“Plus-C” odorant-binding protein genes in two Drosophila species and the malaria mosquito Anopheles gambiae

Crystal and Solution Studies of the “Plus-C” Odorant-binding Protein 48 from Anopheles gambiae

“…More than 60 OBP-encoding sequences have been found in the A. gambiae genome (7)(8)(9). These proteins have been classified into three different groups based on primary protein sequence features as follows: "classic" OBPs with a typical six-cysteine signature; "atypical" OBPs, and Plus-C OBPs (8,10,11).…”

“…The Plus-C OBPs were first reported in Drosophila and Anopheles (9,17) and recently to the pea aphid Acyrthosiphon pisum (Hemiptera) (18) indicating that protein evolution occurred before the divergence of diptera from other species; therefore, these proteins may also be present in other insect orders. The Anopheles gambiae Plus-C OBPs are distinguished from the other classes by their longer sequences and the presence of 12 cysteines in conserved positions, six of which correspond closely to the conserved cysteines residues of the classic OBPs (8,19).…”

Characterisation of Bombyx mori Odorant-binding Proteins Reveals that a General Odorant-binding Protein Discriminates Between Sex Pheromone Components

“…11,12,13 This sequence motif has been used for genome-wide identification and annotation of OBP genes in a range of insect species. 14,15,16,17 Based on their sequence and their expression patterns in male and female antennae, OBPs in lepidopteran species are usually divided into different subfamilies; the PBP 18 , the GOBP 9,18,19 and the antennal binding protein X homologues (ABPx). 20 PBPs of Lepidoptera are either specific to, or highly enriched in, the antennae of male moths and have been shown to bind the sex pheromones produced by females.…”

Review An overview of odorant-binding protein functions in insect peripheral olfactory reception

“…Cysteine residue Other description Example Classical OBPs 6 3 disulfide bonds formed by BmorPBP 6 cysteine residues (Vogt and Riddiford, 1981) ~14 kDa Plus-C OBPs 6 + 2/3 1 highly conserved proline residue AgamOBP48 Putative Plus-C alignment GenBank ® at least 2 conserved cysteines accession No., ALIGN_000581 (Zhou et al, 2004a) Adaptive evolution in diverse habitats with diverse odor molecules has resulted in a large OBP family with variant proteins (Hekmat-Scafe et al, 2002). Most of insect-OBP sequences are divergent among different orders, even less than 20% identity from different insect genera (Zhou et al, 2010a).…”