PLGA nanofiber membranes loaded with epigallocatechin-3-O-gallate are beneficial to prevention of postsurgical adhesions

Abstract

This study concentrates on the development of biodegradable nanofiber membranes with controlled drug release to ensure reduced tissue adhesion and accelerated healing. Nanofibers of poly(lactic- co -glycolic acid) (PLGA) loaded with epigallocatechin-3- O -gallate (EGCG), the most bioactive polyphenolic compound in green tea, were electrospun. The physicochemical and biomechanical properties of EGCG-releasing PLGA (E-PLGA) nanofiber membranes were characterized by atomic force microscopy, EGCG release and degradation profiles, and tensile testing. In vitro antioxidant activity and hemocompatibility were evaluated by measuring scavenged reactive oxygen species levels and activated partial thromboplastin time, respectively. In vivo antiadhesion efficacy was examined on the rat peritonea with a surgical incision. The average fiber diameter of E-PLGA membranes was approximately 300–500 nm, which was almost similar to that of pure PLGA equivalents. E-PLGA membranes showed sustained EGCG release mediated by controlled diffusion and PLGA degradation over 28 days. EGCG did not adversely affect the tensile strength of PLGA membranes, whereas it significantly decreased the elastic modulus and increased the strain at break. E-PLGA membranes were significantly effective in both scavenging reactive oxygen species and extending activated partial thromboplastin time. Macroscopic observation after 1 week of surgical treatment revealed that the antiadhesion efficacy of E-PLGA nanofiber membranes was significantly superior to those of untreated controls and pure PLGA equivalents, which was comparable to that of a commercial tissue-adhesion barrier. In conclusion, the E-PLGA hybrid nanofiber can be exploited to craft strategies for the prevention of postsurgical adhesions.

Antiadhesive Nanofibrous Materials for Medicine: Preventing Undesirable Tissue Adhesions

“…The efficacy of EGCG-releasing PLGA (E-PLGA) nanofiber membranes compared to those of pure PLGA and Interceed was tested in vivo in rat models. The results show that E-PLGA has a significant antiadhesive effect compared to the control and pure PLGA materials and that it gives values close to that of the commercial barrier Interceed …”

“…Natural Substances. Shin et al 22 produced PLGAbased nanofiber membranes that provide a controlled release of polyphenol epigallocatechin-3-O-gallate (EGCG) to prevent tissue adhesion and accelerate the healing process. The PLGA was electrospun with EGCG from a solution via needle electrospinning.…”

“…The results show that E-PLGA has a significant antiadhesive effect compared to the control and pure PLGA materials and that it gives values close to that of the commercial barrier Interceed. 22 Chen et al 23 aimed to prevent peritendinous adhesion with PCL nanofibers grafted with chitosan. In the study, the PCL solution was electrospun and further treated with hydrophilic plasma grafted with chitosan molecules (CS).…”

Prevention of Postsurgical Abdominal Adhesion Using Electrospun TPU Nanofibers in Rat Model

“…Macroscopic scoring of postsurgical adhesion for rats treated with PLGA nanofiber membranes loaded with epigallocatechin-3-O-gallate after a week in another study showed acceptable antiadhesion efficacy, better than untreated rats or the PLGA nanofiber-applied group. However, this nanofiber was still not a better commercial tissue adhesion barrier [ 45 ].…”

Polyphenols-loaded electrospun nanofibers in bone tissue engineering and regeneration

“…[ 29 ]. Further, the nanofibers can demonstrate a sustained drug release preserving the bioavailability of active drugs like polyphenols [ 4 , 34 ], antibiotics [ 35 ], oligopeptides [ 36 ], medicative ingredients [ 37 ], and growth factors [ 38 ]. Various drug loading strategies lead to different kinds of interaction between drugs and nanofibers, observing different drug-releasing kinetics [ 39 ].…”