Polarographic Assay of Hydrogen Peroxide Accumulation in Microbial Cultures

Dempsey, Patrick M.; O’Leary, Joseph S.; Condón, S.

doi:10.1128/aem.29.2.170-174.1975

Cited by 10 publications

(4 citation statements)

References 5 publications

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“…Hydrogen peroxide assay. The method used was a modification of that of Dempsey et al (5). A Beckman oxygen analyzer (model 1008) was used to measure the increase in dissolved 02 content of media containing H202 on addition of catalase.…”

Section: Methodsmentioning

confidence: 99%

“…Aeration of anaerobically grown cultures of the photosynthetic R. spheroides (2) and S. cerevisiae (20) also caused an increase in catalase activity. This might be explained by assuming that these cells have oxidase and/or superoxide dismutase enzymes and that H202 accumulates as a consequence of aeration, just as occurs in the essentially fermentative streptococci (1,5). 20 Other observations of increases of catalase synthesis do not, however, readily fit a hypothesis of H202 induction.…”

Section: ___mentioning

confidence: 99%

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Regulation of catalase synthesis in Salmonella typhimurium

Finn¹,

Condón²

1975

J Bacteriol

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The specific activity of catalase in Salmonella typhimurium and other enteric bacteria decreased during the logarithmic phase of growth and increased at the onset and during the stationary phase. The increase in catalase synthesis at the end of the exponential phase in S. typhimurium cells coincided with the lowest pH value reached by the culture. Maintenance of the pH at a constant neutral value did not alter the typical pattern of synthesis in contradiction of the results previously reported (McCarthy and Hinshelwood. 1959). A sudden decrease in the pH value of an S. typhimurium culture during exponential growth by addition of' HCI did not cause an alteration in the catalase synthesis pattern. Addition of hydrogen peroxide to S. typhimurium cultures within the range 1 AM to 2 mM during the exponential growth phase stimulated catalase synthesis. The extent of catalase synthesis depended on the concentration of hvdrogen peroxide; the maximum stimulation was observed at 80 AM. Increased catalase synthesis was not detected for 10 to 15 min after hydrogen peroxide addition. Hydrogen peroxide was produced by S. typhimurium cultures during the exponential and stationary growth phases. However, no direct relationship between hydrogen peroxide accumulation and synthesis of catalase was observed.

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Section: Methodsmentioning

confidence: 99%

Section: ___mentioning

confidence: 99%

Regulation of catalase synthesis in Salmonella typhimurium

Finn¹,

Condón²

1975

J Bacteriol

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“…L(+) lactate, pyruvate and formate were assayed enzymically according to Gutmann & Wahlefeld (1974), Czok & Lamprecht (1974), and Quayle (1966) respectively. Hydrogen peroxide was assayed with an oxygen electrode (Dempsey et al 1975). Each analysis was made in duplicate and if the duplicates varied by more than 10% the analysis was repeated.…”

Section: H E M I C a L A N A L Y S I Smentioning

confidence: 99%

Impact of aeration on the metabolic end‐products formed from glucose and galactose by Streptococcus lactis

Cogan¹,

Walsh²,

Condón³

1989

Journal of Applied Bacteriology

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S. 1989. Impact of aeration on the metabolic end-products formed from glucose and galactose by Streptococcus lactis. 66,77-84. Compared with cultures grown aerobically in batch culture with glucose, aerated cultures of lactic streptococci had a less homolactic type of metabolism when galactose was the carbohydrate source in batch cultures, or when glucose was limiting in chemostat cultures. Differences in end-products of sugar metabolism between aerated and unaerated cultures were observed. In addition to lactate, formate, acetate and ethanol were produced in anaerobic cultures, whereas acetate and acetoin were formed in aerated cultures. Acetate production in aerated cultures depended on lipoic acid, an essential cofactor of the pyruvate dehydrogenase complex. In a chemically defined medium with glucose as the energy substrate, lipoic acid (or acetate) was an essential growth factor. Formation of acetoin was inversely related to lipoic acid concentration in the growth medium. Although not observed in unaerated cultures, acetoin (and 2,3-butanediol) was produced in unaerated buffered suspensions metabolizing pyruvate. Aeration caused a modest increase in the activities of a-acetolactate synthetase and phosphate acetyl transferase, but it is unlikely that the increases were sufficient to account for the changes in end-products of sugar metabolism observed. Journal of Applied Bacteriology

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“…The Clarke electrode was connected to an oxygen monitor and pH meter (type PHA 927/PHM 27; Radiometer, Copenhagen, Denmark). Hydrogen peroxide was determined according to Dempsey et al (10).…”

Section: Methodsmentioning

confidence: 99%

Bactericidal Effect of Anaerobic Broth Exposed to Atmospheric Oxygen Tested on Peptostreptococcus anaerobius

Frölander

Carlsson

1977

J Clin Microbiol

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Peptostreptococcus anaerobius strain VPI 4330-1 was used as the test organism in an evaluation of the bactericidal effect of anaerobic broth exposed to air. The test organism, grown under anaerobic conditions in Trypticase soy broth, was diluted in buffered salt solution, and about 2 × 10 4 cells were suspended in 10 ml of an aerated broth. Ninety percent of the cells were killed within 15 min in actinomyces broth and within 50 min in Trypticase soy broth. All cells survived for 2 h in fluid thioglycolate medium. Addition of DABCO [1,4-diazabicyclo (2.2.2) octane] or mannitol to Trypticase soy broth did not influence the death rate of the organism, whereas superoxide dismutase decreased the death rate. Addition of catalase or manganese dioxide to the broth kept all the cells viable for 2 h. Of the three broth media tested, actinomyces broth reduced oxygen at the highest rate and Trypticase soy broth reduced it at the slowest rate. Hydrogen peroxide could be demonstrated in actinomyces broth and in Trypticase soy broth but not in fluid thioglycolate medium. In addition to catalase, manganese dioxide also removed all hydrogen peroxide from Trypticase soy broth, and superoxide dismutase significantly decreased the concentration of hydrogen peroxide in the broth. The results suggest that hydrogen peroxide mediated the toxic effect of atmospheric oxygen in these broth media.

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Polarographic Assay of Hydrogen Peroxide Accumulation in Microbial Cultures

Cited by 10 publications

References 5 publications

Regulation of catalase synthesis in Salmonella typhimurium

Regulation of catalase synthesis in Salmonella typhimurium

Impact of aeration on the metabolic end‐products formed from glucose and galactose by Streptococcus lactis

Bactericidal Effect of Anaerobic Broth Exposed to Atmospheric Oxygen Tested on Peptostreptococcus anaerobius

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