PLLA/HA Electrospin Hybrid Nanofiber Scaffolds: Morphology, In Vitro Degradation and Cell Culture Potential

Abstract

Electrospinning has recently emerged as a potential technique for fabricating biomimetic tissue engineering scaffolds. In this study, Poly (l-lactic acid) (PLLA) /Nano-hydroxyapatite (HA) hybrid nanofibers scaffolds were prepared by electrospinning. The relationship between process parameters and fiber diameter has been investigated. The fiber diameter decreased with decreasing polymer concentration and with increasing electrospinning voltage; After 6 weeks of in vitro degradation, the mass, viscosity-average molecular weight of the nanofibers scaffolds and the pH value of the degradation solution were changed, the fibers lost their surface smoothness and a regular rough topology was generated after 32d of degradation, the degradation rates of PLLA/HA hybrid nanofibers were slower than those of pure PLLA fibers; The biocompatibility of the nanofibers scaffold has also been investigated by culturing cells on the nanofibers scaffold, elementary results showed that the cells adhered and proliferated well on the PLLA/HA hybrid nanofibers scaffolds.

Structural stability and sustained release of protein from a multilayer nanofiber/nanoparticle composite

“…Similar results were reported by Wang and Norouzi et al for emulsion electrospinning [30,31]. containing chitosan nanoparticles was attributed only to chitosan degradation [34,[49][50][51]. As shown in Fig.…”

Accelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity

“…It was worth noting that the degradation rate of scaffold was greatly accelerated with the increase of PGA. Specifically, the 1PLLA/1PGA/HAP scaffold had 25.0 ± 4.2% weight loss, which was a little lower than that of the poly (D,L lactide-coglycolide (PLGA)/HAP scaffold or composite [ 45 , 46 ]. The results indicated that PGA regulated the degradation rate of scaffolds, which could be attributed to the fact that PGA's strong hydrophilicity leaded to self-degradation and increased the contact area between water molecules and PLLA chains, thus catalyzed the hydrolysis of PLLA.…”

Electrospun nanostructured scaffolds for bone tissue engineering

“…Bone-like nHA/collagen composites have been developed previously by mineralizing the type I collagen sheet [13,14], though with mechanical properties that are too weak for practical applications. HA, though brittle by itself, has been used for bone regeneration and biomedical implant applications due to its biodegradability, bioactivity and osteoconductive properties [15]. Therefore, biocomposite nanofibers containing HA fabricated by electrospinning might be suitable as a scaffold with improved biological properties for bone tissue engineering.…”