Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS

Abstract

In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawbacks that impede metabolome coverage. In this study, we aimed at developing biofluid-specific metabolome sampling membranes (MetaSAMPs) that offer a directly applicable and stabilizing substrate for AIMS. Customized rectal, salivary, and urinary MetaSAMPs consisting of electrospun (nano)fibrous membranes of blended hydrophilic (polyvinylpyrrolidone and polyacrylonitrile) and lipophilic (polystyrene) polymers supported metabolite absorption, adsorption, and desorption. Moreover, MetaSAMP demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis and was successfully validated in two pediatric cohorts (MetaBEAse, n = 234 and OPERA, n = 101). By integrating anthropometric and (patho)physiological with MetaSAMP-AIMS metabolome data, we obtained substantial weight-driven predictions and clinical correlations. In conclusion, MetaSAMP holds great clinical application potential for on-the-spot metabolic health stratification.

The 32nd Annual Conference of ECOG, Albena, Bulgaria, September 7-9 2023, Abstracts

“…Methods: A nanofibrous electrospun membrane (MetaSAMP®, WO2021/191467) 3 was developed toward holistic metabolome Background/Aims: The surveillance of childhood obesity is one of the recommended approaches in public health to tackle the current childhood obesity epidemic. The aim of the present study is to highlight the main results, trends and conclusions from the obesity surveillance among first graders in Bulgaria in the period 2008-2019.…”

“…The search was done without limiting the years of publication. The inclusion criteria included: (1) pediatric samples, (2) full text available, (3) original research articles. Articles were excluded if they were editorials, letters, replies from authors, review articles, and articles without a full text.…”

“…1 ; Lespagnol, C. 1 ; Saux, P. 2 ; Amselem, J. 3 ; Dabbas, M. 4 ; De Filippo, G. 5,6 ; Dubern, B. 7 ; Pattou, F. Background: Bariatric surgery in teenagers has been practiced in France since late 2000s in centers trained for management of severe obesity patients and under strict conditions for preparation and follow-up.…”

“…Siham Alamin 1 ; Iris Ciba 1,3 ; Banu K. Aydin 1,2 ; Hannes Manell 1,3 ; Sara Y. Cerenius 1,2 ; Ida Eriksson 2 ; Peter Bergsten 1,2,3 ; Anders Forslund 1,2,3 ; Rasmus Stenlid 1,2,3 1 Department of Women's and Children's Health, Uppsala University, SE-75185 Uppsala, Sweden; 2 Department of Medical Cell Biology, Uppsala University, SE-75123 Uppsala, Sweden; 3 Pediatric Obesity Clinic, Uppsala University Hospital, SE-75185 Uppsala, Sweden Background/Aims: Metabolic associated fatty liver disease (MAFLD) is a known complication of obesity. MAFLD may progress to hepatic steatosis, cirrhosis, and in extreme cases hepatic cell carcinoma and complete liver failure.…”

3D Printing of Succulent‐Inspired Microneedle Array for Enhanced Tissue Adhesion and Controllable Drug Release

“…[1] Currently, widely used drug delivery systems, such as implantable pumps, [2,3] electrospun nanofibers, [4][5][6] and nanoparticles, [7,8] encounter the challenge of initial burst release, as their release mechanisms largely rely on drug molecule diffusion or material degradation. Various strategies, including creating artificial gradients, [9] fine-tuning material degradability or diffusion coefficients, [10][11][12] and modifying nanoparticle surfaces, [13][14][15] have been explored to extend drug release duration. Although these solutions have improved the long-term release behavior of drug delivery systems, they usually necessitate repeated implantation surgeries or injections, resulting in patient discomfort and reduced treatment adherence.…”