Platelet‐rich plasma on calcium phosphate granules promotes metaphyseal bone healing in mini‐pigs

Abstract

ABSTRACT:The role of platelet-rich plasma (PRP) as a promoter of bone healing remains controversial. The aim of this study was to investigate the effect of PRP in combination with calcium phosphate granules (CPG) on bone defect healing in a metaphyseal long bone defect. A metaphyseal bone defect at the proximal tibia of 16 mini-pigs was filled with CPG combined with autologous PRP or CPG solely (control group). The PRP showed 4.4-fold more platelets compared to peripheral blood. Six weeks after surgery the radiological and histomorphometrical evaluations showed significantly more bone formation in the PRP group in the central area of the defect zone (p < 0.01) as well as the cortical defect zone (p < 0.04). Furthermore, the resorption rate of CPG was increased in animals who received PRP. Nevertheless there were only isolated instances of complete osseous bridging of the bone defects even in the PRP group. This study demonstrates that a PRP-CPG composit promotes bone regeneration but does not lead to a solid fusion of a tibial defect in mini-pigs.

BMP4 localization and PCNA expression during distraction osteogenesis of the porcine mandible

“…[26][27][28][29][30] A large animal model also allows the study of specific areas of the DO wound. Minipigs are cooperative and relatively easy to work with during the experimental manipulations.…”

Comparison of Platelet-Rich Plasma and VEGF-Transfected Mesenchymal Stem Cells on Vascularization and Bone Formation in a Critical-Size Bone Defect

“…Recently there have been conflicting reports about the effect of PRP plasma, and there is no consensus regarding the role of PRP in bone regeneration: there are multiple favorable results of PRP regarding the improvement of proliferation of MSC in vitro and in vivo after ectopic implantation, but also in animal models with critical-size bone defects [Kasten et al, 2008b;Bi et al, 2010;Hakimi et al, 2010;Hartmann et al, 2010;Jungbluth et al, 2010;Niemeyer et al, 2010;Bae et al, 2011;Liu et al, 2011]. However, there is also a relevant number of studies reporting no or minimal effects of PRP on bone formation [Li et al, 2004;Roldan et al, 2004;Plachokova et al, 2007;Thor et al, 2007;Vasconcelos Gurgel et al, 2007;You et al, 2007;Miloro et al, 2010;Kazakos et al, 2011].…”

Healing of massive segmental femoral bone defects in minipigs by allogenic ASCs engineered with FLPo/Frt-based baculovirus vectors

“…Besides the aforementioned animals, minipig is a recommended large animal model for pre-clinical evaluation of human orthopedic implants [35], and has been employed to assess bone repair at different sites including tibia [36], calvaria [37], mandible [38] and cartilage [39,40]. Yet these studies focused on the evaluation of cells, scaffold materials and growth factors, alone or in combination.…”