Platelets from platelet-rich-plasma versus buffy-coat-derived platelets: what is the difference?

Gulliksson, H.

doi:10.5581/1516-8484.20120024

Cited by 27 publications

(19 citation statements)

References 6 publications

(6 reference statements)

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“…33 The key strategies for platelet preparation include buffy coat and apheresis methods. [34][35][36][37] The buffy coat method, which was used in this manuscript, is based on centrifugation of the whole blood to separate platelets, leukocytes, erythrocytes and plasma. The excess erythrocytes and leukocytes are removed via specific filtration.…”

mentioning

confidence: 99%

Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Rampichová

Buzgo

Míčková

et al. 2017

IJN

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mentioning

confidence: 99%

Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Rampichová

Buzgo

Míčková

et al. 2017

IJN

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“…In contrast BC method gives the luxury of cushion to platelets among RBC during the hard spin. Therefore platelets are less activated than PRP method [6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Random Donor Platelets Produced from Buffy Coat Stored for 24 h at Ambient Temperature: Should This be Implemented in India?

Philip

Samantha

Chatterjee

et al. 2014

Indian J Hematol Blood Transfus

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Whole blood derived platelets are made from platelet rich plasma (PRP) method or buffy coat (BC) method. In India majority of random donor platelets (RDPs) are prepared by PRP method. However, BC method offers the advantage of less platelet activation and fewer WBC contamination. Presently in India RDPs are prepared within 8 h of whole blood collection, whereas, in Europe this time limit is up to 24 h. Our aim was to evaluate the platelet count, WBC contamination, platelet CD62P expression, and biochemical parameters of RDPs prepared from BC within 8 h and within 24 h of collection. We prepared 40 units of RDP by the BC method from whole blood stored at room temperature within 8 h of collection (fresh BC), and another 40 units from BC stored at 22 °C for <24 h (stored BC). We analyzed the platelet counts, CD62P expression, WBC counts, glucose levels, pH, PO2, PCO2 in both the groups of RDPs, 24 h after respective preparation. The platelet counts from stored BC was higher in fresh BC. CD62P expression was low in stored BC compared to fresh BC. There were no differences of pH, pO2, pCO2 and glucose levels in fresh BC and stored BC. WBC contamination was more in fresh BC. Our study stored BC contained higher platelet counts, less WBC contamination and less platelet activation. We conclude that RDP prepared from stored BC is the better method for RDP production.

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“…The growth factors are contained in the alpha granules of the platelets. They can be released by addition of calcium chloride, allogeneic thrombin or by autologous fibrin depending on the preparation technique . Other than individual recombinant growth factors, PCs convey a number of signals that may vary in their relative strength according to the dose levels present in the individual preparations.…”

Section: Introductionmentioning

confidence: 99%

Clinical Efficacy of Growth Factors to Enhance Tissue Repair in Oral and Maxillofacial Reconstruction: A Systematic Review

Schliephake

2013

Clinical Implant Dentistry and Related Research

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Platelets from platelet-rich-plasma versus buffy-coat-derived platelets: what is the difference?

Cited by 27 publications

References 6 publications

Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Evaluation of Random Donor Platelets Produced from Buffy Coat Stored for 24 h at Ambient Temperature: Should This be Implemented in India?

Clinical Efficacy of Growth Factors to Enhance Tissue Repair in Oral and Maxillofacial Reconstruction: A Systematic Review

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Platelets from platelet-rich-plasma versus buffy-coat-derived platelets: what is the difference?

Cited by 27 publications

References 6 publications

Platelet-functionalized three-dimensional poly-&epsilon;-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Platelet-functionalized three-dimensional poly-&epsilon;-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Evaluation of Random Donor Platelets Produced from Buffy Coat Stored for 24 h at Ambient Temperature: Should This be Implemented in India?

Clinical Efficacy of Growth Factors to Enhance Tissue Repair in Oral and Maxillofacial Reconstruction: A Systematic Review

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Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors