Point‐of‐Care Platform for Rapid Multiplexed Detection of SARS‐CoV‐2 Variants and Respiratory Pathogens

Trick, Alexander Y.; Chen, Fan‐En; Chen, Liben; Lee, Pei-Wei; Hasnain, Alexander C; Mostafa, Heba H.; Carroll, Karen C.; Wang, Tza‐Huei

doi:10.1002/admt.202101013

Cited by 20 publications

(15 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although PCR amplification may require bulky and expensive lab equipment to perform the temperature fluctuation in the expansion process, as well as intense, trained human labor, Trick et al were able to overcome these limitations by integrating a magnetofluidic cartridge into an automated and portable PCR testing platform [ 120 ]. This tool utilized inexpensive components (e.g., 3D printing, laser cutting, and programed microcontroller) that combined the sample preparation steps and PCR to provide results in less than 30 min with minimal training required.…”

Section: Point-of-care Amenable Platformsmentioning

confidence: 99%

“…This tool utilized inexpensive components (e.g., 3D printing, laser cutting, and programed microcontroller) that combined the sample preparation steps and PCR to provide results in less than 30 min with minimal training required. The multiplexing is achieved by two fluorescence probes to identify B.1.1.7/B.1.351 variants and SARS-CoV-2/Influenza with a LOD of 12.5 copies/µL [ 120 ].…”

Section: Point-of-care Amenable Platformsmentioning

confidence: 99%

“…First, the various current reaction techniques reviewed herein have their unique remaining challenges. Despite reports of rapid and miniaturized RT-PCR [ 18 , 120 ], RT-PCR often still involves lengthy assay time and relies on a bulky thermocycler. RT-LAMP can still be susceptible to false amplification [ 56 , 124 ].…”

Section: Remaining Challenges and Future Perspectivesmentioning

confidence: 99%

See 2 more Smart Citations

Emerging Multiplex Nucleic Acid Diagnostic Tests for Combating COVID-19

et al. 2022

Self Cite

View full text Add to dashboard Cite

The COVID-19 pandemic caused by SARS-CoV-2 has drawn attention to the need for fast and accurate diagnostic testing. Concerns from emerging SARS-CoV-2 variants and other circulating respiratory viral pathogens further underscore the importance of expanding diagnostic testing to multiplex detection, as single-plex diagnostic testing may fail to detect emerging variants and other viruses, while sequencing can be too slow and too expensive as a diagnostic tool. As a result, there have been significant advances in multiplex nucleic-acid-based virus diagnostic testing, creating a need for a timely review. This review first introduces frequent nucleic acid targets for multiplex virus diagnostic tests, then proceeds to a comprehensive and up-to-date overview of multiplex assays that incorporate various detection reactions and readout modalities. The performances, advantages, and disadvantages of these assays are discussed, followed by highlights of platforms that are amenable for point-of-care use. Finally, this review points out the remaining technical challenges and shares perspectives on future research and development. By examining the state of the art and synthesizing existing development in multiplex nucleic acid diagnostic tests, this review can provide a useful resource for facilitating future research and ultimately combating COVID-19.

show abstract

Section: Point-of-care Amenable Platformsmentioning

confidence: 99%

Section: Point-of-care Amenable Platformsmentioning

confidence: 99%

Section: Remaining Challenges and Future Perspectivesmentioning

confidence: 99%

See 1 more Smart Citation

Emerging Multiplex Nucleic Acid Diagnostic Tests for Combating COVID-19

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…To date, researchers reported several POC devices for detection of SARS-CoV-2 ( Soares et al, 2021 ; Torezin Mendonça et al, 2022 ; Trick et al, 2022 ). These POC devices demonstrated a simple design and fast detection.…”

Section: Introductionmentioning

confidence: 99%

Point-of-care system for rapid real-time detection of SARS-CoV-2 virus based on commercially available Arduino platforms

Ngoc

Quyen

Vinayaka

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The COVID-19 pandemic emphasized the importance of rapid, portable, and on-site testing technologies necessary for resource-limited settings for effective testing and screening to reduce spreading of the infection. Realizing this, we developed a fluorescence-based point-of-care (fPOC) detection system with real-time reverse transcriptase loop-mediated isothermal amplification for rapid and quantitative detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. The system is built based on the Arduino platform compatible with commercially available open-source hardware–software and off-the-shelf electronic components. The fPOC system comprises of three main components: 1) an instrument with integrated heaters, 2) optical detection components, and 3) an injection-molded polymeric cartridge. The system was tested and experimentally proved to be able to use for fast detection of the SARS-CoV-2 virus in real-time in less than 30 min. Preliminary results of testing the performance of the fPOC revealed that the fPOC could detect the SARS-CoV-2 virus at a limit of detection (LOD50%) at two to three copies/microliter (15.36 copies/reaction), which was comparable to reactions run on a standard commercial thermocycler. The performance of the fPOC was evaluated with 12 SARS-CoV-2 clinical throat swab samples that included seven positive and five negative samples, as confirmed by reverse transcription–polymerase chain reaction. The fPOC showed 100% agreement with the commercial thermocycler. This simple design of the fPOC system demonstrates the potential to greatly enhance the practical applicability to develop a totally integrated point-of-care system for rapid on-site screening of the SARS-CoV-2 virus in the management of the pandemic.

show abstract

“…Owing to their high sensitivity and turnaround of 1-2 hours, NAATs are widely applied in medical diagnostics such as for HIV, tuberculosis, and SARS-CoV-2. [1][2][3] Polymerase chain reaction (PCR) and reverse transcription PCR are two of the most widely used techniques for detecting target DNAs or RNAs. Both techniques require a thermocycling process, which involves controlling the temperature of the reaction vessel to three distinct temperatures.…”

Section: Introductionmentioning

confidence: 99%