Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)

Abstract

ABSTRACT. Enzymatic polymorphism in Melipolla quadriJasciata alltllidioidesLepeletier populations (Hymenoptera, Apidae, Meliponinae). Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations ti'om Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel eleclrophoresis ín order to investigate the genelic varíalion of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), a·glycerophos· phate dehydrogenase (aPGD), malate dehydrogenase (MDH), leucine aminopeplidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles (est·sand and est-f) while ali the others were shown to be monomorphic. The allele EST-S showed a frequency 01'82,6%.