Polarization properties of single layers in the posterior eyes of mice and rats investigated using high resolution polarization sensitive optical coherence tomography

Abstract

Abstract:We present a high resolution polarization sensitive optical coherence tomography (PS-OCT) system for ocular imaging in rodents. The system operates at 840 nm and uses a broadband superluminescent diode providing an axial resolution of 5.1 µm in air. PS-OCT data was acquired at 83 kHz A-scan rate by two identical custom-made spectrometers for orthogonal polarization states. Pigmented (Brown Norway, Long Evans) and non-pigmented (Sprague Dawley) rats as well as pigmented mice (C57BL/6) were imaged. Melanin pigment related depolarization was analyzed in the retinal pigment epithelium (RPE) and choroid of these animals using the degree of polarization uniformity (DOPU). For all rat strains, significant differences between RPE and choroidal depolarization were observed. In contrast, DOPU characteristics of RPE and choroid were similar for C57BL/6 mice. Moreover, the depolarization within the same tissue type varied significantly between different rodent strains. Retinal nerve fiber layer thickness, phase retardation, and birefringence were mapped and quantitatively measured in Long Evans rats in vivo for the first time. In a circumpapillary annulus, retinal nerve fiber layer birefringence amounted to 0.16°/µm ± 0.02°/µm and 0.17°/µm ± 0.01°/µm for the left and right eyes, respectively. 934-936 (1997). ©2016 Optical Society of America

Estimation of Jones matrix, birefringence and entropy using Cloude-Pottier decomposition in polarization-sensitive optical coherence tomography

“…To visualize the polarization scrambling, DOPU was developed and applied to the identification of the RPE [21,[55][56][57][58][59]75,76]. In addition, the bias correction of DOPU was suggested [49].…”

“…6(g) showed moderately high entropy as indicated by the light-green arrows. This result is because of melanin in the pigmented tissues, which is common in the retinal pigment epithelium (RPE) [21,[54][55][56][57][58][59]. High entropy of the cornea indicated by red arrows in Fig.…”

Polarization sensitive optical coherence tomography – a review [Invited]

“…A more detailed analysis of the birefringence of this layer by PS-OCT can be found in ref [112]. Although the limited space does not allow to discuss these applications and results in more detail, it should be mentioned that several papers have reported interesting and useful results of PS-OCT imaging of birefringent structures in animal eyes (cf., e.g., [63,[113][114][115][116]). …”

“…Several other diseases that affect the macula have been successfully studied by PS-OCT like diabetic retinopathy [129], central serous chorioretinopathy [130], telangiectasia [131], or hereditary diseases like albinism [132] and Stargardt's disease [133]. Moreover, related studies in animal models have been performed [116,134,135]. A detailed description of these results is, however, beyond the scope of this paper.…”

In Vivo Characterization of Spontaneous Retinal Neovascularization in the Mouse Eye by Multifunctional Optical Coherence Tomography

“…In this work we utilized a multifunctional OCT imaging system that was described in detail by Fialová et al 43 and has been recently used to image mouse 41 and rat models 42,44 of ophthalmic diseases. In short, the system is based on a SD, PS-OCT unit enabling high-speed retinal imaging at 83-kHz Ascan rate with an axial resolution of 3.8 lm in retinal tissue.…”