Point‐of‐care oral‐based diagnostics

Abstract

Many of the target molecules that reside in blood are also present in oral fluids, albeit at lower concentrations. Oral fluids are, however, relatively easy and safe to collect without the need for specialized equipment and training. Thus, oral fluids provide convenient samples for medical diagnostics. Recent advances in lab-on-a-chip technologies have made minute, fully integrated diagnostic systems practical for an assortment of point-of-care tests. Such systems can perform either immunoassays or molecular diagnostics outside centralized laboratories within time periods ranging from minutes to an hour. The article briefly reviews recent advances in devices for point-of-care testing with a focus on work that has been carried out by the authors as part of a NIH program.

Fundamentals and Applications of Immunosensors

“…The use of nanoparticles reduces the incubation time to a few minutes, while an analog ELISA usually would require 30-60 minutes incubation. Finally, multiple analytes detection (Chen et al, 2009), microfluidics (Bange et al, 2005) and lab-on-a-chip (Hart et al, 2011) concepts are clearly in the future of immunosensors, and many devices have been presented that may also find soon wide application, with great impact in health care assessment, especially in developing countries. Fig.…”

Salivary Diagnostics in Oral Cancer

“…Another assay that is employed at point of care is based on microbeads. Microbeads with various functionalization are ubiquitous solid supports for capturing target molecules in both bench top and microfluidic system (Hart et al, 2011).…”

A Review on Lateral Flow Test Strip for Food Safety

“…However, these methods require several steps, expensive instruments, and skilled operators (Hart et al, 2011;Hossain et al, 2012).…”