PM2.5 from a broiler breeding production system: The characteristics and microbial community analysis

Dai, Pengyuan; Shen, Dan; Tang, Qian; Huang, Kai; Li, Chunmei

doi:10.1016/j.envpol.2019.113368

Cited by 34 publications

(36 citation statements)

References 72 publications

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“…Typical genera found in broiler feces are Bacteriodes, Brevibacterium, Corynebacterium, Enterococcus, Lactobacillus and Staphylococcus [22– 24, 36, 37]. These taxa were also identified in our air sample microbiomes of the poultry houses as well as in other studies using different detection methods [16–18]. That suggests that the air microbiome in poultry houses can be used as a proxy for the fecal microbiome.…”

Section: Discussionsupporting

confidence: 71%

“…Not unexpected, Brachybacterium spp. have also been identified in the dust from poultry farms [18]. They have been rarely identified as pathogenic agents in humans [40].…”

Section: Discussionmentioning

confidence: 99%

“…animal production facilities, slaughter houses or in the food industry airborne pathogens and their resistome might cause health concerns for the humans working in such spaces [17, 48]. Likewise, the animals in these facilities are also exposed to the same potential pathogens that are present in the air [18, 49]. With the increasing availability of shotgun metagenomics, it becomes possible to monitor animal house facilities for a wide variety of pathogens without the limits in detection when using cultivation or dedicated PCR approaches.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, Dai et al, showed that a range of potentially pathogenic bacterial genera, such as Corynebacterium, Escherichia and Staphylococcus are present in Chinese poultry house air [9]. Most of these organisms are associated to particulate matter particles present in the air with a particle size diameter smaller than 2.5 µm fraction [18]. Other studies using 16s rRNA DGGE profiling [16], shotgun sequencing [19], or cultivation [17] from material collected on air filters showed similar organisms being present in Canadian or Polish poultry houses.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Campylobacter in air samples from poultry houses using shot-gun metagenomics – a pilot study

Haverkamp

Spilsberg

et al. 2021

Preprint

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Background: Foodborne pathogens such as Campylobacter jejuni are responsible for a large fraction of the gastrointestinal infections worldwide associated with poultry meat. Campylobacter spp. can be found in the chicken fecal microbiome and can contaminate poultry meat during the slaughter process. The current standard methods to detect these pathogens at poultry farms use fecal dropping or boot swaps in combination with cultivation / PCR. In this study, we have used air filters in combination with shotgun metagenomics for the detection of Campylobacter in poultry houses and MOCK communities to test the applicability of this approach for the detection of foodborne pathogens. Results: The spiked MOCK communities showed that we could detect as little as 200 CFU Campylobacter per sample using our protocols. Since we were interested in detecting Campylobacter, a DNA extraction protocol for Gram negative bacteria was chosen, and as expected, we found that the DNA extraction protocol created a substantial bias affecting the community composition of the MOCK communities. It can be expected that the same bias is present for poultry house samples analyzed. We observed significant amounts of Campylobacter on the air filters using both real-time PCR as well as shotgun metagenomics, irrespective of the amount of spiked in Campylobacter cells, suggesting that the flocks in both houses harboured Campylobacter spp.. Interestingly, in both houses we find diverse microbial communities present in the indoor air. In addition, have we tested the Campylobacter detection rate using shotgun metagenomics by spiking with different levels of C. jejuni cells in both the mock and the house samples. This showed that even with limited sequencing Campylobacter is detectable in samples with low abundance. Conclusions: These results show that air sampling of poultry houses in combination with shotgun metagenomics can detect and identify Campylobacter spp. present at low levels. This is important since early detection of Campylobacter in food production can help to decrease the number of food-borne infections.

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Section: Discussionsupporting

confidence: 71%

“…Not unexpected, Brachybacterium spp. have also been identified in the dust from poultry farms [18]. They have been rarely identified as pathogenic agents in humans [40].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Campylobacter in air samples from poultry houses using shot-gun metagenomics – a pilot study

Haverkamp

Spilsberg

et al. 2021

Preprint

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“…Meanwhile, many pathogenic bacteria can often be detected in the air near these farms. The main pathogenic bacteria in the atmosphere of farms include Bacillus, Corynebacterium, and Streptococcus (McEachran et al, 2015;Skora et al, 2016;Dai et al, 2020), which are mainly derived from animal excrement and feed or are related to the farming activities in the farm (Zhang et al, 2019).…”

Section: Anthropogenic Sourcesmentioning

confidence: 99%

The source and transport of bioaerosols in the air: A review

Xie

Bai

et al. 2020

Front. Environ. Sci. Eng.

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Recent pandemic outbreak of the corona-virus disease 2019 (COVID-19) has raised widespread concerns about the importance of the bioaerosols. They are atmospheric aerosol particles of biological origins, mainly including bacteria, fungi, viruses, pollen, and cell debris. Bioaerosols can exert a substantial impact on ecosystems, climate change, air quality, and public health. Here, we review several relevant topics on bioaerosols, including sampling and detection techniques, characterization, effects on health and air quality, and control methods. However, very few studies have focused on the source apportionment and transport of bioaerosols. The knowledge of the sources and transport pathways of bioaerosols is essential for a comprehensive understanding of the role microorganisms play in the atmosphere and control the spread of epidemic diseases associated with them. Therefore, this review comprehensively summarizes the up to date progress on the source characteristics, source identification, and diffusion and transport process of bioaerosols. We intercompare three types of diffusion and transport models, with a special emphasis on a widely used mathematical model. This review also highlights the main factors affecting the source emission and transport process, such as biogeographic regions, land-use types, and environmental factors. Finally, this review outlines future perspectives on bioaerosols.

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The possible role of particulate matter on the respiratory microbiome: evidence from in vivo to clinical studies

et al. 2023

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PM2.5 from a broiler breeding production system: The characteristics and microbial community analysis

Cited by 34 publications

References 72 publications

Detection of Campylobacter in air samples from poultry houses using shot-gun metagenomics – a pilot study

Detection of Campylobacter in air samples from poultry houses using shot-gun metagenomics – a pilot study

The source and transport of bioaerosols in the air: A review

The possible role of particulate matter on the respiratory microbiome: evidence from in vivo to clinical studies

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