Platelet sequestration with a new‐generation autotransfusion device

Seyfried, Timo; Streithoff, Fabian; Gruber, Michael; Unterbuchner, Christoph; Zech, Nina; Kieninger, Martin; Hansen, Ernil

doi:10.1111/trf.14491

Cited by 8 publications

(5 citation statements)

References 36 publications

(26 reference statements)

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“…In clinical practice, IOCS has the following advantages[ 19 - 21 ]: (1) It can relieve the increasingly tight blood supply and does not require blood type identification and cross-matching, which is convenient and safe; and (2) It can prevent the spread of infectious diseases and adverse reactions caused by allogeneic blood transfusion. If IOCS has high operational requirements, the collection and transfusion of blood should follow the principles of aseptic operation to reduce the risk of cross-infection.…”

Section: Discussionmentioning

confidence: 99%

Effect of intraoperative cell rescue on bleeding related indexes after cesarean section

Yu¹,

Cao²

2022

WJCC

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BACKGROUND Obstetric hemorrhage is the leading cause of maternal mortality globally, especially in China. The key to a successful rescue is immediate and rapid blood transfusion. Autotransfusion has become an integral part of clinical blood transfusion, with intraoperative cell salvage (IOCS) being the most widely used. AIM To investigate the application of IOCS in cesarean section. METHODS A total of 87 patients who underwent cesarean section and blood transfusion in our hospital from March 2015 to June 2020 were included in this prospective controlled study. They were divided into the observation (43 cases) and control (44 cases) groups using the random number table method. The patients in both groups underwent lower-segment cesarean section. The patients in the control group were treated with traditional allogeneic blood transfusion, whereas those in the observation group were treated with IOCS. Hemorheology [Red blood cell count, platelet volume, and fibrinogen (FIB)] and coagulation function (partial prothrombin time, prothrombin time (PT), platelet count, and activated coagula-tion time) were measured before and 24 h after transfusion. In the two groups, adverse reactions, such as choking and dyspnea, within 2 h after cesarean section were observed. RESULTS Before and after transfusion, no significant differences in hemorheology and coagulation function indices between the two groups were observed ( P > 0.05). About 24 h after transfusion, the erythrocyte count, platelet ratio, and FIB value significantly decreased in the two groups ( P < 0.05); the PLT value significantly decreased in the two groups; the activated partial thromboplastin time, PT, and activated clotting time significantly increased in the two groups ( P < 0.05); and no statistical differences were observed in hemorheology and coagulation function indices between the two groups ( P > 0.05). Furthermore, there was no significant difference in the incidence of adverse reactions between the two groups ( P > 0.05). CONCLUSION In patients undergoing cesarean section, intraoperative cell salvage has a minimum effect on hemorheology and coagulation function and does not increase the risk of amniotic fluid embolism.

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Section: Discussionmentioning

confidence: 99%

Effect of intraoperative cell rescue on bleeding related indexes after cesarean section

Yu¹,

Cao²

2022

WJCC

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“…This subject established scientific and rigorous in vitro and in vivo models and adopted strict variable control methods to study the relationship between autologous reinfusion of red blood cells and CD8+ T cell proliferation [ 11 ]. Through basic and effective cell experiments and biochemical characterization methods, it has been proved that the membrane proteins CD28 and MHC on the surface of autologous reinfused red blood cells can bind to CD8+T cells, thereby promoting the proliferation of CD8+T cells and inhibiting the malignant transformation of liver cancer.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanism Exploration of Autologous Blood Transfusion with RBC Surface Membrane Protein pMHC/aCD28 Combined with CD8+T Cells to Promote the Proliferation of CD8+T Cells to Inhibit the Malignant Transformation of Liver Cancer

Tao

Liu

Yao

et al. 2022

Journal of Oncology

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Autologous blood transfusion is an important blood protection measure. Red blood cells have a certain degree of immunogenicity and their surface membrane proteins CD28 and MHC can participate in the immune response and interact with CD8+ T cells. We build a cell model with a transwell system. The binding characteristics of RBCs and CD8+ T cells were observed with a fluorescent confocal microscope. The content of the inflammatory factor TNF-α and IFN-γ produced was analyzed by ELISA. The proliferation characteristics of CD8+ T cells were analyzed by CFSE staining, and the content of CD3+CD8+ T cells was analyzed by flow cytometry. Cell migration and invasion experiments were used to analyze the malignant metastasis ability of liver cancer cells. The expression of vimentin, E-cadherin, and β-catenin was analyzed by Western blot. We establish a liver cancer model in rats and group them for autologous blood transfusion. The content of CD3+CD8+T cells in the blood of each group of rats was analyzed by flow cytometry. Western blot was used to analyze the expression of vimentin, E-cadherin, and β-catenin in the liver tissues of rats in each group. The red blood cells in the autologous reinfusion blood and CD8+ T cells have an obvious combination. The degree of combination of the two is related to the expression of CD28 and MHC. If CD28 and MHC are expressed at the same time, the combination of the two cells will be high, the proliferation of CD8+ T cells will increase, and the expression of inflammatory factors will also increase, while the expression of the three proteins that are positively correlated with the activity of cancer cells will decrease. If only one of CD28 and MHC is normally expressed, the result is contrary to the situation where both membrane proteins are normally expressed. Our project has proved that autologous infusion of red blood cell surface membrane proteins CD28 and MHC combined with CD8+ T cells can promote the proliferation of CD8+ T cells to inhibit the malignant transformation of liver cancer.

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“…Finally, autotransfusion devices have also been employed to separate platelets from platelet-rich plasma. Three types of autotransfusion devices utilizing either a Latham bowl, Baylor bowl, or closed-spiral chamber, showed only 50%–60% platelet recovery,51 which was consistently lower than that for conventional centrifugation 52. The platelet recovery performance of autotransfusion devices will have to be significantly improved before they could provide a practical alternative to specialized cell processors or manual centrifugation.…”

Section: Centrifugation-based Technologiesmentioning

confidence: 97%

Traditional and emerging technologies for washing and volume reducing blood products

Lu¹,

Lezzar²,

Vörös³

et al. 2019

JBM

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Millions of blood components including red blood cells, platelets, and granulocytes are transfused each year in the United States. The transfusion of these blood products may be associated with adverse clinical outcomes in some patients due to residual proteins and other contaminants that accumulate in blood units during processing and storage. Blood products are, therefore, often washed in normal saline or other media to remove the contaminants and improve the quality of blood cells before transfusion. While there are numerous methods for washing and volume reducing blood components, a vast majority utilize centrifugation-based processing, such as manual centrifugation, open and closed cell processing systems, and cell salvage/autotransfusion devices. Although these technologies are widely employed with a relatively low risk to the average patient, there is evidence that centrifugation-based processing may be inadequate when transfusing to immunocompromised patients, neonatal and infant patients, or patients susceptible to transfusion-related allergic reactions. Cell separation and volume reduction techniques that employ centrifugation have been shown to damage blood cells, contributing to these adverse outcomes. The limitations and disadvantages of centrifugation-based processing have spurred the development of novel centrifugation-free methods for washing and volume reducing blood components, thereby causing significantly less damage to the cells. Some of these emerging technologies are already transforming niche applications, poised to enter mainstream blood cell processing in the not too distant future.

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Platelet sequestration with a new‐generation autotransfusion device

Abstract: The autotransfusion device XTRA was capable of high-quality perioperative PRP preparation, and the bowl size was found to have an influence on the efficiency of the preparation.

Cited by 8 publications

References 36 publications

Effect of intraoperative cell rescue on bleeding related indexes after cesarean section

Effect of intraoperative cell rescue on bleeding related indexes after cesarean section

Molecular Mechanism Exploration of Autologous Blood Transfusion with RBC Surface Membrane Protein pMHC/aCD28 Combined with CD8+T Cells to Promote the Proliferation of CD8+T Cells to Inhibit the Malignant Transformation of Liver Cancer

Traditional and emerging technologies for washing and volume reducing blood products

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