Platelet-Rich Plasma Provides Nucleus for Mineralization in Cultures of Partially Differentiated Periodontal Ligament Cells

Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

“…A previous study by Kawase et al 36) showed that plateletrich plasma (PRP) increased the ALP activity of hPDL cells and the expression of osteogenic markers. However, PRP did not induce full differentiation into bone cells, and thus a clear indication of incomplete mineralization.…”

Periodontal ligament cell sheet promotes periodontal regeneration in athymic rats

“…Other attempts for regeneration were based on the use of fibroblast growth factor in combination with BMP‐2, and periosteum cells enhanced the proliferation rate and subsequent bone formation (Agata et al 2007). Combination of growth factors such as platelet‐rich‐plasma preparations were also shown to be capable of promoting regeneration of connective tissues (Kawase et al 2005, Yamada et al 2006). However, these procedures were mainly focused on bone formation and the clinical outcomes were still insufficient.…”

Platelet Gel Supplementation in Long Bone Nonunions Treated by External Fixation

“…Nevertheless, evidence was provided that high levels of growth factors can be detected also in PRP produced by different methods including 1 or more cycles of freezing, 22 and frozen PRP was proved to be as effective as freshly prepared PRP in providing platelet aggregates as nuclei to initiate mineralization while stimulating periodontal ligament cells in vitro. 23 Moreover, freeze-dried platelets and PRP have been shown to improve wound healing in mice. 24,25 Despite these positive results, the effect of freezing on the biologic activity of platelet growth factors could be hypothesized as a possible factor affecting the efficacy of PG in the present study because of the peculiar biology of long bone nonunions.…”