Point prevalence of infection with Mycoplasma bovoculi and Moraxella spp. in cattle at different stages of infectious bovine keratoconjunctivitis

Schnee, Christiane; Heller, Martin; Schubert, Evelyn; Sachse, Konrad

doi:10.1016/j.tvjl.2014.11.009

Cited by 32 publications

(16 citation statements)

References 24 publications

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“…M. canis is a canine mycoplasma, but it is also increasingly isolated from cattle pneumonia cases (5,18). interestingly, M. bovoculi, which is responsible mainly for keratoconjunctivitis (18,26), was found in nasal swabs in our study. M. canadense is not able to induce lung infection without other pathogens (25).…”

Section: Discussionsupporting

confidence: 53%

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Szacawa

Szymańska-Czerwińska

Niemczuk

et al. 2016

Journal of Veterinary Research

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Introduction: Mycoplasma bovis is one of the main pathogens involved in cattle pneumonia. Other mycoplasmas have also been directly implicated in respiratory diseases in cattle. The prevalence of different Mycoplasma spp. in cattle affected by respiratory diseases and molecular characteristics of M. bovis field strains were evaluated. Material and Methods: In total, 713 nasal swabs from 73 cattle herds were tested. The uvrC gene fragment was amplified by PCR and PCR products were sequenced. PCR/DGGE and RAPD were performed. Results: It was found that 39 (5.5%) samples were positive for M. bovis in the PCR and six field strains had point nucleotide mutations. Additionally, the phylogenetic analysis of 20 M. bovis field strains tested with RAPD showed two distinct groups of M. bovis strains sharing only 3.8% similarity. PCR/DGGE analysis demonstrated the presence of bacteria belonging to the Mollicutes class in 79.1% of DNA isolates. The isolates were identified as: Mycoplasma bovirhinis, M. dispar, M. bovis, M. canis, M. arginini, M. canadense, M. bovoculi, M. alkalescens, and Ureaplasma diversum. Conclusion: Different Mycoplasma spp. strains play a crucial role in inducing respiratory diseases in cattle.

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Section: Discussionsupporting

confidence: 53%

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Szacawa

Szymańska-Czerwińska

Niemczuk

et al. 2016

Journal of Veterinary Research

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“…Mycoplasma spp. is also highly prevalent in the conjunctivae of cattle with IBK, with Mycoplasma bovoculi being the most commonly identified species (Schnee et al, 2015;Vilei et al, 2007). Whether Mycoplasma bovoculi alone can cause clinical IBK, or act as a predisposing factor enhancing the effects of Mycoplasma spp.…”

Section: Discussionmentioning

confidence: 99%

“…Herds with a high prevalence of Mycoplasma bovoculi are more predisposed to acute IBK, possibly due to this pathogen facilitating enhanced infection with Moraxella spp. (Schnee et al, 2015). An outbreak of IBK has even been attributed to the synergistic action of Mycoplasma bovoculi and Mycoplasma bovis, following the possible predisposing effects of bovine respiratory disease (Levisohn et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Mycoplasma spp. have also been frequently reported as causal agents of bovine IBK, with Mycoplasma bovoculi being most prevalent (Li et al, 2015;Pinho et al, 2012;Schnee et al, 2015). One IBK outbreak was attributed to the synergistic action of Mycoplasma bovoculi and Mycoplasma bovis, following the possible predisposing effect of bovine respiratory disease (Levisohn et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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A multiplex real-time PCR assay for the detection and differentiation of five bovine pinkeye pathogens

Zheng

Porter

Noll

et al. 2019

Journal of Microbiological Methods

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A B S T R A C TInfectious bovine keratoconjunctivitis (IBK), also known as pinkeye, is one of the most common eye diseases in cattle. Several pathogens have been associated with IBK cases, however, Moraxella bovis, Moraxella bovoculi, Mycoplasma bovis, Mycoplasma bovoculi and bovine herpesvirus type 1 (BHV-1) are most frequently observed. A multiplex real-time PCR assay using two reactions was developed for the detection and differentiation of these five pathogens. Detection sensitivities of the multiplex assays were compared to singleplex reactions testing for the same targets. Correlation coefficients (R 2 ) of > 0.99, and PCR efficiencies between 92 and 106% were demonstrated in all singleplex and multiplex real-time PCR reactions. The limits of detection (LOD) of multiplex assays for Moraxella bovis, Moraxella bovoculi, Mycoplasma bovis, Mycoplasma bovoculi and BHV-1 were 19, 23, 25, 24 and 26 copies per reaction, respectively. No cross amplification was observed for specificity testing of 179 IBK positive clinical samples and 55 non-target clinical samples. Percentage of clinical samples positive for Mycoplasma bovoculi, Moraxella bovoculi, Moraxella bovis, BHV-1 and Mycoplasma bovis were 88.8% (159/179), 75.9% (136/179), 60.3% (108/179), 11.7% (21/179) and 10.0% (18/179), respectively. Moraxella bovis, Moraxella bovoculi and Mycoplasma bovoculi were more prevalent than Mycoplasma bovis and BHV-1 in IBK samples collected from animals in this study population. Our data indicates that the multiplex real-time PCR panel assay is highly sensitive and highly specific for the detection and differentiation of the five major pathogens associated with bovine pinkeye. Fig. 3. The prevalence of the five pathogens in IBK samples. Panel A: Prevalence of the five pathogens from the 179 bovine ocular swab samples that were subjected to multiplex real-time PCR testing. Panel B: Co-infections among the three most prevalent IBK pathogens in the 179 positive clinical samples: 95 (53.0%) samples were positive for both Moraxella bovis and Moraxella bovoculi; 100 (55.8%) samples were positive for both Moraxella bovis and Mycoplasma bovoculi; 129 (72.0%) samples were positive for both Moraxella bovoculi and Mycoplasma bovoculi and 93 (51.9%) samples were positive for Moraxella bovis, Moraxella bovoculi and Mycoplasma bovoculi. W. Zheng, et al.

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“…When compared with traditional culture, this method was not shown to be significantly more sensitive; however, it did allow organism speciation with several samples containing more than one species. SYBR green technology has also been used to develop a species specific PCR to detect M. bovoculi in conjunctival swabs from dairy calves and beef suckler cows as part of a point prevalence study into infectious bovine keratoconjunctivitis …”

Section: Molecular Diagnosismentioning

confidence: 99%

A review of mycoplasma diagnostics in cattle

Parker

Sheehy

Hazelton

et al. 2018

Veterinary Internal Medicne

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Mycoplasma species have a global distribution causing serious diseases in cattle worldwide including mastitis, arthritis, pneumonia, otitis media and reproductive disorders. Mycoplasma species are typically highly contagious, are capable of causing severe disease, and are difficult infections to resolve requiring rapid and accurate diagnosis to prevent and control disease outbreaks. This review discusses the development and use of different diagnostic methods to identify Mycoplasma species relevant to cattle, with a particular focus on Mycoplasma bovis. Traditionally, the identification and diagnosis of mycoplasma has been performed via microbial culture. More recently, the use of polymerase chain reaction to detect Mycoplasma species from various bovine samples has increased. Polymerase chain reaction has a higher efficiency, specificity, and sensitivity for laboratory diagnosis when compared with conventional culture‐based methods. Several tools are now available for typing Mycoplasma spp. isolates, allowing for genetic characterization in disease outbreak investigations. Serological diagnosis through the use of indirect ELISA allows the detection of antimycoplasma antibodies in sera and milk, with their use demonstrated on individual animal samples as well as BTM samples. While each testing method has strengths and limitations, their combined use provides complementary information, which when interpreted in conjunction with clinical signs and herd history, facilitates pathogen detection, and characterization of the disease status of cattle populations.

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Point prevalence of infection with Mycoplasma bovoculi and Moraxella spp. in cattle at different stages of infectious bovine keratoconjunctivitis

Cited by 32 publications

References 24 publications

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

A multiplex real-time PCR assay for the detection and differentiation of five bovine pinkeye pathogens

A review of mycoplasma diagnostics in cattle

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