Electrospun nanostructured scaffolds for bone tissue engineering

Prabhakaran, Molamma P.; Venugopal, Jayarama Reddy; Ramakrishna, Seeram

doi:10.1016/j.actbio.2009.05.007

Cited by 392 publications

(232 citation statements)

References 39 publications

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“…The red stains (bone nodules) were ARS-calcium chelating products. 48 As shown in Figure 8B, the cells cultured on PEG/PLA fibrous scaffolds clearly showed matrix mineralization with more intense ARS staining than the control. To quantify mineralization, constructs were desorbed using 10% cetylpyridinium chloride for 1 hour, and the absorbance of the solution was measured at 570 nm (n = 3).…”

Section: Ars Staining and Mineralization Quantificationmentioning

confidence: 93%

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

Fu²,

Fan³

et al. 2011

IJN

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Polylactide (PLA) electrospun fibers have been reported as a scaffold for bone tissue engineering application, however, the great hydrophobicity limits its broad application. In this study, the hybrid amphiphilic poly(ethylene glycol) (PEG)/hydrophobic PLA fibrous scaffolds exhibited improved morphology with regular and continuous fibers compared to corresponding blank PLA fiber mats. The prepared PEG/PLA fibrous scaffolds favored mesenchymal stem cell (MSC) attachment and proliferation by providing an interconnected porous extracellular environment. Meanwhile, MSCs can penetrate into the fibrous scaffold through the interstitial pores and integrate well with the surrounding fibers, which is very important for favorable application in tissue engineering. More importantly, the electrospun hybrid PEG/PLA fibrous scaffolds can enhance MSCs to differentiate into bone-associated cells by comprehensively evaluating the representative markers of the osteogenic procedure with messenger ribonucleic acid quantitation and protein analysis. MSCs on the PEG/PLA fibrous scaffolds presented better differentiation potential with higher messenger ribonucleic acid expression of the earliest osteogenic marker Cbfa-1 and mid-stage osteogenic marker Col I . The significantly higher alkaline phosphatase activity of the PEG/PLA fibrous scaffolds indicated that these can enhance the differentiation of MSCs into osteoblast-like cells. Furthermore, the higher messenger ribonucleic acid level of the late osteogenic differentiation markers OCN ( osteocalcin ) and OPN ( osteopontin ), accompanied by the positive Alizarin red S staining, showed better maturation of osteogenic induction on the PEG/PLA fibrous scaffolds at the mineralization stage of differentiation. After transplantation into the thigh muscle pouches of rats, and evaluating the inflammatory cells surrounding the scaffolds and the physiological characteristics of the surrounding tissues, the PEG/PLA scaffolds presented good biocompatibility. Based on the good cellular response and excellent osteogenic potential in vitro, as well as the biocompatibility with the surrounding tissues in vivo, the electrospun PEG/PLA fibrous scaffolds could be one of the most promising candidates in bone tissue engineering.

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Section: Ars Staining and Mineralization Quantificationmentioning

confidence: 93%

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

Fu²,

Fan³

et al. 2011

IJN

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“…Further, in a subcutaneous model, none of the substrates induced cellular orientation parallel to the direction of the substrate topography. It is tempting to hypothesise that two-dimensional imprinted substrates are overwhelmed with body fluids and protein adsorption upon implantation, prohibiting favourable cell / material interaction at the substrate-tissue nano-biointerface and that three-dimensional fibrous constructs are more effective for directional neural [77][78][79], tendon [29,35,80], bone [81][82][83] and skin [84][85][86] neotissue formation and promote relatively enhanced cell growth, motility, matrix deposition and neotissue growth through the provision of a true three-dimensional environment.…”

Section: Discussionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractControlling the cell-substrate interactions at the bio-interface is becoming an inherent element in the design of implantable devices. Modulation of cellular adhesion in vitro, through topographical cues, is a well-documented process that offers control over subsequent cellular functions. However, it is still unclear whether surface topography can be translated into a clinically functional response in vivo at the tissue / device interface. Herein, we demonstrated that anisotropic substrates with a groove depth of ~317 nm and ~1,988 nm promoted human tenocyte alignment parallel to the underlying topography in vitro. However, the rigid poly(lactic-co-glycolic acid) substrates used in this study upregulated the expression of chondrogenic and osteogenic genes, indicating possible tenocyte trans-differentiation. Of significant importance is that none of the topographies assessed (~37 nm, ~317 nm and ~1,988 nm groove depth) induced extracellular matrix orientation parallel to the substrate orientation in a rat patellar tendon model. These data indicate that two-dimensional imprinting technologies are useful tools for in vitro cell phenotype maintenance, rather than for organised neotissue formation in vivo, should multifactorial approaches that consider both surface topography and substrate rigidity be established.

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“…Prabhakaran et al fabricated biocomposite polymeric nanofibers containing nano HA by electrospinning for bone tissue engineering. They suggested that the biocomposite PLLA/collagen/HA nanofibrous scaffold could improve the proliferation and mineralization of osteoblasts, resulting in the enhancement of bone regeneration [71]. Recently, Wang et al successfully fabricated electrospun fibrous scaffold with well-tailored architectures and patterns from PCL using a stainless steel mesh as the template collector [72].…”

Section: Application Of Electrospun Fibrous Materials In Tissue Enginmentioning

confidence: 99%

Electrospun nanofibrous materials for tissue engineering and drug delivery

Cui

Zhou

Chang

2010

Science and Technology of Advanced Materials

443

206

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The electrospinning technique, which was invented about 100 years ago, has attracted more attention in recent years due to its possible biomedical applications. Electrospun fibers with high surface area to volume ratio and structures mimicking extracellular matrix (ECM) have shown great potential in tissue engineering and drug delivery. In order to develop electrospun fibers for these applications, different biocompatible materials have been used to fabricate fibers with different structures and morphologies, such as single fibers with different composition and structures (blending and core-shell composite fibers) and fiber assemblies (fiber bundles, membranes and scaffolds). This review summarizes the electrospinning techniques which control the composition and structures of the nanofibrous materials. It also outlines possible applications of these fibrous materials in skin, blood vessels, nervous system and bone tissue engineering, as well as in drug delivery.

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Electrospun nanostructured scaffolds for bone tissue engineering

Cited by 392 publications

References 39 publications

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

Electrospun nanofibrous materials for tissue engineering and drug delivery

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