pO157_Sal, a Novel Conjugative Plasmid Detected in Outbreak Isolates of Escherichia coli O157:H7

Wang, Ping; Xiong, Yanwen; Lan, Ruiting; Ye, Changyun; Wang, Hua; Ren, Jun; Jing, Huaiqi; Wang, Yiting; Zhou, Zhemin; Cui, Zhigang; Zhao, Hongqing; Chen, Yuli; Jin, Dong; Bai, Xuemei; Zhao, Ailan; Wang, Yan; Zhang, Shaomin; Sun, Hui; Li, Juan; Wang, Tao; Wang, Lei; Xu, Jianguo

doi:10.1128/jcm.02530-10

Cited by 21 publications

(23 citation statements)

References 30 publications

(25 reference statements)

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“…There are several reports associated with outbreaks of STEC around the world. In a massive outbreak in China, 195 hospitalised HUS patients and 177 deaths have been reported (Wang et al, 2011;Xiong et al, 2012). In another outbreak caused by non-O157 STEC, infected cases including 43 deaths have been recently reported in the European Union (ECDC, 2011).…”

Section: Introductionmentioning

confidence: 99%

Virulence gene profiles of Shiga-toxin producing Escherichia coli isolates from retail raw meat in Iran.

Panahee¹,

Pourtaghi²

2017

BJVM

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Shiga toxin-producing Escherichia coli (STEC) is recognised as toxin producing group of E. coli and one of the most significant foodborne pathogens worldwide. The aim of this study was to detect STEC and determine virulence gene profiles of these pathogens in different kinds of meat and products in Iran. For this reason a total of 182 samples of minced beef, mutton, chicken meat, chicken feet and mechanically separated chicken meat were collected from retail markets for detection of STEC by PCR method. Of the 72 E. coli isolated from examined samples, 29 of them were STEC. The highest presence of STEC was detected in minced beef (23.5%) followed by chicken feet (15%), mutton (13.3%), mechanically separated chicken meat (12.5%) and chicken meat (5.5%) respectively. In addition the results of PCR assay indicated that 21 (72.4%) and 4 (13.7%) of isolates carried stx2 and eaeA genes respectively. However, according to the results stx2 was the most prevalent gene detected in all kinds of examined samples. Our findings showed that evaluation of the prevalence and virulence factors of this pathogen seems necessary considering the increasing importance of STEC as one of the most significant foodborne pathogens.

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Section: Introductionmentioning

confidence: 99%

Virulence gene profiles of Shiga-toxin producing Escherichia coli isolates from retail raw meat in Iran.

Panahee¹,

Pourtaghi²

2017

BJVM

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“…The novel plasmid pO157_Sal in Xuzhou21 has been reported previously by our group [24], and this plasmid was only observed in ST96, ST23 and ST98 strains [24]. pO157_Sal contains no known virulence genes [24].…”

Section: Resultsmentioning

confidence: 67%

“…pO157_Sal contains no known virulence genes [24]. Interestingly, a similar plasmid (pEC4115) has also been found in EC4115 [22].…”

Section: Resultsmentioning

confidence: 94%

A Novel Escherichia coli O157:H7 Clone Causing a Major Hemolytic Uremic Syndrome Outbreak in China

et al. 2012

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An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7.

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“…It directly transports toxigenic proteins, but also mediates transportation at the genetic level via bacterial binding, transmits toxigenic genes, enhances pathogen virulence and contributes to bacterial evolution (17)(18)(19)(20). In previous studies, a novel subtype of T4SS known as the type IVC secretion system has been observed (17,20).…”

Section: Discussionmentioning

confidence: 99%

Preliminary screening of type IV secretion system in divergent geographic sources of Clostridiumï¿½difficile

Jia

Yang

et al. 2017

Exp Ther Med

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Abstract. In the present study, different geographical sources and sequence types (STs) of Clostridium difficile were preliminarily screened to investigate the distribution profiles of three core genes, VirB4, VirB6 and VirD4, of the type IV secretion system (T4SS). A total of 37 C. difficile strains from different sources were screened, inoculated and prepared for genome extraction. C. difficile toxins A and B were subjected to identification and multilocus sequence typing (MLST) analysis. The T4SS gene then underwent polymerase chain reaction amplification and sequencing analysis. Of the 37 strains, 25 were toxin A-and toxin B-positive, and 12 were toxin A-negative and toxin B-positive. MLST detected 11 strains with ST37, 10 with ST2, 6 with ST35, 7 with ST3, 1 with ST54, 1 with ST1 and 1 with ST119. The detection rates of VirB4, VirB6 and VirD4 were all 100% in colonies exhibiting T4SS. Single nucleotide polymorphisms (SNPs) were detected in a minority of strains. C. difficile strains with identical STs shared the same SNP loci for T4SS, and those with different STs had different SNP loci. The results of the present study may provide evidence for subsequent identification of T4SS distribution, epidemiological investigations, polymorphism analyses and research into the association between T4SS, cytotoxicity and enterotoxication in C. difficile.

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pO157_Sal, a Novel Conjugative Plasmid Detected in Outbreak Isolates of Escherichia coli O157:H7

Cited by 21 publications

References 30 publications

Virulence gene profiles of Shiga-toxin producing Escherichia coli isolates from retail raw meat in Iran.

Virulence gene profiles of Shiga-toxin producing Escherichia coli isolates from retail raw meat in Iran.

A Novel Escherichia coli O157:H7 Clone Causing a Major Hemolytic Uremic Syndrome Outbreak in China

Preliminary screening of type IV secretion system in divergent geographic sources of Clostridiumï¿½difficile

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