Point-of-care breath test for biomarkers of active pulmonary tuberculosis

Phillips, Michael; Basa-Dalay, Victoria; Blais, Jaime D.; Bothamley, Graham; Chaturvedi, Anirudh; Modi, Kinjal; Pandya, Mauli; Natividad, Maria Piedad R.; Patel, Urvish; Ramraje, Nagsen N.; Schmitt, Peter A.; Udwadia, Zarir

doi:10.1016/j.tube.2012.04.002

Cited by 155 publications

(142 citation statements)

References 29 publications

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“…Notably, M. tuberculosis-associated breath volatile metabolites detected in active pulmonary TB were structurally similar but not identical to those generated from in vitro cultures (73). The results suggest the feasibility of distinguishing active pulmonary TB from nonactive TB by breath VOC patterns.…”

Section: Respiratory Infectionsmentioning

confidence: 79%

“…The second approach, which is based upon a combination of pathogen-specific biomarkers and those generated in vivo during reciprocal host-pathogen interactions, has been successfully utilized for TB detection (71)(72)(73). In the clinical studies described above, results of potential clinical value have been reported by Michael Phillips and coworkers, who evaluated the performance of TB-related breath VOC profiles on a large number of TB patients by using a convenient POC system (72,73). However, monitoring of changes/alterations in these biomarkers during treatment and their prognostic significance remain to be evaluated.…”

Section: Discussionmentioning

confidence: 99%

“…This VOC pattern in the breath of patients with active TB identified M. tuberculosis infection in this randomly selected population with an overall accuracy of 85.5%, as revealed by C-statistic values, compared to diagnosis based on sputum culture, microscopic smear, chest radiograph, and clinical symptoms (72). In a similar study, a 6-min breath testing system for POC use (Breath Link) was applied to investigate 279 individuals at 4 centers in 3 different countries representing the Philippines, the United Kingdom, and India (73). Using multiple Monte Carlo analysis, a set of biomarkers corresponding to previously reported biomarkers of active pulmonary TB were selected.…”

Section: Respiratory Infectionsmentioning

confidence: 99%

“…Using multiple Monte Carlo analysis, a set of biomarkers corresponding to previously reported biomarkers of active pulmonary TB were selected. Multivariate predictive algorithm analysis of data from 251 subjects (130 with active TB and 121 controls) allowed correct detection of active pulmonary TB with a sensitivity of 71.2%, a specificity of 72%, and an overall accuracy of 80% (73).…”

Section: Respiratory Infectionsmentioning

confidence: 99%

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Clinical Application of Volatile Organic Compound Analysis for Detecting Infectious Diseases

2013

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SUMMARY This review article introduces the significance of testing of volatile organic compounds (VOCs) in clinical samples and summarizes important features of some of the technologies. Compared to other human diseases such as cancer, studies on VOC analysis in cases of infectious diseases are limited. Here, we have described results of studies which have used some of the appropriate technologies to evaluate VOC biomarkers and biomarker profiles associated with infections. The publications reviewed include important infections of the respiratory tract, gastrointestinal tract, urinary tract, and nasal cavity. The results highlight the use of VOC biomarker profiles resulting from certain infectious diseases in discriminating between infected and healthy subjects. Infection-related VOC profiles measured in exhaled breath as well as from headspaces of feces or urine samples are a source of information with respect to disease detection. The volatiles emitted in clinical matrices may on the one hand represent metabolites of the infecting pathogen or on the other hand reflect pathogen-induced host responses or, indeed, a combination of both. Because exhaled-breath samples are easy to collect and online instruments are commercially available, VOC analysis in exhaled breath appears to be a promising tool for noninvasive detection and monitoring of infectious diseases.

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Section: Respiratory Infectionsmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Respiratory Infectionsmentioning

confidence: 99%

Section: Respiratory Infectionsmentioning

confidence: 99%

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Clinical Application of Volatile Organic Compound Analysis for Detecting Infectious Diseases

2013

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“…EU supported TMREST consortium is involved in a newly developed integrated Lab-on-Chip versatile platform for fast detection of MDR [31]. Handful of leading chemists are busy deciphering the pattern recognition of volatile organic compounds in breath or other body fluids to be able to develop electronic nose for TB [32,33].…”

Section: New Tools In the Horizonmentioning

confidence: 99%

True facets of TB diagnosis in 2012: Hypes and realities

Parida¹

2012

European Journal of Microbiology and Immunology

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Tuberculosis takes a heavy toll of ~5000 lives every day from the disease; responsible for the 86% of DALY burden. Despite having drugs to treat TB efficiently, we have failed to control the disease. Mycobacterium tuberculosis has exploited it to their advantage evolving with multiple mutations making it resistant to first-line and second-line drugs. Most of the high-burden countries are lowmedium income countries, their national TB program (NTP) still use sputum smear microscopy as the tool of diagnosis. Many new molecular tools are emerging, but confuse the larger TB clinical scientific community at the NTPs. Coherent information need to be disseminated, encouraging TB scientific community to generate evidences within NTPs assessing new tools through critical analyses in terms of value addition and cost benefit before considering rolling out in the program. It is also imperative that the scientific community need to have an open mind to use different tools in the right permutation and combination than being exclusive of one another. This article portrays an overview of the diagnostics landscape in 2012 with pros and cons of different tools to be able to generate a step-wise algorithm for optimal exploitation of the tools within available resources in each of the settings.

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Secondary Electrospray Ionization Mass Spectrometry for Breath Studies

Zhu

Hill

2018

Encyclopedia of Analytical Chemistry

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Spurred by recent events, there is an ever‐growing interest in the detection of volatile metabolites through human breath. Breath analysis, which is entirely noninvasive, can be applied to clinical diagnosis and therapeutic monitoring, where frequent sampling is required. The history of breath analysis can be traced back to as early as the time of ancient Greek. While the benefits of breath analysis are attractive, its technical basis and implementation are challenging. Despite the countless efforts in making use of breath analysis to guide clinical decisions, the potential of breath analysis remains to be fully exploited. The instruments used for breath analysis must be very selective, sensitive, and comprehensive. Comparing the published literature of several other mass spectrometry (MS)‐based breath analysis techniques, this review paper particularly focused on one recently developed breath analysis technique, secondary electrospray ionization mass spectrometry (SESI‐MS). SESI‐MS has major advantages such as its high ionization efficiency and its flexibility comparing to other existing techniques, as it can be interfaced with high‐end commercial mass spectrometers. In this review, the instrumentation, mechanism of ionization and extensive applications of SESI‐MS are discussed in detail.

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Point-of-care breath test for biomarkers of active pulmonary tuberculosis

Cited by 155 publications

References 29 publications

Clinical Application of Volatile Organic Compound Analysis for Detecting Infectious Diseases

Clinical Application of Volatile Organic Compound Analysis for Detecting Infectious Diseases

True facets of TB diagnosis in 2012: Hypes and realities

Secondary Electrospray Ionization Mass Spectrometry for Breath Studies

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