97 was found to be able to cross the BBB and accumulate in brain. We thus hypothesize that re-directing Ad5 vectors to the MTf transcytosis pathway may facilitate Ad5 vectors to cross the BBB. To test this hypothesis, we constructed a bi-specific adaptor protein containing the extracellular domain of the coxsackie-adenovirus receptor (CAR) and the full-length melanotransferrin (sCAR-MTf), and investigated its ability to re-direct Ad5 vectors to the MTf transcytosis pathway. We found this adaptor protein could redirect Ad5 to the MTf transcytosis pathway in an in vitro BBB model, and the transcytosed Ad5 viral particles retained their native infectivity. The sCAR-MTf-mediated Ad5 transcytosis was temperature-and dose dependent. In addition, we examined the directionality of sCAR-MTf-mediated Ad5 transcytosis, and found the efficiency of apical-to-basal transcytosis was much higher than that of basal-to-apical direction, supporting a role of this strategy in transporting Ad5 vectors towards the brain. Taken together, our study demonstrated that re-directing Ad5 to the MTf transcytosis pathway could facilitate gene delivery across the BBB.

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Poliovirus transcytosis through M-like cells

Cited by 81 publications

References 34 publications

Pathogenesis study of enterovirus 71 infection in rhesus monkeys

Pathogenesis study of enterovirus 71 infection in rhesus monkeys

Identification of a novel cellular target and a co-factor for norovirus infection – B cells & commensal bacteria

Directing adenovirus across the blood–brain barrier via melanotransferrin (P97) transcytosis pathway in an in vitro model

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