Polarization and phase pulse shaping applied to structural contrast in nonlinear microscopy imaging

Schön, Peter; Behrndt, Martin; Aït-Belkacem, Dora; Rigneault, Hervé; Brasselet, Sophie

doi:10.1103/physreva.81.013809

Cited by 29 publications

(32 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In practice, strong focusing conditions involve an E Z component which can raise up to 40% in amplitude compared to in-plane components [22]. This contribution will be neglected here since, in the case of the type of distributions studied here, only a strong out-of-plane tilt of the sample can lead to a noticeable bias in the nonlinear polarized measurements [23,24]. Each remaining in-plane tensorial component of the previous expression can then be expressed more specifically, for instance,…”

Section: B Effective Distribution Functionmentioning

confidence: 99%

Structure of molecular packing probed by polarization-resolved nonlinear four-wave mixing and coherent anti-Stokes Raman-scattering microscopy

et al. 2014

Self Cite

View full text Add to dashboard Cite

International audienceWe report a method that is able to provide refined structural information on molecular packing in biomolecular assemblies using polarization-resolved four-wavemixing and coherent anti-Stokes Raman-scattering microscopy. These third-order nonlinear processes allow quantifying high orders of symmetry which are exploited here to reveal a high level of detail in the angular disorder behavior at the molecular scale in lipid membranes

show abstract

Section: B Effective Distribution Functionmentioning

confidence: 99%

Structure of molecular packing probed by polarization-resolved nonlinear four-wave mixing and coherent anti-Stokes Raman-scattering microscopy

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Amplitude shaping to select the fundamental excitation spectra is practically robust and simple, and phase shaping in addition can compensate the dispersion to higher order to enhance the excitation efficiency [16], [17]. To date, pulse shaping techniques have not found wide applications in SHG and THG imaging [18], [19]. This method can also be applied for SHG and THG imaging with arbitrary spectral range within the fundamental spectrum to improve efficiency.…”

Section: Introductionmentioning

confidence: 99%

Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

Liu

Benalcazar

et al. 2012

IEEE J. Select. Topics Quantum Electron.

View full text Add to dashboard Cite

Nonlinear microscopy has become widely used in biophotonic imaging. Pulse shaping provides control over nonlinear optical processes of ultrafast pulses for selective imaging and contrast enhancement. In this study, nonlinear microscopy, including two-photon fluorescence, second harmonic generation, and third harmonic generation, was performed using pulses shaped from a fiber supercontinuum (SC) spanning from 900 to 1160 nm. The SC generated by coupling pulses from a Yb:KYW pulsed laser into a photonic crystal fiber was spectrally filtered and compressed using a spatial light modulator. The shaped pulses were used for nonlinear optical imaging of cellular and tissue samples. Amplitude and phase shaping the fiber SC offers selective and efficient nonlinear optical imaging over a broad bandwidth with a single-beam and an easily tunable setup.

show abstract

“…In this work, we use a degenerate scheme, where two input fields have the same angular frequency ω 1 ω 2 and the third one is set to the angular frequency ω 3 . In the planar wave approximation, which is found to be valid in this study [19,35], the measured intensity is proportional to the modulus square of the induced nonlinear polarization P FWM , at angular frequency ω 0 2ω 1 − ω 3 , and can be written as…”

Section: B From Microscopic To Macroscopic Susceptibilitymentioning

confidence: 86%

Polarization-resolved four-wave mixing microscopy for structural imaging in thick tissues

2012

Self Cite

View full text Add to dashboard Cite

We present a polarimetric analysis of the four-wave mixing (FWM) signal emitted by thick tissues, in order to extract structural information on molecular order and orientation. A careful analysis of the polarization distortions introduced by the birefringence of the sample is conducted for the proper interpretation of the results. FWM, compared to other well-known nonlinear optical methods such as second-harmonic generation, gives access to additional information on the symmetry of the molecular distribution. Furthermore, it brings the advantage of being generated in any kind of sample, even when centrosymmetry is present. The model developed here is applied to thick rat-tail tendon samples, composed essentially of collagen fibers. We show that, once the birefringence of the sample is completely characterized, it is possible to retrieve the even-order components of the molecular orientational distribution up to the fourth order of symmetry.

show abstract

Polarization and phase pulse shaping applied to structural contrast in nonlinear microscopy imaging

Cited by 29 publications

References 55 publications

Structure of molecular packing probed by polarization-resolved nonlinear four-wave mixing and coherent anti-Stokes Raman-scattering microscopy

Structure of molecular packing probed by polarization-resolved nonlinear four-wave mixing and coherent anti-Stokes Raman-scattering microscopy

Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

Polarization-resolved four-wave mixing microscopy for structural imaging in thick tissues

Contact Info

Product

Resources

About