Polarized Photometric Detector for High-Performance Liquid Chromatography

Yamamoto, Atsushi; Matsunaga, Akira; Hayakawa, Kazuichi; Mizukami, Eiichi; Miyazaki, Motoichi

doi:10.2116/analsci.7.719

Cited by 16 publications

(6 citation statements)

References 5 publications

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“…In addition, any absorption of the sample at the selected wavelength will induce a reading offset, which is often much larger than the effect to be measured, and consequently difficult to compensate. 7 Alternative approaches based on the use of two polarizers and changing their relative orientation in the sample compartment of a single beam spectrophotometers were reported many years ago. These limiting factors may be due to some instability of the light source, which is not compensated in a single beam experiment.…”

Section: Intensity Methodsmentioning

confidence: 99%

“…A similar setup has been reported recently 6 with a diode array spectrophotometer, while the same approach had been devised in the past for high performance liquid chromatography (HPLC) OR detectors. 7 Alternative approaches based on the use of two polarizers and changing their relative orientation in the sample compartment of a single beam spectrophotometers were reported many years ago. 8,9 In the apparatus by Keston et al, which Very similar values are obtained by use of eq.…”

Section: Intensity Methodsmentioning

confidence: 99%

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Experimental methods for measuring optical rotatory dispersion: Survey and outlook

2011

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The measurement of optical rotation (OR) and optical rotatory dispersion has been finding renewed interest for some years, because of advancement in computational methods and in the performance of new experiments. Here, we shortly review the traditional and most-used experimental methods. We define and discuss the two main types of approaches in measuring OR: the intensity method and the optical null method. We report on some new results obtained by redesigning experiments based on the first approach, by adapting nonsophisticated hardware to current circular dichroism instrumentation.

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Section: Intensity Methodsmentioning

confidence: 99%

Section: Intensity Methodsmentioning

confidence: 99%

Experimental methods for measuring optical rotatory dispersion: Survey and outlook

2011

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“…We first developed a non-modulated OR detector. 1,2 We then developed a more sensitive CD detector 3 and a fluorescence-detected CD (FDCD) spectrometer, 4 which used a modulated beam in the wavelength axial direction. As is obvious from comparisons between OR, CD, and FDCD, the sensitivities are in the order OR < CD < FDCD whereas the number of compounds that can be analyzed is in the reverse order.…”

Section: Introductionmentioning

confidence: 99%

An Optical Rotatory Detector for High-Performance Liquid Chromatography Using Polarization Modulation

Yamamoto

Kawai²,

Sakamoto³

et al. 2008

ANAL. SCI.

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A sensitive and variable-wavelength optical rotatory (OR) detector for high-performance liquid chromatography is presented. This design is entirely different from that of conventional OR detectors consisting of a crossed polarizer pair. By placing a polarizing prism and a retardation plate into a commercial circular dichroism (CD) detector, the OR signal was obtained. The Mueller matrix approach was used to prove the principle of the OR signal appearance. Sugars and 4-androstene-3,17-dione were chosen as test compounds. The limit of detection was below 0.5 μg of injected sucrose at 260 nm, which was superior to that obtained with a conventional OR detector. For 4-androstene-3,17-dione, which is CD active, and shows a large anomalous OR dispersion curve, our detector gave a large OR signal with approximately half the intensity of the CD signal at 340 nm.

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“…We have proposed a novel, non-modulated polarimetric detector, a polarized photometric detector (PPD) (Yamamoto et al, 1991), and have continued research on its fundamental theory and applications Yamamoto et al, 1996;Yamamoto et al, 1997). In these processes, we have developed a split-type flow cell for the purpose of removing the influences of absorbance and/or RI changes followed by the analyte elution (Yamamoto et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

HPLC/circular dichroism detection using a split-type flow cell for polarized photometric detector

Yamamoto

Matsunaga

Hayakawa

et al. 1997

Biomed. Chromatogr.

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HPLC/circular dichroism detection using a conventional spectrophotometric detector has been established. A split‐type flow cell developed for a polarized photometric detector, which is a non‐modulated polarimeter, was inserted into this detector. When two rays of circularly polarized light with opposite rotational senses to each other were incident in both cells by combining a polarizer and a 1/4 wave retarder in front of the cell assembly, the output from this spectrophotometric detector became solely the signal based on circular dichroism. Selective detection of a chiral analyte, in which the ratio of the difference in the absorbance between two rays mentioned above and the absorbance by normal rays is over 1/1000, is feasible using the proposed instrument. © 1997 John Wiley & Sons, Ltd.

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Polarized Photometric Detector for High-Performance Liquid Chromatography

Cited by 16 publications

References 5 publications

Experimental methods for measuring optical rotatory dispersion: Survey and outlook

Experimental methods for measuring optical rotatory dispersion: Survey and outlook

An Optical Rotatory Detector for High-Performance Liquid Chromatography Using Polarization Modulation

HPLC/circular dichroism detection using a split-type flow cell for polarized photometric detector

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