Point mutation in the TGFBI gene: surface-enhanced infrared absorption spectroscopy (SEIRAS) as an analytical method

Towards multi-molecular surface-enhanced infrared absorption using metal plasmonics

“…SEIRA has been used to detect DNA, both qualitatively 75 and quantitatively. 20 To do so, the majority of SEIRA-based DNA sensors utilize functionalized surfaces, which bind a specific DNA sequence only.…”

“…a point mutation was differentiated from the wild type, based on their respective spectral signatures. 75 Synthesized gold nanoparticles covered with a layer of the analyte gene were assembled into thin films on a glass slide, where the formation of nanoparticle clusters resulted in the enhancement of the IR signal of DNA. In this manner, detection of the point mutation adenosine to guanine in the human TGFBI (transforming growth factor beta-induced) gene was enabled with 50% higher efficacy than in conventional FTIR analysis.…”