Platelet viability following storage for 5 days

Abstract

Regional blood centers frequently need to hold units of whole blood at 20 to 24 degrees C for several hours after phlebotomy so that sufficient platelet concentrates can be prepared to meet the increasing need. We have evaluated the in vivo viability and in vitro properties of platelets that were prepared from whole blood drawn into citrate-phosphate-dextrose-adenine (CPDA-1) either immediately after phlebotomy or after an 8-hour hold at 20 to 24 degrees C. Platelet concentrates were stored for 5 days at 20 to 24 degrees C in polyolefin containers (PL 732, Fenwal) with end-over-end tumbler agitation. The autologous in vivo recovery (mean +/- SD) and one-half disappearance of 51Cr-labeled platelets prepared immediately after phlebotomy were 44.4 +/- 9.4 percent and 4.0 +/- 0.5 days, respectively. Platelets prepared after the delay of 8 hours showed a recovery of 44.5 +/- 8.4 percent and a one-half disappearance of 4.1 +/- 0.4 days. After 5 days of storage, platelet concentrates showed a mean pH of 7.21 +/- 0.20 when prepared immediately after phlebotomy, and of 7.22 +/- 0.15 when prepared after an 8-hour delay. Mean morphology scores were 280 +/- 33 and 302 +/- 27 for platelets from units prepared immediately after phlebotomy or after a holding period of 8 hours, respectively. Platelets underwent synergistic aggregation after 5 days of storage, independent of the length of time that the units of whole blood were held prior to centrifugation. These studies indicate that platelet concentrates prepared from units of whole blood held initially for 8 hours can be stored for 5 days at 20 to 24 degrees C and survive satisfactorily in vivo and retain in vitro characteristics.

Studies on the Procurement of Blood Coagulation Factor VIII in vitro Studies on Blood Components Prepared in Half-Strength Citrate Anticoagulant

“…These recovery and survival studies have been initiated. It has also been shown that red cell and platelet preparations from standard CPD.Al dona tions may be prepared 8-15 h after donation [43][44][45]. It will be of interest to determine if this is also possible for half-strength citrate donations and if so whether the improved stability of Vili : C already demonstrated for blood (table II) and plasma ( fig.…”

Studies on the Procurement of Blood Coagulation Factor VIII

“…Other workers have already demonstrated that red cell and platelet preparations may be prepared up to 8 [6,[9][10][11][12][13][14][15] or 15 h [ 16] after collection of blood in CPD anti coagulants. The in vitro data presented here indicate that red cell and platelet preparations of good quality may be prepared 18 h after collection in 0.5 CPD.A2.…”

Aggregability and Post-Transfusion Survival of Canine Platelets in Stored Whole Blood

“…In human medicine, PCs are usually used for platelet administration and PCs are transfused within 72 hr after blood drawing [6,15,20]. Even the lyophilized dry technique of platelets was investigated to extend storage time [19].…”

“…Changes of platelet aggregability were assessed in this study also. Because platelet aggregation in CPDA-1 treated PRP is much weaker than that in 0.38% sodium citrate treated PRP [15], CaCl 2 was added to counteract the excess citrate and therefore enhance aggregation. Groh et al [10] also performed the re-calcification technique for platelet aggregometry using PRP treated with acid citrate dextrose, also a blood cell preservative but not an ideal anticoagulant for platelet aggregometry.…”

“…Collagen (Chrono-collagen, Chrono-log) and adenosine diphosphate (ADP; Chrono-ADP, Chrono-log) were used as the agonists. Because CPDA-1 is not an ideal anticoagulant for aggregometry [15], the stimulating conditions were modified as follows to evaluate the platelet responses against a single agonist: Final concentrations of collagen and ADP were 50 µg/ml and 50 µM respectibly. In addition, 4 mM of CaCl 2 (using 100 mM solution) was added prior to ADP stimulation.…”