Platinum black electrodeposited thread based electrodes for dielectrophoretic assembly of microparticles

Abstract

We report dielectrophoretic (DEP) assembly of biological cells and microparticles using platinum-black electrodeposited conductive textile fiber. The threedimensional conductive structures with high aspect ratios were found to facilitate high electric field regions, as revealed by scanning electron microscope characterization. The effective conducting area (A eff ) and its stability of thread electrodes were estimated using electrochemical methods. Potential of platinum black electrodeposited thread as 3-D electrodes for creating high gradient electrical field for dielectrophoretic assembly of microspheres and Saccharomyces cerevisiae (yeast cells) into 1D and two-dimensional structures over long ranges under the application of low voltages (4-10 V pp ) has been demonstrated. The formation of highly ordered pearl chains of microparticles using thread electrodes when subjected to dielectrophoresis (DEP) has been discussed in detail. Published by AIP Publishing.

Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy

“…In order to overcome this problem, additional cell capture mechanisms are required. A large number of methods have been developed to capture cells more effectively including magnetic , optical , hydrodynamic , acoustic , and electrical forces . Using electrical forces to capture cells has been utilized frequently due to its simplicity, high selectivity, contact‐free approach, and its potential to be integrated into microfluidic systems.…”

“…Combination of microwell arrays with dielectrophoretic force, which is the motion of polarizable particles in a nonuniform electric field, enabled researchers to capture biological cells in confined well geometries more effectively in shorter periods of time. Electro‐activated microwells have been used for several applications such as cell sorting and cell manipulation . Kim et al.…”

“…Combination of microwell arrays with dielectrophoretic force, which is the motion of polarizable particles in a nonuniform electric field, enabled researchers to capture biological cells in confined well geometries more effectively in shorter periods of time. Electro-activated microwells have been used for several applications such as cell sorting and cell manipulation [40][41][42][43][44]. Kim et al proposed a microfluidic device made up of electro-active microwells to capture cells using positive dielectrophoresis (pDEP) and then lyse them using electroporation [42].…”

Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis

“…Hence, of particular interest here is the use of 3D electrodes, spanning the height of the microfluidic channel, to improve throughput when compared to more traditional planar devices. 3D electrodes have been implemented using different fabrication techniques 29 such as electroplating, 30,31 metallization of 3D structures, [32][33][34] and casting of conductive resins. 35 Here, we use 3D carbon electrodes to implement dielectrophoresis (carbonDEP) and enrich a yeast population originally diluted in a large sample volume.…”

Enhancement of dielectrophoresis using fractal gold nanostructured electrodes

“…Decreased EP in fractal electrodes is considered as a reason for enhanced dielectrophoretic response. Dielectrophoresis (DEP) is an alternating current (AC) electrokinetic phenomenon used for separation, manipulation, concentration, and characterization of virus, biomolecules, bacteria, circulating tumor cells, and colloidal particles [1][2][3][4][5][6][7][8][9][10]. DEP is the motion of polarizable particles that are suspended in a medium and subjected to a spatially nonuniform electric field.…”