Point-of-care cancer diagnostic devices: From academic research to clinical translation

Syedmoradi, Leila; Norton, Michael L.; Omidfar, Kobra

doi:10.1016/j.talanta.2020.122002

Cited by 69 publications

(40 citation statements)

References 167 publications

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“…The large majority of current medical diagnostic strategies target protein biomarkers [5–7] and are based on the enzyme‐linked immunosorbent assay (ELISA) [8] . However, despite its high sensitivity, this assay possesses significant drawbacks that limit its use in point‐of‐care settings, such as high production costs, complex procedures, and the need for trained operators [9] .…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4] In this context, the use of biosensors to detect minute amount of cancer biomarkers in biological fluids appears as a promising strategy for the early diagnosis of cancer. [5] The large majority of current medical diagnostic strategies target protein biomarkers [5][6][7] and are based on the enzymelinked immunosorbent assay (ELISA). [8] However, despite its high sensitivity, this assay possesses significant drawbacks that limit its use in point-of-care settings, such as high production costs, complex procedures, and the need for trained operators.…”

Section: Introductionmentioning

confidence: 99%

“…Today, most of the cancer diagnosis are obtained via clinical imaging techniques that are not adapted to early diagnosis [1–4] . In this context, the use of biosensors to detect minute amount of cancer biomarkers in biological fluids appears as a promising strategy for the early diagnosis of cancer [5] …”

Section: Introductionmentioning

confidence: 99%

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Peptide‐Conjugated Silver Nanoparticles for the Colorimetric Detection of the Oncoprotein Mdm2 in Human Serum

et al. 2022

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The development of efficient, reliable, and easy-to-use biosensors allowing early cancer diagnosis is of paramount importance for patients. Herein, we report a biosensor based on silver nanoparticles functionalized by peptide aptamers for the detection of a cancer biomarker, i. e. the Mdm2 protein. Silver nanoparticles (AgNPs) were produced and stabilized with a thin PEGylated-calix[4]arene layer that allows (i) the steric stabilization of the AgNPs and (ii) the covalent conjugation of the peptide aptamers via the formation of an amide bond. These peptide-conjugated AgNPs were then used to detect Mdm2 via a dual trapping strategy that was previously reported with gold nanoparticles (AuNPs). Our results showed that replacing AuNPs by AgNPs improves the detection limit by nearly one order of magnitude, down to 5 nM, while the high selectivity of the system and the stability of the particles provided by the calixarene coating allow the detection of Mdm2 in human serum.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Peptide‐Conjugated Silver Nanoparticles for the Colorimetric Detection of the Oncoprotein Mdm2 in Human Serum

et al. 2022

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“…As a research tool, microfluidic plays a vital role in biochemical analysis (H. Lee et al, 2014; Li et al, 2014; Temmerman et al, 2004), which can be used for subsequent clinical diagnosis, nucleic acid detection, and protein analysis. Various microfluidic devices (Nguyen et al, 2019; Song et al, 2021; Syedmoradi et al, 2021) have been developed for other point‐of‐care testing (POCT). Seung et al proposed a centrifugal integrated microfluidic chip for detecting a variety of foodborne bacteria by color LAMP (Oh & Seo, 2019).…”

Section: Introductionmentioning

confidence: 99%

Stretch‐driven microfluidic chip for nucleic acid detection

Zhao

Yang

et al. 2021

Biotech & Bioengineering

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Molecular diagnosis is an essential means to detect pathogens. The portable nucleic acid detection chip has excellent prospects in places where medical resources are scarce, and it is also of research interest in the field of microfluidic chips. Here, the article developed a new type of microfluidic chip for nucleic acid detection where stretching acts as the driving force. The sample entered the chip by applying capillary force. The strain valve was opened under the action of tensile force, and the spring pump generated the power to drive the fluid to flow to the detection chamber in a specific direction. The detection of coronavirus disease 2019 (COVID‐19) was realized on the chip. The RT‐LAMP amplification system was adopted to observe the liquid color in the detection chamber to decide whether the sample tested positive or negative qualitatively.

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“…In recent decades, various portable devices based on completely different signal principles, − such as thermometers, − barometers, , pH meters, , and portable microfluidic devices, − have aroused great interest in the quantitative detection of cancer cells. Especially in the development of convenient, rapid, and point-of-care detection techniques for cancer cells, portable analysis may become an attractive and promising candidate due to its convenient operation, rapid use, low cost, and capability of on-site testing for more convenient patient monitoring and management. − With an aim toward portable detection, gas pressure-based bioassays have been successfully used for the detection of cancer cells and protein biomarkers by a pressure meter as a readout. Also, a thermal-based detection method has been used for the sensitive detection of cancer cells, small molecules, and some biomarkers , by a conventional thermometer as a signal readout.…”

mentioning

confidence: 99%

NIR II Light-Response Au Nanoframes: Amplification of a Pressure- and Temperature-Sensing Strategy for Portable Detection and Photothermal Therapy of Cancer Cells

Sun

et al. 2021

Anal. Chem.

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Quantitative detection of cancer cells using portable devices is promising for the development of simple, fast, and pointof-care cancer diagnostic techniques. However, how to further amplify the detection signal to improve the sensitivity and accuracy of detecting cancer cells by portable devices remains a challenge. To solve the problem, we, for the first time, synthesized folic-acidconjugated Au nanoframes (FA-Au NFs) with amplification of pressure and temperature signals for highly sensitive and accurate detection of cancer cells by portable pressure meters and thermometers. The resulting Au NFs exhibit excellent near-infrared (NIR) photothermal performance and catalase activity, which can promote the decomposition of NH 4 HCO 3 and H 2 O 2 to generate corresponding gases (CO 2 , NH 3 , and O 2 ), thereby synergistically amplifying pressure signals in a closed reaction vessel. At the same time, Au NFs with excellent peroxidase-like activity can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce TMB oxide (oxTMB) with a strong photothermal effect, thereby cooperating with Au NFs to amplify the photothermal signal. In the presence of cancer cells with overexpressing folate receptors (FRs), the molecular recognition signals between FA and FR can be converted into amplified pressure and temperature signals, which can be easily read by portable pressure meters and thermometers, respectively. The detection limits for cancer cells using pressure meters and thermometers are 6 and 5 cells/mL, respectively, which are better than other reported methods. Moreover, such Au NFs can improve tumor hypoxia by catalyzing the decomposition of H 2 O 2 to produce O 2 and perform photothermal therapy of cancer. Together, our work provides new insight into the application of Au NFs to develop a dual-signal sensing platform with amplification of pressure and temperature signals for portable and ultrasensitive detection of cancer cells as well as personalized cancer therapy.

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Point-of-care cancer diagnostic devices: From academic research to clinical translation

Cited by 69 publications

References 167 publications

Peptide‐Conjugated Silver Nanoparticles for the Colorimetric Detection of the Oncoprotein Mdm2 in Human Serum

Peptide‐Conjugated Silver Nanoparticles for the Colorimetric Detection of the Oncoprotein Mdm2 in Human Serum

Stretch‐driven microfluidic chip for nucleic acid detection

NIR II Light-Response Au Nanoframes: Amplification of a Pressure- and Temperature-Sensing Strategy for Portable Detection and Photothermal Therapy of Cancer Cells

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