Recent advances in high-throughput biofluid metabotyping by direct infusion and ambient ionization mass spectrometry

Plekhova, Vera; De Windt, Kimberly; De Spiegeleer, Margot; De Graeve, Marilyn; Vanhaecke, Lynn

doi:10.1016/j.trac.2023.117287

Cited by 4 publications

(1 citation statement)

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“…Subsequent reinjection of such a droplet population onto a suitable analytical system would allow for heterogeneity between colonies to be identified. Droplet reinjection-MS has been demonstrated in the literature by several academic groups, [33][34][35][36][37][38] identifiable via the MS data. 36 Belders' group have furthered this in several publications in which they have reinjected droplets containing different types of actinobacteria, incubated both on 39 and off-chip.…”

Section: Introductionmentioning

confidence: 99%

Assembly of Droplet Reinjection-MS Interfaces: Overcoming Droplet Coalescence for High Throughput Screening Applications.

Kempa,

Smith,

et al. 2024

Preprint

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Droplet microfluidics coupled with mass spectrometry is evolving for high throughput screening. A commercial solution that can readily interface to existing mass spectrometry instrumentation would be highly desirable and allow the technology to become accessible to those without experience in droplet microfluidics. Here, we demonstrate a droplet reinjection workflow to infuse samples into three common mass spectrometers, each with different electrospray ionisation (ESI) source configurations. The ease of chip-mass spectrometry (MS) coupling is explored and difficulties highlighted. Particular attention has been turned to ESI sources which apply the electrospray voltage directly to the incoming droplet emitter (‘push source’), as these were found to cause droplet coalescence within the microfluidic chip reinjection channel. To overcome such a difficulty, we have identified different solutions including a grounded mesh insert as a shielding approach, and minor changes to chips and ESI sources to stabilise incoming droplets. Results obtained allow the mass spectrum of individual reinjected droplets to be determined without hindrance from the electrospray voltage. The problems and solutions identified here, outline the foundations for the development of a commercial droplet reinjection-mass spectrometry interface.

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Section: Introductionmentioning

confidence: 99%

Assembly of Droplet Reinjection-MS Interfaces: Overcoming Droplet Coalescence for High Throughput Screening Applications.

Kempa,

Smith,

et al. 2024

Preprint

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Straightforward Creation of Multishell Hollow Hybrids for an Integrated Metabolic Monitoring System in Disease Management

Zhang,

Shi,

Chen

et al. 2024

Small

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Multidimensional metabolic analysis has become a new trend in establishing efficient disease monitoring systems, as the constraints associated with relying solely on a single dimension in refined monitoring are increasingly pronounced. Here, coordination polymers are employed as derivative precursors to create multishell hollow hybrids, developing an integrated metabolic monitoring system. Briefly, metabolic fingerprints are extracted from hundreds of serum samples and urine samples, encompassing not only membranous nephropathy but also related diseases, using high‐throughput mass spectrometry. With optimized algorithm and initial feature selection, the established combined panel demonstrates enhanced accuracy in both subtype differentiation (over 98.1%) and prognostic monitoring (over 95.6%), even during double blind test. This surpasses the serum biomarker panel (≈90.7% for subtyping, ≈89.7% for prognosis) and urine biomarker panel (≈94.4% for subtyping, ≈76.5% for prognosis). Moreover, after attempting to further refine the marker panel, the blind test maintains equal sensitivity, specificity, and accuracy, showcasing a comprehensive improvement over the single‐fluid approach. This underscores the remarkable effectiveness and superiority of the integrated strategy in discriminating between MN and other groups. This work has the potential to significantly advance diagnostic medicine, leading to the establishment of more effective strategies for patient management.

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3D-printing advances mass spectrometry in biomedical analysis

Chen,

Zhang,

Stevanović

et al. 2024

LabMed Discovery

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Recent advances in high-throughput biofluid metabotyping by direct infusion and ambient ionization mass spectrometry

Cited by 4 publications

References 130 publications

Assembly of Droplet Reinjection-MS Interfaces: Overcoming Droplet Coalescence for High Throughput Screening Applications.

Assembly of Droplet Reinjection-MS Interfaces: Overcoming Droplet Coalescence for High Throughput Screening Applications.

Straightforward Creation of Multishell Hollow Hybrids for an Integrated Metabolic Monitoring System in Disease Management

3D-printing advances mass spectrometry in biomedical analysis

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