HPLC/circular dichroism detection using a split-type flow cell for polarized photometric detector

Abstract

HPLC/circular dichroism detection using a conventional spectrophotometric detector has been established. A split‐type flow cell developed for a polarized photometric detector, which is a non‐modulated polarimeter, was inserted into this detector. When two rays of circularly polarized light with opposite rotational senses to each other were incident in both cells by combining a polarizer and a 1/4 wave retarder in front of the cell assembly, the output from this spectrophotometric detector became solely the signal based on circular dichroism. Selective detection of a chiral analyte, in which the ratio of the difference in the absorbance between two rays mentioned above and the absorbance by normal rays is over 1/1000, is feasible using the proposed instrument. © 1997 John Wiley & Sons, Ltd.

Multi-beam circular dichroism detector for HPLC

“…Another instrument built in our laboratory is equipped with a split-type flow-cell. 6 In this type of instrument, two photodiodes are used to offset the noise of the light source so that CD can be measured directly with high sensitivity. In this system, however, individual differences among diodes cause an increase in the background absorption signal, which makes it difficult to maintain the baseline.…”