Pluripotent pig embryonic stem cell lines originating from in vitro-fertilized and parthenogenetic embryos

Choi, Kwang‐Hwan; Lee, Dong‐Kyung; Oh, Jong-Nam; Kim, Seung‐Hun; Lee, Mingyun; Woo, Sang-Ho; Kim, Dae-Yong; Lee, Chang Kyu

doi:10.1016/j.scr.2020.102093

Cited by 12 publications

(5 citation statements)

References 5 publications

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“…Comparative genome-wide and epigenome-wide analysis of host animal pluripotency with human iPS cells can provide critical guidance. Besides human pluripotency maintenance in human iPSCs, ES cells have also recently been derived from large animals such as pigs, cows, and sheep ( Bogliotti et al, 2018 ; Choi et al, 2020 ; Vilarino et al, 2020 ; Kumar et al, 2021 ). However, except for one study involving pig blastomeres, it has been technically challenging to accurately define naïve state pluripotency and germline competence of large-animal stem cells ( Matsunari et al, 2013 ).…”

Section: Future Perspectivesmentioning

confidence: 99%

Towards human organ generation using interspecies blastocyst complementation: Challenges and perspectives for therapy

Sarmah

Sawada

Hwang

et al. 2023

Front. Cell Dev. Biol.

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Millions of people suffer from end-stage refractory diseases. The ideal treatment option for terminally ill patients is organ transplantation. However, donor organs are in absolute shortage, and sadly, most patients die while waiting for a donor organ. To date, no technology has achieved long-term sustainable patient-derived organ generation. In this regard, emerging technologies of chimeric human organ production via blastocyst complementation (BC) holds great promise. To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology.

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Section: Future Perspectivesmentioning

confidence: 99%

Towards human organ generation using interspecies blastocyst complementation: Challenges and perspectives for therapy

Sarmah

Sawada

Hwang

et al. 2023

Front. Cell Dev. Biol.

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“…Chakritbudsabong et al also reported the generation of pig embryonic fibroblast-derived iPSCs and their differentiation ability into cardiomyocytes [79]. Choi et al reported the generation of pluripotent pig ESCs derived from in vitro-fertilized and parthenogenetic embryos [80]. Although iPSC generation from various animal species has been attempted and criticized [81], it will largely contribute to the autologous/allogeneic transplantation experiments in the future, which may further promote stem cell-based cardiovascular regenerative medicine.…”

Section: Allogeneic Transplantation Models For Cardiovascular Regener...mentioning

confidence: 99%

Large Animal Models in Cardiovascular Research

Osada¹,

Murata²,

Masumoto³

2023

Animal Models and Experimental Research in Medicine

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Studies of not only preclinical cardiovascular research but also those of life science, medical, and pharmacological fields commonly utilize small animal models. However, for the advancement of cardiovascular medicine, researches using large animal models are important step for preclinical validation of therapeutic efficacy and safety by virtue of having models with a body and heart size comparable with that of a human, providing clinically relevant experiments without the concern of over- or under-estimating therapeutic effects and risks. In particular, pigs are considered as a suitable animal model for research in cardiovascular medicine because of the similarities in physiology, metabolism, genomics, and proteomics to those in humans. Another advantage of pigs is the availability of various heart disease models such as myocardial infarction and genetically established cardiomyopathy. The present review updates the contributions of large animal model-based research to the development of cardiovascular medicine, especially focusing on the utility of pig models.

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“…ESCs have been successfully isolated from a variety of organisms including humans, mice, chickens [58], fish [59], and cows [60]. ESCs can also be obtained from embryos formed by in vitro fertilization which is one of the options for acquiring pig ESCs [61] which are otherwise difficult to isolate, as discussed below.…”

Section: Embryonic Stem Cells (Escs)mentioning

confidence: 99%

Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives

et al. 2022

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Global food systems are under significant pressure to provide enough food, particularly protein-rich foods whose demand is on the rise in times of crisis and inflation, as presently existing due to post-COVID-19 pandemic effects and ongoing conflict in Ukraine and resulting in looming food insecurity, according to FAO. Cultivated meat (CM) and cultivated seafood (CS) are protein-rich alternatives for traditional meat and fish that are obtained via cellular agriculture (CA) i.e., tissue engineering for food applications. Stem and progenitor cells are the building blocks and starting point for any CA bioprocess. This review presents CA-relevant vertebrate cell types and procedures needed for their myogenic and adipogenic differentiation since muscle and fat tissue are the primary target tissues for CM/CS production. The review also describes existing challenges, such as a need for immortalized cell lines, or physical and biochemical parameters needed for enhanced meat/fat culture efficiency and ways to address them.

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Pluripotent pig embryonic stem cell lines originating from in vitro-fertilized and parthenogenetic embryos

Cited by 12 publications

References 5 publications

Towards human organ generation using interspecies blastocyst complementation: Challenges and perspectives for therapy

Towards human organ generation using interspecies blastocyst complementation: Challenges and perspectives for therapy

Large Animal Models in Cardiovascular Research

Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives

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