Regulation of catalase synthesis in Salmonella typhimurium

Abstract

The specific activity of catalase in Salmonella typhimurium and other enteric bacteria decreased during the logarithmic phase of growth and increased at the onset and during the stationary phase. The increase in catalase synthesis at the end of the exponential phase in S. typhimurium cells coincided with the lowest pH value reached by the culture. Maintenance of the pH at a constant neutral value did not alter the typical pattern of synthesis in contradiction of the results previously reported (McCarthy and Hinshelwood. 1959). A sudden decrease in the pH value of an S. typhimurium culture during exponential growth by addition of' HCI did not cause an alteration in the catalase synthesis pattern. Addition of hydrogen peroxide to S. typhimurium cultures within the range 1 AM to 2 mM during the exponential growth phase stimulated catalase synthesis. The extent of catalase synthesis depended on the concentration of hvdrogen peroxide; the maximum stimulation was observed at 80 AM. Increased catalase synthesis was not detected for 10 to 15 min after hydrogen peroxide addition. Hydrogen peroxide was produced by S. typhimurium cultures during the exponential and stationary growth phases. However, no direct relationship between hydrogen peroxide accumulation and synthesis of catalase was observed.

Differential Regulation of Two Divergent Sinorhizobium meliloti Genes for HPII-Like Catalases during Free-Living Growth and Protective Role of Both Catalases during Symbiosis

“…These results are consistent with the hypothesis of a weak KatC contribution to total catalase activity during exponential and early stationary phases and are consistent with the observation that the increase in total catalase activity during late stationary phase was mainly due to a rise in KatC activity. Very similar kinetics of catalase induction have been observed in cultures of other organisms such as E. coli (17), Salmonella typhimurium (11), Haemophilus parainfluenzae (35), and Rhodobacter sphaeroides (2), indicating a conserved strategy for surviving in starvation conditions. High similarity was observed between KatC and HPII regulations in that both catalases are upregulated by stationary phase but not by H 2 O 2 (25).…”

Regulation of galactose oxidase synthesis and secretion in Dactylium dendroides: effects of pH and culture density

“…The appearance of extracellular GOase accompanies the growth of D. dendroides at all stages, with the major portion being synthesized and secreted during the logarithmic phase of growth. The appearance of an extracellular enzyme in large quantity throughout the growth cycle is somewhat unusual, since many enzymes of this class, such as the bullulanases of Klebsiella aerogenes (3), catalase of Salmonella typhimurium (7), superoxide dismutase of Escherichia coli (13), and several proteases from Neurospora crassa (4), appear almost entirely during the stationary phase of the life cycle. Thus, GOase secretion by D. dendroides appears to be a normal cell process rather than a result of cell lysis.…”

Peroxide sensitivity of cold‐shocked Salmonella typhimurium and Escherichia coli and its relationship to minimal medium recovery

“…typhimurium were lower in cells grown in M9 than in those grown in TSYB, which was consistent with their relative peroxide sensitivity following cold shock (as indicated by the magnitude of the MMR effect). The absolute activities were low because cells were harvested during early exponential growth when catalase and peroxidase activities are at a minimum (Finn & Condon 1975;Hassan & Fridovich 1978).…”

“…Exposure of E . roli and salmonellas to low levels of hydrogen peroxide induces synthesis of catalase and other defences against oxidative stress (Finn & Condon 1975;Richter & Loewen 1981;. It was of interest, therefore, to see whether prior treatment with peroxide would induce resistance to the MMR effect in cells growing in minimal medium.…”