Platinum Nanoflowers for Surface-Assisted Laser Desorption/Ionization Mass Spectrometry of Biomolecules

Kawasaki, Hideya; Yonezawa, Tetsu; Watanabe, Takeshi; Arakawa, Ryuichi

doi:10.1021/jp075159d

Cited by 175 publications

(140 citation statements)

References 27 publications

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“…Various inorganic materials have been applied for MS analysis, including cobalt powder, graphite powder, porous silicon surface, and metal particles [8]. SALDI not only improves the reproducibility of MS analysis but also reduces the background signals of the organic matrix ions in the low-mass range [9,10]. Recently, nano-materials have attracted considerable attention for SALDI-MS analysis due to their high surface areas, simple sample preparation, and flexible sample deposition conditions.…”

mentioning

confidence: 99%

CHCA-modified Au nanoparticles for laser desorption ionization mass spectrometric analysis of peptides

Duan

Linman

Chen

et al. 2009

J. Am. Soc. Mass Spectrom.

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Gold nanoparticles (AuNPs) have been studied as a potential solid-state matrix for laser desorption/ionization mass spectrometry (LDI-MS) but the efficiency in ionization remains low. In this report, AuNPs are capped by a self-assembled monolayer of cysteamine and modified with ␣-cyano-4-hydroxycinnanic acid (CHCA) for effective MALDI measurements. CHCA-terminated AuNPs offer marked improvement on peptide ionization compared with citrate-capped or cysteamine-capped AuNPs. The coating also effectively suppresses formation of Au cluster ions and analyte fragment ions, leading to cleaner mass spectra. Addition of glycerol and citric acid to the peptide/AuNPs sample further improves the performance of these AuNPs for LDI-MS analysis. Glycerol appears to enhance the dispersion of AuNPs in sample spots, increasing the sample ionization and shot-to-shot reproducibility, while citric acid serves as an external proton donor, providing high production of protonated analyte ions and reducing fragmentation of peptides on the nanoparticle-based surface. Optimal ratios of citric acid, glycerol, and AuNPs in sample solution have been systematically studied. A more than 10-fold increase for desorption ionization of peptides can be achieved by combining 5% glycerol and 20 mM citric acid with the CHCA-terminated AuNPs. The applicability of the CHCA-AuNPs for LDI-MS analysis of protein digests has also been demonstrated. This work shows the potential of AuNPs for SALDI-MS analysis, and the improvement with chemical functionalization, controlled dispersion, and use of an effective proton donor. (J Am Soc Mass

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mentioning

confidence: 99%

CHCA-modified Au nanoparticles for laser desorption ionization mass spectrometric analysis of peptides

Duan

Linman

Chen

et al. 2009

J. Am. Soc. Mass Spectrom.

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“…Unlike the situation for the Au nanomaterials, which produced many Au clusters, we did not detect any Pt clusters when using the Pt NSPs as the nanomaterials, primarily because of the relatively low thermal conductivity of Pt (71.6 W m Ϫ1 K Ϫ1 at 300 K; cf. 318 m Ϫ1 K Ϫ1 for Au) [17]. For small analytes (e.g., glutathione), the Au NPs provided much better sensitivity than did the Pt NSPs, mainly due to more-efficient desorption and ionization.…”

Section: Methodsmentioning

confidence: 99%

“…CdTe QDs provided good sensitivity for analytes in the mass range from 330.07 Da to 12 kDa. Among the six nanomaterials, the Fe 3 O 4 NPs and Pt NSPs provided the greatest sensitivity for larger proteins (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25) [9,10]. When using the Pt NSPs for SALDI-MS analyses, the batch-to-batch variations for the smaller (Ͻ12 kDa) and larger (12-25 kDa) proteins were 16% and 35%, respectively.…”

Section: Methodsmentioning

confidence: 99%

Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and proteins

Chiang

Lin

et al. 2010

J. Am. Soc. Mass Spectrom.

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We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomaterials for small analytes (e.g., glutathione); Pt nanosponges and Fe 3 O 4 NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomaterials have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spots"), when used in laser desorption/ionization mass spectrometry. (J Am Soc Mass Spectrom 2010, 21, 1204 -1207) © 2010 American Society for Mass Spectrometry S urface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) was developed recently using nanomaterials rather than organic compounds as matrices, for the determination of analytes of interest. For example, laser desorption/ionization (LDI) of intact proteins and protein aggregates in the presence of glycerol has been demonstrated using cobalt particles (ca. 30 nm) [1]. Similar to the role played by organic matrices, the particles absorb energy from the laser irradiation and transfer it efficiently to the analytes, thereby inducing desorption and ionization. Mixtures of graphite particles (2-150 m) and glycerol have been employed in the analysis of proteins and peptides [2,3]. Several other nanomaterials, including carbon nanotubes, nanodiamonds, and various nanoparticles (NPs, namely SiO 2 , ZnS, TiO 2 , Fe 3 O 4 , Fe 3 O 4 /TiO 2 , and Au) are also useful-without the addition of glycerol-for SALDI-MS [4 -12]. Because of their unique chemical and physical properties, NPs can also act as selective probes and/or efficient ionization nanomaterials. For example, Au and TiO 2 NPs are suitable for the concentration and ionization of aminothiols and catechins, respectively, in SALDI-MS [8,11]. One other advantage of using NPs is that fewer "sweet spots" are formed, thereby maximizing reproducibility. Although NPs have been used successfully for the determination of a range of analytes (from small analytes to proteins), a review of the literature reveals that the various NPs provide quite different results in terms of sensitivity, reproducibility, and mass range. Thus, our aim in this study was to evaluate the performance of several types of NPs for the analysis of peptides and proteins. ExperimentalSix nanomaterials-Au NPs, TiO 2 NPs, Se NPs, CdTe quantum dots (QDs), Fe 3 O 4 NPs, and Pt nanosponges (NSPs)-were tested for the SALDI-MS-based analyses of peptides and proteins; they were prepared in aqueous solutions and characterized according to procedures described in the literature [8,11,[13][14][15][16]. A twolayer preparation method was applied to deposit the nanomaterials and samples onto the metal plates used in SALDI-MS. First, one of the nanomaterial solutions (1 L) was deposited into one of the wells of the MS plate and dried under ambient cond...

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“…Kawasaki et al applied SALDI MS using Pt nanoflowers for the analyses of various biomolecules, mainly proteins. 64 Further, sulfonate group-modified PtFeCu NPs were employed as selective capture probes for positively charged proteins as well as efficient laser desorption/ionization (LDI) agents. 65 Chiang et al employed HgTe nanostructures as a new matrix for SALDI MS-based analyses of proteins and their complexes with small analytes.…”

Section: Peptides Proteins and Amino Acidsmentioning

confidence: 99%

Analysis of Biomolecules through Surface‐Assisted Laser, Desorption/Ionization Mass Spectrometry Employing Nanomaterials

Chen

Tomalová

Preisler

2011

J Chinese Chemical Soc

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Nanomaterials, primarily nanoparticles (NPs), can serve as an alternative matrix for the analysis of various biomolecules through surface-assisted laser desorption/ionization mass spectrometry (SALDI MS). SALDI MS has been developed to overcome poor reproducibility and high background in the lowmass region commonly occurring in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Various nanomaterials, unmodified or functionalized with recognition ligands, can have a strong affinity toward certain analytes and thus are applicable for their concentration and enrichment from complex biological matrices. In mass spectrometry imaging (MSI), the use of NPs instead of the conventional matrices can improve the spatial resolution up to the cellular level. In this review, the nature of NPs, the methods of sample preparation and approaches for quantitation of biomolecules through SALDI MS are discussed. Practical applications and limitations of SALDI MS employing NPs for separate samples and MSI are mentioned. With regard to the nature of MSI analysis, the use of nanostructured surfaces for MSI is also reflected in this review.

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Platinum Nanoflowers for Surface-Assisted Laser Desorption/Ionization Mass Spectrometry of Biomolecules

Cited by 175 publications

References 27 publications

CHCA-modified Au nanoparticles for laser desorption ionization mass spectrometric analysis of peptides

CHCA-modified Au nanoparticles for laser desorption ionization mass spectrometric analysis of peptides

Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and proteins

Analysis of Biomolecules through Surface‐Assisted Laser, Desorption/Ionization Mass Spectrometry Employing Nanomaterials

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