Point‐of‐Care Identification of Bacteria Using Protein‐Encapsulated Gold Nanoclusters

Ji, Haiwei; Li, Wu; Pu, Fang; Ren, Jinsong; Qu, Xiaogang

doi:10.1002/adhm.201701370

Cited by 61 publications

(37 citation statements)

References 32 publications

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“…To date, the chemical nose strategy has been applied to detect pathogenic bacteria in the context of diagnosing infectious diseases 36 , where libraries of fluorescent probe molecules 26,27,37 , polymers 38 , nanoparticles 39,40 and supramolecular complexes 41,42 have been developed to identify the pathogens. However, no chemical nose approach for gut-derived bacteria has been developed and reported yet.…”

Section: Discussionmentioning

confidence: 99%

Polymer-Based Chemical Nose Systems for Optical Pattern Recognition of Gut Microbiota

Tomita

Kusada²,

Kojima³

et al. 2020

Preprint

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Understanding the status of gut microbiota has been recognized as crucial in health management and disease treatment. To meet the demands of medical and biological applications where rapid evaluation of gut microbiota in limited research environment is essential, we developed new sensing systems able to readout the overall characteristics of complex microbiota. Response patterns generated by a synthetic library of 12 charged block-copolymers with aggregation-induced emission units were analyzed with pattern recognition algorithms, allowing to identify the species/phyla of 16 axenic cultures of intestinal bacterial strains. More importantly, our method clearly classified artificial models of obesity-associated gut microbiota, and further succeeded in detecting sleep disorders in mice through comparative analysis of the normal/abnormal mouse gut microbiota. Our techniques can analyze complex bacterial samples far more quickly, simply and inexpensively than common metagenome-based methods, offering a powerful and complementary tool for gut microbiome analysis for practical use, e.g., in clinical settings.

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Section: Discussionmentioning

confidence: 99%

Polymer-Based Chemical Nose Systems for Optical Pattern Recognition of Gut Microbiota

Tomita

Kusada²,

Kojima³

et al. 2020

Preprint

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“…Over the past years, a variety of metal NCs, including AuNCs [165] , [166] , AgNCs [167] , [168] , and so on, have been synthesized and employed for the POC detection of infectious diseases. For example, Ji et al [169] synthesized a protein-AuNC-based fluorescence sensor array for rapid identification of bacteria, which was able to realize discrimination of six kinds of bacteria, including two kinds of drug-resistant bacteria. Moreover, the sensor array could achieve 100% classification accuracy by using two protein-AuNCs probes with outstanding advantages of easy synthesis and convenient to use, demonstrating its promise for facile diagnosis of bacterial infection in resource-poor regions [169] .…”

Section: Detection Methods For Poc Diagnostic Applicationsmentioning

confidence: 99%

Point-of-care diagnostics for infectious diseases: From methods to devices

et al. 2021

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“…To achieve simultaneous detection of multiple bacteria, sensor arrays based on Au NCs have also been developed. For instance, Qu and coworkers designed and prepared a bacterial sensor array based on the integration of HSA-Au NCs, lysozyme (Lyz)-Au NCs, lactoferrin (Lf)-Au NCs, and vancomycin decorated HSA-Au NCs (Van-Au NCs) ( Figure 5) (Ji et al, 2018). HSA-Au NCs are selected based on the interaction between the peptide motifs on the surface of HSA and the bacterial cell wall (Chan and Chen, 2012).…”

Section: Sensor Arraysmentioning

confidence: 99%

Gold Nanoclusters for Bacterial Detection and Infection Therapy

et al. 2020

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Infections caused by antibiotic-resistant bacteria have become one of the most serious global public health crises. Early detection and effective treatment can effectively prevent deterioration and further spreading of the bacterial infections. Therefore, there is an urgent need for time-saving diagnosis as well as therapeutically potent therapy approaches. Development of nanomedicine has provided more choices for detection and therapy of bacterial infections. Ultrasmall gold nanoclusters (Au NCs) are emerging as potential antibacterial agents and have drawn intense attention in the biomedical fields owing to their excellent biocompatibility and unusual physicochemical properties. Recent significant efforts have shown that these versatile Au NCs also have great application potential in the selective detection of bacteria and infection treatment. In this review, we will provide an overview of research progress on the development of versatile Au NCs for bacterial detection and infection treatment, and the mechanisms of action of designed diagnostic and therapeutic agents will be highlighted. Based on these cases, we have briefly discussed the current issues and perspective of Au NCs for bacterial detection and infection treatment applications.

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Point‐of‐Care Identification of Bacteria Using Protein‐Encapsulated Gold Nanoclusters

Cited by 61 publications

References 32 publications

Polymer-Based Chemical Nose Systems for Optical Pattern Recognition of Gut Microbiota

Polymer-Based Chemical Nose Systems for Optical Pattern Recognition of Gut Microbiota

Point-of-care diagnostics for infectious diseases: From methods to devices

Gold Nanoclusters for Bacterial Detection and Infection Therapy

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