Plesiomonas shigelloides sipD mutant, generated by an efficient gene transfer system, is less invasive

Xi, Daoyi; Jing, Fuyi; Liu, Qian; Cao, Bihua

doi:10.1016/j.mimet.2019.02.017

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…Cetobacterium is an important beneficial bacteria in the gut of aquatic animals, and it can produce large quantities of vitamin B-12 ( Tsuchiya et al., 2008 ). Members of the genus Plesiomonas are ubiquitous opportunistic pathogens in aquaculture systems, and can cause infections in humans ( Ekundayo and Okoh, 2019 ; Xi et al., 2019 ). In the present study, diets containing B. amyloliquefaciens increased the proportion of beneficial bacteria and decreased the proportion of harmful bacteria in yellow catfish.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary Bacillus amyloliquefaciens on the growth, immune responses, intestinal microbiota composition and disease resistance of yellow catfish, Pelteobagrus fulvidraco

Xue

Wu²,

Hong³

et al. 2022

Front. Cell. Infect. Microbiol.

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The influence of dietary probiotic Bacillus amyloliquefaciens on the growth performance, digestive enzyme activity, immune parameters and disease resistance of yellow catfish (Pelteobagrus fulvidraco) was evaluated. Commercial diet (C) or diet containing 106 cfu/g B. amyloliquefaciens (T) was fed for 4 weeks, and final weight (FW), specific growth rate (SGR) and feed conversion ratio (FCR) were improved (p<0.05) in the T group. Dietary B. amyloliquefaciens increased protease and amylase activities in the digestive tract after 2 and 4 weeks, respectively. Respiratory burst (RB), plasma lysozyme (LZM) activity, total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity were also elevated (p<0.05). Immune-related genes signal transducer and activator of transcription 1 (STATA-1), immunoglobulin M (IgM) and C-type lectin (CTL) were upregulated (p<0.05), but interleukin-1 β (IL-1β) was not (p >0.05). Intestinal microbiota analysis showed that the community structure was significantly different between the two groups; the relative abundance of Cetobacterium was increased but Plesiomonas was decreased in T. Moreover, challenge tests showed that the resistance of fish fed B. amyloliquefaciens against Aeromonas veronii and Edwardsiella ictaluri was significantly enhanced (p<0.05). In conclusion, dietary supplementation of B. amyloliquefaciens can effectively improve the growth performance, digestive enzyme activity, immune responses, intestinal microbiota composition and disease resistance of yellow catfish.

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Section: Discussionmentioning

confidence: 99%

Effects of dietary Bacillus amyloliquefaciens on the growth, immune responses, intestinal microbiota composition and disease resistance of yellow catfish, Pelteobagrus fulvidraco

Xue

Wu²,

Hong³

et al. 2022

Front. Cell. Infect. Microbiol.

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“…In this study, the suicide vector pRE112 was used to make deletion mutations of the rpoN gene and the T2SS-2 cluster of P. shigelloides [ 77 ]. The complementation strain, Δ rpoN/ pBAD33- rpoN + , was constructed by introducing the recombinant vector pBAD33- rpoN + into the Δ rpoN strain via electroporation.…”

Section: Methodsmentioning

confidence: 99%

RpoN is required for the motility and contributes to the killing ability of Plesiomonas shigelloides

Yan

Guo

et al. 2022

BMC Microbiol

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Background RpoN, also known as σ54, first reported in Escherichia coli, is a subunit of RNA polymerase that strictly controls the expression of different genes by identifying specific promoter elements. RpoN has an important regulatory function in carbon and nitrogen metabolism and participates in the regulation of flagellar synthesis, bacterial motility and virulence. However, little is known about the effect of RpoN in Plesiomonas shigelloides. Results To identify pathways controlled by RpoN, RNA sequencing (RNA-Seq) of the WT and the rpoN deletion strain was carried out for comparison. The RNA-seq results showed that RpoN regulates ~ 13.2% of the P. shigelloides transcriptome, involves amino acid transport and metabolism, glycerophospholipid metabolism, pantothenate and CoA biosynthesis, ribosome biosynthesis, flagellar assembly and bacterial secretion system. Furthermore, we verified the results of RNA-seq using quantitative real-time reverse transcription PCR, which indicated that the absence of rpoN caused downregulation of more than half of the polar and lateral flagella genes in P. shigelloides, and the ΔrpoN mutant was also non-motile and lacked flagella. In the present study, the ability of the ΔrpoN mutant to kill E. coli MG1655 was reduced by 54.6% compared with that of the WT, which was consistent with results in RNA-seq, which showed that the type II secretion system (T2SS-2) genes and the type VI secretion system (T6SS) genes were repressed. By contrast, the expression of type III secretion system genes was largely unchanged in the ΔrpoN mutant transcriptome and the ability of the ΔrpoN mutant to infect Caco-2 cells was also not significantly different compared with the WT. Conclusions We showed that RpoN is required for the motility and contributes to the killing ability of P. shigelloides and positively regulates the T6SS and T2SS-2 genes.

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“…In this study, an effective and precise conjugate transfer process mediated by the suicide vector pRE112 was used to make deletion mutations in the arcA gene of P. shigelloides [ 65 ]. The complementation strains was constructed by introducing the recombinant vector pBAD33- arcA + into the Δ arcA strain via electroporation.…”

Section: Methodsmentioning

confidence: 99%

The effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides

Yan

Guo

et al. 2021

BMC Microbiol

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Background The anoxic redox control binary system plays an important role in the response to oxygen as a signal in the environment. In particular, phosphorylated ArcA, as a global transcription factor, binds to the promoter regions of its target genes to regulate the expression of aerobic and anaerobic metabolism genes. However, the function of ArcA in Plesiomonas shigelloides is unknown. Results In the present study, P. shigelloides was used as the research object. The differences in growth, motility, biofilm formation, and virulence between the WT strain and the ΔarcA isogenic deletion mutant strain were compared. The data showed that the absence of arcA not only caused growth retardation of P. shigelloides in the log phase, but also greatly reduced the glucose utilization in M9 medium before the stationary phase. The motility of the ΔarcA mutant strain was either greatly reduced when grown in swim agar, or basically lost when grown in swarm agar. The electrophoretic mobility shift assay results showed that ArcA bound to the promoter regions of the flaK, rpoN, and cheV genes, indicating that ArcA directly regulates the expression of these three motility-related genes in P. shigelloides. Meanwhile, the ability of the ΔarcA strain to infect Caco-2 cells was reduced by 40%; on the contrary, its biofilm formation was enhanced. Furthermore, the complementation of the WT arcA gene from pBAD33-arcA+ was constructed and all of the above features of the pBAD33-arcA+ complemented strain were restored to the WT level. Conclusions We showed the effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides, and demonstrated that ArcA functions as a positive regulator controls the motility of P. shigelloides by directly regulating the expression of flaK, rpoN and cheV genes.

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Plesiomonas shigelloides sipD mutant, generated by an efficient gene transfer system, is less invasive

Cited by 8 publications

References 22 publications

Effects of dietary Bacillus amyloliquefaciens on the growth, immune responses, intestinal microbiota composition and disease resistance of yellow catfish, Pelteobagrus fulvidraco

Effects of dietary Bacillus amyloliquefaciens on the growth, immune responses, intestinal microbiota composition and disease resistance of yellow catfish, Pelteobagrus fulvidraco

RpoN is required for the motility and contributes to the killing ability of Plesiomonas shigelloides

The effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides

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